scholarly journals Complexing reverse transcriptase with polyspermine-ribonuclease

1980 ◽  
Vol 189 (1) ◽  
pp. 89-93
Author(s):  
A K Bandyopadhyay ◽  
D Wang ◽  
C C Levy
Keyword(s):  
Complex Formation ◽  
Reverse Transcriptase ◽  
Reverse Transcriptase Activity ◽  
Favourable Condition ◽  
Molar Ratio ◽  
Leukaemia Virus ◽  
Transcriptase Activity

Polyspermine-ribonuclease (Mr approximately 17 000) and the enzyme transcriptase from Rauscher-leukaemia virus (Mr approximately 70 000) form a complex Mr approx. 160 000) such that the molar ratio of polyspermine-ribonuclease to reverse transcriptase is 5:1. The most favourable condition for complex-formation is in a solution consisting of 0.01 M-Tris/HCl buffer, pH 7.5, 0.25 M-KCl and 1 mM-Mn2+ at 37 degrees C. The association of the two enzymes retains full RNAase activity, but reverse-transcriptase activity is completely inhibited when ribonuclease-sensitive polymers such as (dG)12 x (rC)n or viral 70S RNA are used as primer templates.

scholarly journals Inhibition of murine leukaemia virus reverse transcriptase by 2-halogenated polyadenylic acids

1982 ◽  
Vol 203 (3) ◽  
pp. 755-760 ◽  
Author(s):  
T Fukui ◽  
E De Clercq
Keyword(s):  
Reverse Transcriptase ◽  
Dna Polymerase ◽  
Reverse Transcriptase Activity ◽  
Leukaemia Virus ◽  
Transcriptase Activity ◽  
Murine Leukaemia Virus ◽  
Polyadenylic Acid ◽  
Moloney Murine Leukaemia Virus

Several new analogues of polyadenylic acid [(A)n], i.e. poly(2-fluoroadenylic acid) [(fl2A)n], poly(2-chloroadenylic acid [(cl2A)n], poly(2-bromoadenylic acid) [(br2A)n] and poly(2-iodoadenylic acid) [(io2A)n] have been synthesized and evaluated for their effects on the RNA-directed DNA polymerase (reverse transcriptase) activity of Moloney murine leukaemia virus. All (A)n analogues were found to be potent inhibitors of reverse transcriptase, the order of (decreasing) potency being (fl2A)n greater than (io2A)n greater than (br2A)n greater than (cl2A)n. For all four (A)n analogues the inhibition of reverse transcriptase was competitive with respect to the template-primer. (A)n . oligo(dT). The K1 values were 0.02 microgram/ml for (fl2A)n, 0.1 microgram/ml for (io2A)n, 0.5 microgram/ml for (br2A)n and 8 microgram/ml for (cl2A)n. With a Ki of 0.02 microgram/ml (approx. 0.04 microM), (fl2A)n can be considered as one of the most, if not the most, potent polynucleotide inhibitor of reverse transcriptase that has been described so far.

A Method for Combined Immunoaffinity Purification and Assay of HIV-1 Reverse Transcriptase Activity Useful for Crude Samples

1996 ◽  
Vol 235 (2) ◽  
pp. 141-152 ◽  
Author(s):  
J. Lennerstrand ◽  
A.-S. Rytting ◽  
C. Örvell ◽  
J.S. Gronowitz ◽  
C.F.R. Källander
Keyword(s):  
Reverse Transcriptase ◽  
Reverse Transcriptase Activity ◽  
Transcriptase Activity ◽  
Immunoaffinity Purification ◽  
Hiv 1

scholarly journals Reverse transcriptase activity associated with haemic neoplasia in the soft-shell clam Mya arenaria

2009 ◽  
Vol 84 ◽  
pp. 57-63 ◽  
Author(s):  
M AboElkhair ◽  
A Siah ◽  
KF Clark ◽  
P McKenna ◽  
J Pariseau ◽  
...  
Keyword(s):  
Reverse Transcriptase ◽  
Reverse Transcriptase Activity ◽  
Mya Arenaria ◽  
Soft Shell Clam ◽  
Transcriptase Activity ◽  
Soft Shell
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