scholarly journals Identification of fatty acid synthetase messenger RNA on free polyribosomes isolated from lactating rabbit mammary gland

1981 ◽  
Vol 199 (3) ◽  
pp. 789-793 ◽  
Author(s):  
M Antoniou ◽  
R Craig ◽  
R Dils
Keyword(s):  
Molecular Weight ◽  
Mammary Gland ◽  
Fatty Acid ◽  
Messenger Rna ◽  
Fatty Acid Synthetase ◽  
Lower Molecular Weight ◽  
Molecular Weight Range ◽  
Weight Range ◽  
Run Off ◽  
Immunochemical Identification

The synthesis of fatty acid synthetase on free polyribosomes from lactating rabbit mammary gland was demonstrated by using polyribosomes run-off techniques and immunochemical identification of products with synthetase antiserum. Several reproducible and discrete immunoprecipitable polypeptides were observed which were within the molecular-weight range of the synthetase subunit (235 000--252 000), as well as several of lower molecular weight.

scholarly journals Molecular weight and subunit size of fatty acid synthetase from rabbit mammary gland

1976 ◽  
Vol 159 (1) ◽  
pp. 181-184 ◽  
Author(s):  
N Paskin ◽  
R J Mayer
Keyword(s):  
Molecular Weight ◽  
Mammary Gland ◽  
Fatty Acid ◽  
Sodium Dodecyl Sulphate ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Gel Filtration ◽  
Fatty Acid Synthetase ◽  
Subunit Size

Fatty acid synthetase purified from the mammary gland of the rabbit has a mol. wt. of 968000 as determined by gel filtration. The enzyme gave one band, corresponding to a mol.wt. of approx. 35000, on polyacrylamide-gel electrophoresis in the presence of sodium dodecyl sulphate and phenylmethanesulphonyl fluoride.

scholarly journals The synthesis of medium-chain fatty acids by lactating-rabbit mammary gland studied in vitro (Short Communication)

1973 ◽  
Vol 132 (1) ◽  
pp. 121-123 ◽  
Author(s):  
Christopher R. Strong ◽  
Eric M. Carey ◽  
Raymond Dils
Keyword(s):  
Fatty Acids ◽  
Molecular Weight ◽  
Mammary Gland ◽  
Fatty Acid ◽  
Short Communication ◽  
Acyl Chain ◽  
Fatty Acid Synthetase ◽  
Supernatant Fraction ◽  
Weight Factor

The proportion of C8:0 and C10:0 fatty acids synthesized by the microsomal plus particle-free supernatant fraction from lactating rabbit mammary gland is enhanced at high protein concentrations. This fraction appears to contain a soluble high-molecular-weight factor that modifies the specificity of the fatty acid synthetase complex for termination of the growing acyl chain.

scholarly journals Molecular weight and subunit size of rabbit mammary-gland fatty acid synthetase

1978 ◽  
Vol 173 (3) ◽  
pp. 929-933 ◽  
Author(s):  
I Grunnet ◽  
J Knudsen
Keyword(s):  
Molecular Weight ◽  
Mammary Gland ◽  
Fatty Acid ◽  
Sucrose Gradient ◽  
Gradient Centrifugation ◽  
Fatty Acid Synthetase ◽  
Mammary Glands ◽  
Sucrose Gradient Centrifugation ◽  
Molecular Weights ◽  
Subunit Size

1. The molecular weights of fatty acid synthetases isolated from lactating rabbit, rat, cow and goat mammary glands were estimated by sucrose gradient centrifugation and compared by chromatography on Sepharose 6B. 2. The values obtained for all four enzymes were in the same range (0.40 × 10(6)-0.55 × 10(6)) as that found for other mammalian and avian fatty acid synthetases. The molecular weight found for the rabbit mammary enzyme therefore differs from published values of approx. 0.9 × 10(6). 3. The molecular weights of the subunits of these four synthetases were 225000-242000. Again, the value for the rabbit mammary enzyme differs from published values.

Mammary gland fatty acid synthetase messenger RNA

1985 ◽  
Vol 13 (5) ◽  
pp. 830-831 ◽  
Author(s):  
RAYMOND R. DILS ◽  
IAIN R. CAMERON ◽  
CHRISTOPHER J. SKIDMORE ◽  
ALPHONS F. WEIR
Keyword(s):  
Mammary Gland ◽  
Fatty Acid ◽  
Messenger Rna ◽  
Fatty Acid Synthetase

scholarly journals Nuclear matrix of HeLa S3 cells. Polypeptide composition during adenovirus infection and in phases of the cell cycle.

1977 ◽  
Vol 72 (1) ◽  
pp. 194-208 ◽  
Author(s):  
L D Hodge ◽  
P Mancini ◽  
F M Davis ◽  
P Heywood
Keyword(s):  
Cell Cycle ◽  
Molecular Weight ◽  
Nuclear Matrix ◽  
Apparent Molecular Weight ◽  
Molecular Weight Range ◽  
Weight Range ◽  
Molecular Weights ◽  
Liver Nuclei ◽  
Hela S3 ◽  
Biochemical Analyses

A subnuclear fraction has been isolated from HeLa S3 nuclei after treatment with high salt buffer, deoxyribonuclease, and dithiothreitol. This fraction retains the approximate size and shape of nuclei and resembles the nuclear matrix recently isolated from rat liver nuclei. Ultrastructural and biochemical analyses indicate that this structure consists of nonmembranous elements as well as some membranous elements. Its chemical composition is 87% protein, 12% phospholipid, 1% DNA, and 0.1% RNA by weight. The protein constituents are resolved in SDS-polyacrylamide slab gels into 30-35 distinguishable bands in the apparent molecular weight range of 14,000 - 200,000 with major peptides at 14,000 - 18,000 and 45,000 - 75,000. Analysis of newly synthesized polypeptides by cylindrical gel electrophoresis reveals another cluster in the 90,000-130,000 molecular weight range. Infection with adenovirus results in an altered polypeptide profile. Additional polypeptides with apparent molecular weights of 21,000, 23,000, and 92,000 become major components by 22 h after infection. Concomitantly, some peptides in the 45,000-75,000 mol wt range become less prominent. In synchronized cells the relative staining capacity of the six bands in the 45,000-75,000 mol wt range changes during the cell cycle. Synthesis of at least some matrix polypeptides occures in all phases of the cell cycle, although there is decreased synthesis in late S/G2. In the absence of protein synthesis after cell division, at least some polypeptides in the 45,000-75,000 mol wt range survive nuclear dispersal and subsequent reformation during mitosis. The possible significance of this subnuclear structure with regard to structure-function relationships within the nucleus during virus replication and during the life cycle of the cell is discussed.

Uremic Neurotoxin in the Middle Molecular Weight Range

1980 ◽  
Vol 4 (2) ◽  
pp. 116-120 ◽  
Author(s):  
N.K. Man ◽  
G. Cueille ◽  
J. Zingraff ◽  
J. Boudet ◽  
A. Sausse ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Molecular Weight Range ◽  
Weight Range

Aqueous polypropylene glycol induces swelling and severe plasticization of high Tg amphiphilic copolymers containing hexafluoroisopropanol groups

Soft Matter ◽  
2020 ◽  
Vol 16 (27) ◽  
pp. 6362-6370
Author(s):  
Siyuan Li ◽  
Bryan D. Vogt
Keyword(s):  
Molecular Weight ◽  
Propylene Glycol ◽  
Polypropylene Glycol ◽  
Amphiphilic Copolymers ◽  
Molecular Weight Range ◽  
Weight Range ◽  
Poly Propylene

Not too big, not too small, but a narrow molecular weight range for poly(propylene glycol) where swelling of the copolymer increases tremendously for poly(propylene glycol).

scholarly journals Regulation of enzyme turnover during tissue differention. Studies on the effects of hormones on the turnover of fatty acid synthetase in rabbit mammary gland in organ culture

1975 ◽  
Vol 148 (2) ◽  
pp. 309-320 ◽  
Author(s):  
B K Speake ◽  
R Dils ◽  
R J Mayer
Keyword(s):  
Mammary Gland ◽  
Fatty Acid ◽  
Organ Culture ◽  
Half Life ◽  
Culture Medium ◽  
Fatty Acid Synthetase ◽  
Enzyme Complex ◽  
Rapid Fall ◽  
Enzyme Turnover ◽  
Rabbit Milk

1. Explants of mammary gland from mid-pregnant rabbits were cultured with insulin, prolactin and cortisol. 2. Antibodies raised to fatty acid synthetase were used to measure the amount as well as the rate of synthesis and the rate of degradation of the enzyme in the explants over defined periods in organ culture. These measurements were also made after the hormones had been removed from the culture medium. The changes which occur in the activity of fatty acid synthetase are due to changes in the amount of the enzyme present. They are not due to activation or inactivation of the enzyme. 3. The rate of lipogenesis (measured from [1-14C]acetate) in the explants during culture varies independently of the amount of fatty acid synthetase both in the presence and after removal of the hormones. Hence the amount of fatty acid synthetase does not limit lipogenesis. The proportion of medium-chain fatty acids C8:0 and C10:0 (which are characteristic of rabbit milk) synthesized by the explants in the presence of hormones increases at about the same rate as the amount of fatty acid synthetase present. However, when hormones are removed from the medium the proportion of these acids synthesized declines as rapidly as the rate of lipogenesis and not as the amount of fatty acid synthetase presen. 4. The rates of synthesis of fatty acid synthetase and of the total particulate-free supernatant protein in the explants were compared by measuring the incorporation of L-[U-14C]leucine into the protein of the explants. These rates increase by 5-fold and 3.6-fold respectively when explants are cultured with hormones, and they then reach approximately constant rates. When the hormones are removed there is a rapid fall in the rate of synthesis of fatty acid synthetase and of the total particulate-free supernatant protein to values which are similar to those obtained with freshly prepared explanted tissue. 5. In unstimulated explants fatty acid synthetase appears to be degraded with a half-life of 15-21h. During the hormonally stimulated differentiation of the tissue the rate of degradation of the enzyme is considerably decreased or is switched off completely. After the amount of fatty acid synthetase has increased to a maximum the enzyme complex is again degraded with a half-life of 23-29h. The removal of hormones after the explants have been hormonally stimulated for different times results in an increase in the rate of degradation of fatty acid synthetase. However, this increase only occurs if degradation was previously proceeding at a considerably decreased rate. The degradation of the total particulate-free supernatant protein continues throughout the period of differentiation of the explant tissue in culture. It appears to be somewhat decreased during the period of rapid maturation of the tissue during culture.

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