scholarly journals Subcellular localization of low-molecular-weight RNA components in rat liver

1981 ◽  
Vol 193 (3) ◽  
pp. 743-748 ◽  
Author(s):  
S Frederiksen ◽  
H Flodgard ◽  
P Hellung-Larsen
Keyword(s):  
Molecular Weight ◽  
Subcellular Localization ◽  
Rat Liver ◽  
Liver Cells ◽  
Low Molecular Weight ◽  
Cytoplasmic Fraction ◽  
Rat Liver Cells ◽  
Component C

The subcellular localization of the four major low-molecular-weight RNA components, D, C, A and L, was studied in rat liver cells. The cells were fractionated by a non-aqueous technique into a nuclear and a cytoplasmic fraction. The cytoplasm contained 43% of component D, 57% of component C and more than 80% of component L.

Studies on the template activity of ‘isolated’ Xenopus erythrocyte nuclei. II. The effects of cytoplasmic extracts

1977 ◽  
Vol 24 (1) ◽  
pp. 119-129
Author(s):  
N. Maclean ◽  
V.A. Hilder
Keyword(s):  
Molecular Weight ◽  
Genetic Control ◽  
Rat Liver ◽  
Blood Cells ◽  
Rna Synthesis ◽  
Liver Cells ◽  
Template Activity ◽  
Rat Liver Cells

The effects of exposing nuclei isolated from Xenopus erythrocytes to cytoplasmic extracts prepared from various sources on their template activity are described. The cytoplasm of rat liver cells and the immature blood cells of Xenopus contain factors which stimulate RNA synthesis in these essentially inactive nuclei, whereas the cytoplasm of many other cells does not yield such factors. The active factors in rat liver cytoplasmic extracts appear to be proteins of molecular weight about 40000 Daltons. The implications of these results for genetic control in eukaryotes are discussed.

Effects of Hypophysectomy on the Fine Structure of Rat Liver Cells

1967 ◽  
Vol 25 ◽  
pp. 156-157
Author(s):  
Robert R. Cardell
Keyword(s):  
Electron Microscopy ◽  
Fine Structure ◽  
Rat Liver ◽  
Liver Cell ◽  
Anterior Pituitary ◽  
Liver Cells ◽  
Biochemical Studies ◽  
Liver Functions ◽  
Rat Liver Cells ◽  
And Control

Hypophysectomy of the rat renders this animal deficient in the hormones of the anterior pituitary gland, thus causing many primary and secondary hormonal effects on basic liver functions. Biochemical studies of these alterations in the rat liver cell are quite extensive; however, relatively few morphological observations on such cells have been recorded. Because the available biochemical information was derived mostly from disrupted and fractionated liver cells, it seemed desirable to examine the problem with the techniques of electron microscopy in order to see what changes are apparent in the intact liver cell after hypophysectomy. Accordingly, liver cells from rats which had been hypophysectomized 5-120 days before sacrifice were studied. Sham-operated rats served as controls and both hypophysectomized and control rats were fasted 15 hours before sacrifice.

Heterogeneity of carboxylesterases in rat liver cells

1992 ◽  
Vol 44 (4) ◽  
pp. 827-829 ◽  
Author(s):  
Rolf Gaustad ◽  
Trond Berg ◽  
Frode Fonnum
Keyword(s):  
Rat Liver ◽  
Liver Cells ◽  
Rat Liver Cells
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