scholarly journals Fructose 2,6-bisphosphate, the probably structure of the glucose- and glucagon-sensitive stimulator of phosphofructokinase

1980 ◽  
Vol 192 (3) ◽  
pp. 897-901 ◽  
Author(s):  
E Van Schaftingen ◽  
L Hue ◽  
H G Hers
Keyword(s):  
Molecular Weight ◽  
Rat Liver ◽  
Reducing Power ◽  
Organic Phosphate ◽  
Low Molecular Weight ◽  
Acid Labile

The low-molecular-weight stimulator of phosphofructokinase [Van Schaftingen, Hue & Hers (1980) Biochem. J. 192, 887-895] has been purified from rat liver. It was completely destroyed upon incubation with 0.01 M-HCl for 10 min at 20 degrees C and fructose 6-phosphate and a reducing power equivalent in amount to the acid-labile organic phosphate were formed. It was therefore tentatively identified as fructose 2,6-bisphosphate.

Biosynthesis of the Low Molecular Weight Carrier Protein for Insulin-Like Growth Factors in Rat Liver and Fibroblasts*

Endocrinology ◽  
1987 ◽  
Vol 121 (3) ◽  
pp. 1041-1050 ◽  
Author(s):  
JOYCE A. ROMANUS ◽  
YVONNE W.-H. YANG ◽  
S. PETER NISSLEY ◽  
MATTHEW M. RECHLER
Keyword(s):  
Molecular Weight ◽  
Growth Factors ◽  
Rat Liver ◽  
Low Molecular Weight ◽  
Carrier Protein ◽  
Insulin Like Growth Factors

Acid-labile poly(amino alcohol ortho ester) based on low molecular weight polyethyleneimine for gene delivery

2017 ◽  
Vol 32 (3) ◽  
pp. 349-361 ◽  
Author(s):  
Jun Wang ◽  
Lei Zhang ◽  
Xin Wang ◽  
Shengxiang Fu ◽  
Guoqing Yan
Keyword(s):  
Molecular Weight ◽  
Amino Alcohol ◽  
Water Solubility ◽  
Transfection Efficiency ◽  
Enzymatic Digestion ◽  
Low Molecular Weight ◽  
Hydroxyl Groups ◽  
Ortho Ester ◽  
Human Neuroblastoma ◽  
Acid Labile

A series of acid-labile poly(amino alcohol ortho ester) (POEeis) were synthesized through ring-opening polymerization between diglycidyl ethers with ortho ester bonded and low molecular weight polyethyleneimine by various feed molar ratios. The obtain POEei 1 and POEei 2 exhibited clear kinetic of degradation and condensed plasmid DNA into nanoparticles of suitable sizes (250–300 nm) and positive zeta potentials (+20–30 mV) while protecting DNA from enzymatic digestion. Further, these polymers have uniform distribution of abundant hydroxyl groups, which could improve their water solubility, biocompatibility, and lower protein adsorption. Significantly, ortho ester groups in POEeis main-chains could hydrolyze rapidly at acidic endosomal pH, resulting in intracellular DNA release and diminished material toxicity. MTT assay revealed that all the polymers exhibited much lower cytotoxicity than 25 kDa PEI in the human neuroblastoma SH-SY5Y cells. Moreover, the transfection efficiency of POEei 1 was higher than 25 kDa PEI in serum-free medium or 10% serum medium.

scholarly journals Increased synthesis of low-molecular-weight nuclear ribonucleic acids of rat liver after γ irradiation and hepatectomy

1975 ◽  
Vol 146 (2) ◽  
pp. 357-360 ◽  
Author(s):  
A Fónagy ◽  
E J Hidvégi
Keyword(s):  
Molecular Weight ◽  
Partial Hepatectomy ◽  
Rat Liver ◽  
Orotic Acid ◽  
Low Molecular Weight ◽  
Polyacrylamide Gels ◽  
Γ Irradiation ◽  
Ribonucleic Acids

Incorporation of [3H]orotic acid into low-molecular-weight nRNA of rat liver, fractionated on polyacrylamide gels, increased 6-12h after partial hepatectomy and 6h after γ-irridation at 2000 R. The incorporation of orotic acid was particularly increased into the 4.5S, 5S and approx. 10S nRNA fractions. If the irradiation was given after 6h of regeneration and RNA was isolated from the nucleus 12h after hepatectomy then the incorporation of orotic acid into these low-molecular-weight nRNA components was greater than after hepatectomy or irradiation alone.

scholarly journals Two distinct low-molecular-weight acid phosphatases from rat liver.

1988 ◽  
Vol 36 (8) ◽  
pp. 3020-3026 ◽  
Author(s):  
SADAKI FUJIMOTO ◽  
KAZUKI MURAKAMI ◽  
AKIRA ISHIKAWA ◽  
KENJI HIMI ◽  
AKIRA OHARA
Keyword(s):  
Molecular Weight ◽  
Rat Liver ◽  
Low Molecular Weight ◽  
Acid Phosphatases

scholarly journals Degradation of [3H]chondroitin 4-sulphate and re-utilization of the [3H]hexosamine component by the isolated perfused rat liver

1980 ◽  
Vol 186 (1) ◽  
pp. 279-286 ◽  
Author(s):  
A D MacNicholl ◽  
F S Wusteman ◽  
P J Winterburn ◽  
G M Powell ◽  
C G Curtis
Keyword(s):  
Molecular Weight ◽  
Rat Liver ◽  
System Analysis ◽  
Isolated Perfused Rat Liver ◽  
Amino Sugars ◽  
Low Molecular Weight ◽  
Perfused Rat Liver ◽  
Proteolytic Digestion ◽  
Rib Cartilage ◽  
Molecular Weight Form

Radiolabelled chondroitin 4-sulphate was isolated after incubation of rat rib cartilage with N-acetyl-D-[6-3H]galactosamine. After proteolytic digestion of the tissue with either papain or trypsin the released [3H]chondroitin 4-sulphate was added to an isolated perfused rat liver system. Analysis of perfusate after several hours perfusion showed that radiolabelled amino sugars were secreted by the liver in a low-molecular-weight form and as components of glycoproteins.

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