scholarly journals Dissociation of tumour-promoter-induced effects on prostaglandin release, polyamine synthesis and cell proliferation of 3T3 cells

1981 ◽  
Vol 194 (3) ◽  
pp. 975-982 ◽  
Author(s):  
R Lanz ◽  
K Brune
Keyword(s):  
Cell Proliferation ◽  
Ornithine Decarboxylase ◽  
Thymidine Incorporation ◽  
Decarboxylase Activity ◽  
3T3 Cells ◽  
Polyamine Synthesis ◽  
Ornithine Decarboxylase Activity ◽  
Proliferative Effect ◽  
Prostaglandin Release ◽  
Anti Inflammatory

The phorbol ester 12-O-tetradecanoylphorbol 13-acetate induces tumour promotion, inflammation, cell proliferation and prostaglandin release. Recent reports suggest that the prostaglandins released by 12-O-tetradecanoylphorbol 13-acetate (TPA) initiate a cascade of events leading to polyamine synthesis and cell proliferation. In experiments designed to test this contention, it was found that addition of TPA (1 microM to 1 nM) to confluent mouse 3T3 fibroblasts successively caused the release of prostaglandins E2 and I2, induction of the enzyme ornithine decarboxylase (EC 4.1.1.17), stimulation of [3H]thymidine incorporation into DNA, and cell proliferation. Pretreatment of the cells with the anti-inflammatory steroid dexamethasone (1 microM) or the non-steroidal anti-inflammatory drug indomethacin (1 microM) inhibited TPA-induced prostaglandin release. However, dexamethasone enhanced the other effects of TPA, whereas indomethacin was ineffective. Addition of prostaglandin E2 to the cultures did not induce ornithine decarboxylase activity and cell proliferation. Pretreatment of the cells with 1,3-diaminopropane (1 mM) or alpha-methylornithine (5 mM), inhibitors of polyamine synthesis, decreased TPA-induced ornithine decarboxylase activity without affecting DNA synthesis. TPA stimulated [3H]thymidine incorporation into DNA, even when the ornithine decarboxylase activity was completely blocked. These data suggest that the proliferative effect of TPA on 3T3 cells is independent of prostaglandin release and polyamine synthesis.

scholarly journals Effects of diamines on ornithine decarboxylase activity in control and virally transformed mouse fibroblasts

1979 ◽  
Vol 180 (1) ◽  
pp. 87-94 ◽  
Author(s):  
D R Bethell ◽  
A E Pegg
Keyword(s):  
Cell Line ◽  
Ornithine Decarboxylase ◽  
Mammalian Cells ◽  
Decarboxylase Activity ◽  
3T3 Cells ◽  
Polyamine Synthesis ◽  
Ornithine Decarboxylase Activity ◽  
3T3 Fibroblasts ◽  
Naturally Occurring ◽  
Alpha Omega

1. The induction of ornithine decarboxylase activity in mouse 3T3 fibroblasts or an SV-40 transformed 3T3 cell line by serum was prevented by addition of the naturally occurring polyamines putrescine (butane-1,4-diamine) and spermidine. Much higher concentrations of these amines were required to fully suppress ornithine decarboxylase activity in the transformed SV-3T3 cells than in the 3T3 fibroblasts. 2. Synthetic alpha omega-diamines with 3–12 carbon atoms also prevented the increase in ornithine decarboxylase activity induced by serum in these cells. The longer chain diamines were somewhat more potent than propane-1,3-diamine in this effect, but the synthetic diamines were less active than putrescine in the 3T3 cells. There was little difference between the responses of 3T3 and SV-3T3 cells to the synthetic diamines propane-1,3-diamine and heptane-1,7-diamine. 3. These results are discussed in relation to the control of polyamine synthesis in mammalian cells.

scholarly journals The effects of 24R,25-dihydroxycholecalciferol and of 1 α,25-dihydroxycholecalciferol on ornithine decarboxylase activity and on DNA synthesis in the epiphysis and diaphysis of rat bone and in the duodenum

1983 ◽  
Vol 214 (2) ◽  
pp. 293-298 ◽  
Author(s):  
D Sömjen ◽  
I Binderman ◽  
Y Weisman
Keyword(s):  
Vitamin D ◽  
Enzyme Activity ◽  
Ornithine Decarboxylase ◽  
Thymidine Incorporation ◽  
Single Injection ◽  
Fold Increase ◽  
Decarboxylase Activity ◽  
Long Bones ◽  
Vitamin D Metabolites ◽  
Ornithine Decarboxylase Activity

The effect of cholecalciferol metabolites on ornithine decarboxylase activity and on DNA synthesis in developing long bones was investigated in vitamin D-depleted rats. In the epiphysis there was a 6.4-fold increase in ornithine decarboxylase activity 5 h after a single injection of 24R,25-dihydroxycholecalciferol but not of 24S,25-dihydroxycholecalciferol or other vitamin D metabolites. In comparison, in the diaphysis and duodenum, 1 alpha,25-dihydroxycholecalciferol, but not other vitamin D metabolites, caused a 3-3.5-fold increase in the enzyme activity. The enzyme activity in the tissues examined attained a maximal value at 5 h after the injection of the metabolites. The activity of ornithine decarboxylase in the epiphysial region increased dose-dependently as the result of a single injection of 24R,25-dihydroxycholecalciferol and attained a maximal value at a dose between 30 and 3000 ng. In addition, administration of 24R,25-dihydroxycholecalciferol, but not 24S,25-dihydroxycholecalciferol or other metabolites, caused within 24 h a 1.7-2.0-fold increase in [3H]thymidine incorporation into DNA of the epiphyses of tibial bones. In comparison, 1 alpha,25-dihydroxycholecalciferol caused a 1.5-fold increase in [3H]thymidine incorporation into DNA of the diaphyses and of the duodenum. The present data indicate that 24R,25-dihydroxycholecalciferol is involved in the regulation of epiphyseal growth, whereas 1 alpha,25,dihydroxycholecalciferol stimulates the proliferation of cells in the diaphysis of long bones and in the intestinal mucosa.

Platelet derived growth factor induces ornithine decarboxylase activity in nih 3T3 cells

1985 ◽  
Vol 127 (3) ◽  
pp. 843-848 ◽  
Author(s):  
Elisa Vicenzi ◽  
Marina Bianchi ◽  
Mario Salmona ◽  
Maria Benedetta Donati ◽  
Andreina Poggi ◽  
...  
Keyword(s):  
Growth Factor ◽  
Ornithine Decarboxylase ◽  
Platelet Derived Growth Factor ◽  
Decarboxylase Activity ◽  
3T3 Cells ◽  
Ornithine Decarboxylase Activity ◽  
Nih 3T3 ◽  
Nih 3T3 Cells

scholarly journals Deoxyribonucleic acid and polyamine synthesis in rat ventral prostate. Effects of age of the intact rat and androgen stimulation of the castrated rat with testosterone, 5α-dihydrotestosterone and 5α-androstane-3β, 17β-diol

1977 ◽  
Vol 162 (1) ◽  
pp. 87-97 ◽  
Author(s):  
E E K Takyi ◽  
D J M Fuller ◽  
L J Donaldson ◽  
G H Thomas
Keyword(s):  
Ornithine Decarboxylase ◽  
Ventral Prostate ◽  
Molar Ratio ◽  
Synthetic Activity ◽  
Decarboxylase Activity ◽  
Polyamine Synthesis ◽  
Molar Ratios ◽  
Ornithine Decarboxylase Activity ◽  
The Relationship

The relationship between polyamine synthesis, growth and secretion in vivo was examined in ventral prostates from: (a) intact rats aged 3-60 weeks; (b) animals castrated for 7 days before injection with 5 alpha-dihydrotestosterone (17 beta-hydroxy-5-alpha-androstan-3-one), testosterone and 5 alpha-androstane-3 beta, 17 beta-diol for up to 10 days; (c) rats injected with the 3 beta, 17 beta-diol immediately after castration. Ornithine decarboxylase activity and the concentrations of putrescine, spermidine and spermine were measured. DNA-synthetic activity was monitored by measuring [125I]iododoxyuridine incorporation. An enhanced spermidine/spermine molar ratio reflected increased activity of the prostate. The ratio was higher (greater than 2) in prostates from sexually immature animals, than in the intact adult (1.5), suggesting that the ratio was indicative of the proliferative activity of the tissue. However, in the androgen-stimulated castrated rat, enhanced spermidine/spermine ratios tended to correlate with hypertrophy and secretion. In both sets of experiments there was a linear relationship between protein and spermidine content. High spermidine/spermine molar ratios were the consequence of a relatively low rate of accumulation of spermine relative to spermidine and protein. The relationship between polyamine synthesis and DNA-synthetic activity was investigated in cultured prostate. A combination of insulin (3 mug/ml) and testosterone (0.1 muM caused a stimulatory response in the incorporation of [125I]iododeoxyuridine and in cell division, despite a depleted polyamine content and low ornithine decarboxylase activity in the cultured tissue.

scholarly journals Polyamine synthesis in bone marrow granulocytes: effect of cell maturity and early changes following an inflammatory stimulus

Blood ◽  
1978 ◽  
Vol 51 (6) ◽  
pp. 1021-1029
Author(s):  
WH Evans ◽  
CK Grieshaber ◽  
WC Miller ◽  
SM Wilson ◽  
HA Hoffman
Keyword(s):  
Bone Marrow ◽  
Ornithine Decarboxylase ◽  
Synthetic Activity ◽  
Neutrophil Granulocytes ◽  
Decarboxylase Activity ◽  
Inflammatory Stimulus ◽  
Maximum Increase ◽  
Polyamine Synthesis ◽  
Ornithine Decarboxylase Activity ◽  
Bone Marrow Granulocytes

Enriched fractions of mature and immature neutrophil granulocytes, isolated from guinea pig bone marrow, were assayed for ornithine decarboxylase activity and polyamine content. The results show that immature granulocytes contain at least ten times more ornithine decarboxylase activity and two times more spermidine than mature granulocytes. The incorporation of 14C-ornithine into putrescine and spermidine of intact immature granulocytes was three to four times and ten times, respectively, that of mature granulocyte preparations. Six hours after an inflammatory stimulus, transient increases of 14-fold and 3-fold in the activities of ornithine decarboxylase and S-adenosyl- L-methionine decarboxylase, respectively, were observed in immature bone marrow granulocytes. At this time the incorporation of 14C- ornithine into putrescine and spermidine in bone marrow granulocytes from stimulated animals was 14 times that of cells from controls. A maximum increase in DNA synthesis in these cells during the inflammatory response occurred 6 hr after the maximum increase in the polyamine synthetic activity. Together these data suggest that polyamine synthesis in the granulocyte compartment of the bone marrow is associated chiefly with immature proliferating cells and that increased polyamine synthesis precedes increased granulocyte proliferation in the bone marrow following an inflammatory stimulus.

scholarly journals Polyamine synthesis in bone marrow granulocytes: effect of cell maturity and early changes following an inflammatory stimulus

Blood ◽  
1978 ◽  
Vol 51 (6) ◽  
pp. 1021-1029 ◽  
Author(s):  
WH Evans ◽  
CK Grieshaber ◽  
WC Miller ◽  
SM Wilson ◽  
HA Hoffman
Keyword(s):  
Bone Marrow ◽  
Ornithine Decarboxylase ◽  
Synthetic Activity ◽  
Neutrophil Granulocytes ◽  
Decarboxylase Activity ◽  
Inflammatory Stimulus ◽  
Maximum Increase ◽  
Polyamine Synthesis ◽  
Ornithine Decarboxylase Activity ◽  
Bone Marrow Granulocytes

Abstract Enriched fractions of mature and immature neutrophil granulocytes, isolated from guinea pig bone marrow, were assayed for ornithine decarboxylase activity and polyamine content. The results show that immature granulocytes contain at least ten times more ornithine decarboxylase activity and two times more spermidine than mature granulocytes. The incorporation of 14C-ornithine into putrescine and spermidine of intact immature granulocytes was three to four times and ten times, respectively, that of mature granulocyte preparations. Six hours after an inflammatory stimulus, transient increases of 14-fold and 3-fold in the activities of ornithine decarboxylase and S-adenosyl- L-methionine decarboxylase, respectively, were observed in immature bone marrow granulocytes. At this time the incorporation of 14C- ornithine into putrescine and spermidine in bone marrow granulocytes from stimulated animals was 14 times that of cells from controls. A maximum increase in DNA synthesis in these cells during the inflammatory response occurred 6 hr after the maximum increase in the polyamine synthetic activity. Together these data suggest that polyamine synthesis in the granulocyte compartment of the bone marrow is associated chiefly with immature proliferating cells and that increased polyamine synthesis precedes increased granulocyte proliferation in the bone marrow following an inflammatory stimulus.

Sign in / Sign up

Export Citation Format

Share Document