scholarly journals A comparison of the relationships between progestin receptors and oestrogen receptors in neural and non-neural target tissues of the rat during the oestrous cycle

1981 ◽  
Vol 196 (3) ◽  
pp. 903-903
Keyword(s):  
Oestrous Cycle ◽  
Oestrogen Receptors ◽  
Progestin Receptors

scholarly journals A comparison of the relationships between progestin receptors and oestrogen receptors in neural and non-neural target tissues of the rat during the oestrous cycle

1980 ◽  
Vol 190 (3) ◽  
pp. 691-695 ◽  
Author(s):  
S Thrower ◽  
L Lim
Keyword(s):  
Cerebral Cortex ◽  
Oestrogen Receptor ◽  
Oestrous Cycle ◽  
Oestrogen Receptors ◽  
Progestin Receptors ◽  
Progestin Receptor ◽  
Cyclic Changes ◽  
Relationship Of ◽  
The Relationship

Similar cyclic changes in the content of nuclear oestrogen receptor occurred in the hypothalamus, cerebral cortex, uterus and pituitary during the oestrous cycle. The relationship of the unoccupied to the total nuclear oestrogen receptor at each phase was similar in all these tissues. However, cyclic changes in the content of the cytosol progestin receptor occurred only in the uterus and pituitary (where they paralleled changes in the nuclear oestrogen receptor), but not in the hypothalamus or cerebral cortex.

Growth of mouse endometrial luminal epithelial cells in vitro: functional integrity of the oestrogen receptor system and failure of oestrogen to induce proliferation

1991 ◽  
Vol 128 (1) ◽  
pp. 115-120 ◽  
Author(s):  
F.-D. A. Uchima ◽  
M. Edery ◽  
T. Iguchi ◽  
H. A. Bern
Keyword(s):  
Epithelial Cells ◽  
Oestrogen Receptor ◽  
Continuous Exposure ◽  
Oestrogen Receptors ◽  
Serum Free Medium ◽  
Free Medium ◽  
Receptor System ◽  
Serum Free ◽  
Progestin Receptors ◽  
Luminal Epithelial Cells

ABSTRACT Normal endometrial luminal epithelial cells isolated from ovariectomized approximately 40-day-old BALB/cCrgl mice were purified by Percoll density gradient centrifugation and grown as primary cultures in collagen gel matrix and serum-free medium. Cells increased threefold in number during the 9-day culture period. Deletion of insulin, epidermal growth factor or bovine serum albumin resulted in decreased growth. Addition of any single factor to the unsupplemented medium had no effect. Relatively high levels of cytosolic oestrogen receptors and progestin receptors were demonstrable in the cultures. Addition of oestrogen did not enhance epithelial cell proliferation. On the contrary, all doses of oestrogen (180 fmol/l to 218 nmol/l) were inhibitory. Continuous exposure to oestradiol-17β (1·8 nmol/l) for 9 days in serum-free medium resulted in a decrease in cytosolic oestrogen receptors with an associated nuclear accumulation of oestrogen receptors. A corresponding increase in cytosolic progestin receptors was also observed, indicating that no qualitative modification of the oestrogen receptor system had occurred. Thus, as previously reported for vaginal epithelial cells, oestrogen, despite its stimulation of specific product synthesis (progestin receptors), did not increase proliferation of endometrial luminal epithelial cells in this culture system. Journal of Endocrinology (1991) 128, 115–120

Localization of Oestrogen Receptors Within Various Bovine Ovarian Cell Types at Different Stages of the Oestrous Cycle

2006 ◽  
Vol 35 (5) ◽  
pp. 334-342 ◽  
Author(s):  
M. D'Haeseleer ◽  
P. Cornillie ◽  
P. Simoens ◽  
W. Van Den Broeck
Keyword(s):  
Cell Types ◽  
Oestrous Cycle ◽  
Oestrogen Receptors ◽  
Ovarian Cell

Are oestrogen receptors and protein tyrosine kinases involved in phytoestrogen-modulated steroid secretion by porcine adrenocortical cells?

2012 ◽  
Vol 60 (2) ◽  
pp. 285-295
Author(s):  
Barbara Kaminska ◽  
Marek Opalka ◽  
Renata Ciereszko ◽  
Luiza Dusza
Keyword(s):  
Tyrosine Kinases ◽  
Protein Tyrosine Kinase ◽  
Oestrous Cycle ◽  
Protein Tyrosine Kinases ◽  
Cortisol Secretion ◽  
Oestrogen Receptors ◽  
Observable Effect ◽  
Adrenocortical Cells ◽  
Protein Tyrosine ◽  
Induced Changes

The phytoestrogens genistein and daidzein had been found to affect the function of some tissues via oestrogen receptors (ER). In addition, genistein, but not daidzein, is considered to be a protein tyrosine kinase (PTK) inhibitor. Thus, the involvement of oestrogen receptors and PTK in phytoestrogen action on adrenocortical porcine steroidogenesis was examined in this study. The aims of the experiment were to test the effects of (i) ICI 182, 780 (ICI), an ER antagonist, on genistein- and daidzein-modulated cortisol and androstenedione (A4) secretion by adrenocortical cells isolated during the luteal and follicular phases of the porcine oestrous cycle; (ii) tyrphostin AG 957 (TAG), a nonsteroidal PTK inhibitor, on cortisol and A4 secretion by the cells and (iii) the phase of the porcine oestrous cycle on the mechanism of phytoestrogen action. Adrenals were harvested during the luteal (n = 5 animals) and follicular (n = 5 animals) phases of the oestrous cycle from locally slaughtered crossbred gilts. The isolated adrenocortical cells were incubated for 8 h (37 °C, 95% air, 5% CO2) with genistein (5 or 10 μM) or daidzein (5 or 10 μM) in the presence or absence of ICI (0.5 μM) or TAG (5 or 10 μM). Genistein and daidzein inhibited cortisol secretion and stimulated A4 secretion by porcine adrenocortical cells harvested during both the luteal and follicular phases of the oestrous cycle. The ER antagonist ICI did not eliminate phytoestrogen-induced changes in steroidogenesis. In contrast to genistein, TAG reduced the secretion of A4 and did not affect cortisol secretion. There was no observable effect due to the phase of the cycle. It is suggested that the mechanism of genistein and daidzein action in the adrenocortical cells of pigs is independent of ER and PTK. It is possible that PTK are involved in A4 secretion by porcine adrenocortical cells.

Circulating reproductive hormones and hypothalamic oestradiol and progestin receptors in infertile Zucker rats

1989 ◽  
Vol 120 (2) ◽  
pp. 331-336 ◽  
Author(s):  
E. M. Whitaker ◽  
A. C. Robinson
Keyword(s):  
Preoptic Area ◽  
Plasma Concentrations ◽  
Underlying Mechanism ◽  
Reproductive Hormones ◽  
Oestrous Cycle ◽  
Zucker Rats ◽  
Medial Basal Hypothalamus ◽  
Progestin Receptors ◽  
Obese Rats ◽  
The Difference

ABSTRACT Plasma concentrations of LH, FSH, prolactin and progesterone were measured during the oestrous cycle in obese (fa/fa) and non obese (Fa/?) Zucker rats. In obese rats the mid-afternoon surge of LH during prooestrus was reduced compared with that in non-obese rats (P<0.05), and the maximum concentrations of FSH and prolactin declined more slowly during oestrus. Progesterone concentrations were higher during most of the oestrous cycle in obese rats. Oestradiol and progestin receptors were measured in the hypothalamus of female Zucker rats. Lower concentrations of oestradiol receptors were found in the preoptic area of obese rats (P<0.05). Concentrations of oestradiol receptors in the medial basal hypothalamus were also lower in obese rats, though the difference was not statistically significant. Concentrations of progestin receptors were similar in both phenotypes in the preoptic area and media basal hypothalamus. It seems likely that the abnormalities in reproductive hormones and oestradiol receptors contribute to the infertility of obese female Zucker rats. The underlying mechanism has still to be determined. Journal of Endocrinology (1989) 120, 331–336

scholarly journals The unoccupied nuclear oestradiol receptor in the rat uterus and hypothalamus during the oestrous cycle

1981 ◽  
Vol 194 (3) ◽  
pp. 667-671 ◽  
Author(s):  
S Thrower ◽  
C Neethling ◽  
J O White ◽  
L Lim
Keyword(s):  
Nuclear Receptor ◽  
Oestrogen Receptor ◽  
Oestrous Cycle ◽  
Oestrogen Receptors ◽  
Rat Uterus ◽  
Immature Rat ◽  
High Affinity ◽  
Receptor Component ◽  
Cyclic Changes

The nuclear oestrogen receptor population in the rat uterus contained an unoccupied receptor component that bound oestradiol with the high affinity (Kd congruent to 0.5 nM) characteristic of oestrogen receptors. This unoccupied receptor was present at all phases of the oestrous cycle. Its content changed in parallel with that of the total nuclear receptor during the cycle. Oestradiol administration to the immature rat resulted in increases in the uterine content of long-term nuclear receptors (i.e., those still present 8 h after administration); these increases were due to occupied oestrogen receptors, since the content of unoccupied receptor was unchanged. Our previous experiments [White & Lim (1980) Biochem. J. 190, 833-837] have shown in contrast, that oestradiol administration results in an increase in the content of unoccupied nuclear receptor in the hypothalamus. However, as in the uterus, similar cyclic changes in the content of unoccupied nuclear receptor occurred in parallel with those of the total nuclear receptor population in the hypothalamus. Differences and similarities between the unoccupied nuclear receptor of the uterus and hypothalamus are briefly discussed.

Oestrogen receptors in endometrial cytosol of gilts on days 10-13 of oestrous cycle and pregnancy

1994 ◽  
Vol 42 (3) ◽  
pp. 217-223
Author(s):  
J. Van Der Meulen ◽  
F.A. Helmond ◽  
C.P.J. Oudenaarden
Keyword(s):  
Oestrous Cycle ◽  
Endometrial Tissue ◽  
Scatchard Plot ◽  
Oestrogen Receptors ◽  
Reproductive State ◽  
Dextran Coated Charcoal ◽  
Plot Analysis ◽  
Scatchard Plot Analysis

Cytosolic oestrogen receptors (ER(c)) were determined with a Dextran-coated charcoal assay and Scatchard plot analysis in endometrial tissue of non-pregnant and pregnant gilts on Days 10-13 after standing oestrus. The Kd of the ER(c) was 0.40 +/- 0.04 nM (mean +/- SEM) and was not affected by day or reproductive state. The endometrial ER(c) concentration was affected by day and reproductive state (P

Expression of membrane progestin receptors (mPRs) α, β and γ in the bovine uterus during the oestrous cycle and pregnancy

2019 ◽  
Vol 140 ◽  
pp. 171-179 ◽  
Author(s):  
Magdalena K. Kowalik ◽  
Karolina Dobrzyn ◽  
Robert Rekawiecki ◽  
Jan Kotwica
Keyword(s):  
Oestrous Cycle ◽  
Progestin Receptors
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