scholarly journals Determination of some biochemical and structural features of alcohol dehydrogenases from Drosophila simulans and Drosophila virilis. Comparisons of their properties with the Drosophila melanogaster Adhs enzyme

1981 ◽  
Vol 195 (3) ◽  
pp. 777-777
Keyword(s):  
Drosophila Melanogaster ◽  
Structural Features ◽  
Drosophila Simulans ◽  
Drosophila Virilis ◽  
Alcohol Dehydrogenases

scholarly journals Determination of some biochemical and structural features of alcohol dehydrogenases from Drosophila simulans and Drosophila virilis. Comparison of their properties with the Drosophila melanogaster Adhs enzyme

1981 ◽  
Vol 195 (1) ◽  
pp. 61-69 ◽  
Author(s):  
E Juan ◽  
R González-Duarte
Keyword(s):  
Drosophila Melanogaster ◽  
Amino Acid ◽  
Alcohol Dehydrogenase ◽  
Structural Features ◽  
Drosophila Simulans ◽  
Drosophila Virilis ◽  
Primary Alcohols ◽  
Terminal Amino Acid ◽  
Alcohol Dehydrogenases ◽  
Terminal Amino

The biochemical properties of the enzyme alcohol dehydrogenase of two different Drosophila species, Drosophila simulans and Drosophila virilis, were studied and compared with those of Drosophila melanogaster Adhs enzyme. All of them consist of two identical subunits of molecular weight 27800 and share significant similarities in function. The substrate specificities of these enzymes were characterized and Km(app.) and Vmax.(app.) values were calculated. All these alcohol dehydrogenases show greater affinity for secondary rather than for primary alcohols. The amino acid compositions of the three enzymes were determined, and there is a close similarity between the D. simulans and the D. melanogaster enzymes, but there are significant differences from the alcohol dehydrogenase of D. virilis. The N-terminal amino acid is blocked and the C-terminal amino acid is the same for all three alcohol dehydrogenases. The enzymes from the three species were carboxymethylated and digested with trypsin. The peptide ‘maps’ reveal, as expected, more homologies between the enzymes of D. simulans and D. melanogaster than with the enzyme of D. virilis.

scholarly journals Purification and enzyme stability of alcohol dehydrogenase from Drosophila simulans, Drosophila virilis and Drosophila melanogaster adhS

1980 ◽  
Vol 189 (1) ◽  
pp. 105-110 ◽  
Author(s):  
E Juan ◽  
R González-Duarte
Keyword(s):  
Drosophila Melanogaster ◽  
Affinity Chromatography ◽  
Enzyme Stability ◽  
Gel Filtration ◽  
Drosophila Simulans ◽  
Drosophila Virilis ◽  
Purification Procedure ◽  
Alcohol Dehydrogenases ◽  
Isoelectric Points ◽  
Chromatography Step

Three alcohol dehydrogenases from Drosophila simulans, Drosophila virillis and Drosophila melanogaster adhS (which possesses an alloenzyme with slow electrophoretic mobility) were purified essentially to homogeneity. The purification procedure involves a new step of affinity chromatography, which efficiently lowers the amount of contaminants in the final preparation, producing a very stable enzyme. The purification procedure developed consists of a salmine sulphate precipitation, two CM-Sepharose CL-6B colume-chromatography steps, an affinity-chromatography step and a Sephacryl gel filtration. A minimum of 30-fold purification is obtained and the yield is not less than 34%. The isoelectric points and molar absorption coefficients were determined.

Comparison of the neuralized genes of Drosophila virilis and D. melanogaster

Genome ◽  
1994 ◽  
Vol 37 (5) ◽  
pp. 840-847 ◽  
Author(s):  
Lily Zhou ◽  
Gabrielle L. Boulianne
Keyword(s):  
Drosophila Melanogaster ◽  
Cell Fate ◽  
Structural Similarity ◽  
Drosophila Virilis ◽  
Interspecific Comparison ◽  
Functional Regions ◽  
Embryonic Nervous System ◽  
Localization Signal ◽  
Evolutionary Comparison

The neurogenic gene neuralized of Drosophila melanogaster is thought to play a role in the determination of cell fate in the embryonic nervous system as well as other tissues during development. To determine which sequences within the neuralized gene encode functionally important domains, we have initiated an interspecific comparison of the neuralized gene of D. virilis and D. melanogaster. In this study we show that several motifs that we have previously identified in the neuralized protein of D. melanogaster are conserved in D. virilis. These include a putative nuclear localization signal, a homeodomain similarity region, and a zinc finger motif. In contrast, a helix-turn-helix motif with structural similarity to those identified as DNA-binding regions of bacterial repressors is deleted. These results demonstrate that it is possible to identify key functional regions of the neuralized protein by an interspecific comparison.Key words: Drosophila melanogaster, Drosophila virilis, neuralized, nucleotide sequence, evolutionary comparison.

DETERMINATION OF THE TYPE OF A SINGLE-USE GRENADE LAUNCHER BASED ON ITS COMPOSITE PARTS AND FRAGMENTS OF REACTIVE GRENADE FOUND AT THE PLACE OF ACCIDENT

2017 ◽  
Vol 17 ◽  
pp. 245-252
Author(s):  
V. V. Somov
Keyword(s):  
Combustion Chamber ◽  
Structural Features ◽  
Outlet Section ◽  
Technical Expertise ◽  
Jet Engine ◽  
Size Ofthe ◽  
Single Use ◽  
Nozzle Block ◽  
Considerable Damage

In carrying out an investigation into the explosion, among others, the investigative version of the use of a single-use reactive grenade launcher is being considered. The most common for criminal explosions are applied grenade launchers RPG-18, RPG-22, RPG-26. Their use is due to a number of such properties as small size and weight, which makes it possible to transfer them covertly, the range of the shot significantly exceeding the range of the hand grenade throw, the high detonating effect of the rocket grenade explosion. The single-use rocket launchers are generally of the same design. Their differences are in the features of the components construction and dimensional characteristics, which are given in the article. On the basis of expert practice, details ofgrenade launchers that remain at the site of the explosion and have the least damage are determined. These details are the objects of investigation of the explosion technical expertise. These objects include launchers of grenade launchers and rocket parts ofjet grenades. The design features of the launchers, their dimensional characteristics and marking symbols make it possible to determine their belonging to a specific type of jet grenade launchers. Missile parts of jet grenades differ in the form of the combustion chamber of the jet engine, nozzle, in the size ofthe outlet section of the nozzle, in the form and size of the stabilizerfeathers. To determine the belonging of the rocket part of the grenade to a specific type ofjet grenade launcher, it’s necessary to establish a set of structural features and dimensional characteristics. At considerable damage of the combustion chamber of the jet engine, as a rule, the nozzle block remains intact that allows to define diameter of critical section of a nozzle, and on it to establish type of the used single-use grenade launcher.

scholarly journals PSEUDOTUMORS AND FITNESS IN DROSOPHILA MELANOGASTER AND DROSOPHILA SIMULANS

Genetics ◽  
1961 ◽  
Vol 46 (8) ◽  
pp. 971-981
Author(s):  
A Di Pasquale ◽  
S Koref-Santibaňez
Keyword(s):  
Drosophila Melanogaster ◽  
Drosophila Simulans

scholarly journals Introgression of Drosophila simulans Nuclear Pore Protein 160 in Drosophila melanogaster Alone Does Not Cause Inviability but Does Cause Female Sterility

Genetics ◽  
2010 ◽  
Vol 186 (2) ◽  
pp. 669-676 ◽  
Author(s):  
Kyoichi Sawamura ◽  
Kazunori Maehara ◽  
Shotaro Mashino ◽  
Tatsuo Kagesawa ◽  
Miyuki Kajiwara ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Drosophila Simulans ◽  
Female Sterility ◽  
Nuclear Pore ◽  
Nuclear Pore Protein ◽  
Pore Protein

scholarly journals Dispersive evaluation and surface chemistry of advanced, multifunctional silica/lignin hybrid biomaterials

2013 ◽  
Vol 11 (11) ◽  
pp. 1860-1873 ◽  
Author(s):  
Magdalena Nowacka ◽  
Łukasz Klapiszewski ◽  
Małgorzata Norman ◽  
Teofil Jesionowski
Keyword(s):  
Zeta Potential ◽  
Surface Charge ◽  
Electrochemical Sensors ◽  
Relevant Information ◽  
Structural Features ◽  
Kraft Lignin ◽  
Morphological Properties ◽  
Wide Range ◽  
Hybrid Biomaterials

AbstractAdvanced silica/lignin hybrid biomaterials were obtained using hydrated or fumed silicas (Aerosil®200) and Kraft lignin as precursors, which is a cheap and biodegradable natural polymer. To extend the possible range of applications, the silicas were first modified with N-2-(aminoethyl)-3-aminopropyltrimethoxsysilane, and then with Kraft lignin, which had been oxidized with sodium periodate. The SiO2/lignin hybrids and precursors were characterised by means of determination of their physicochemical and dispersive-morphological properties. The effectiveness of silica binding to lignin was verified by FT-IR spectroscopy. The zeta potential value provides relevant information regarding interactions between colloid particles. Measurement of the zeta potential values enabled an indirect assessment of stability for the studied hybrid systems. Determination of zeta potential and density of surface charge also permitted the quantitative analysis of changes in surface charge, and indirectly confirmed the effectiveness of the proposed method for synthesis of SiO2/lignin hybrid materials. A particularly attractive feature for practical use is their stability, especially electrokinetic stability. It is expected that silica/lignin hybrids will find a wide range of applications (polymer fillers, biosorbents, electrochemical sensors), as they combine the unique properties of silica with the specific structural features of lignin. This makes these hybrids biomaterials advanced and multifunctional.

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