scholarly journals Glucose metabolism and recycling of radioactively labelled glucose in the Zucker genetically obese rat (fa/fa)

1980 ◽  
Vol 186 (1) ◽  
pp. 161-168 ◽  
Author(s):  
A J Wade
Keyword(s):  
Single Dose ◽  
Food Intake ◽  
Glucose Metabolism ◽  
Mean Transit Time ◽  
Replacement Rate ◽  
Obese Rats ◽  
The Mean ◽  
Total Body ◽  
Minimal Mass ◽  
Made In

1. The glucose metabolism of conscious lean and obese rats of the Zucker strain was studied by using doubly labelled glucose ([6-3H,U-14C]glucose) given by intravenous injection as a single dose. Fed animals were used, allowing the study to be made in conditions favouring active lipogenesis. 2. At any given prior food intake (consumption during preceding 24 h), the irreversible glucose replacement rate, R0, was considerably higher in the growing obese rat (4-6 months old) when both of these variables were scaled in terms of the total body water of the animals. 3. When scaled in a similar way, the minimal mass of glucose (Mmin.) was also larger in the obese rats. The mean transit time, t, through the pool did not differ significantly between the two groups, but there was a tendency for this to be shorter in obese rats. 4. There was no difference in the proportion of 14C (derived from metabolized labelled glucose) that recycled as [14C]-glucose after passing through the pyruvate pool in the two groups of rats if the rate of recycling of radioactivity (Rc) was expressed as a percentage of R0.

scholarly journals The effects of hypoglycin on glucose metabolism in the rat. A kinetic study in vivo and [U-14C,2-3H]glucose

1978 ◽  
Vol 170 (2) ◽  
pp. 337-342 ◽  
Author(s):  
H Osmundsen ◽  
D Billington ◽  
J R Taylor ◽  
H S A Sherratt
Keyword(s):  
Blood Glucose ◽  
Glucose Metabolism ◽  
Kinetic Study ◽  
Mean Transit Time ◽  
Intravenous Dose ◽  
Total Body Mass ◽  
The Mean ◽  
Total Body ◽  
Kinetics Of

1. The kinetics of glucose metabolism were evaluated in rats deprived of food 15-21 h after the administration of hypoglycaemic doses of hypoglycin (100 mg/kg body wt.) by following changes in the specific radioactivities of 14C and 3H in blood glucose after an intravenous dose of [U-14C,2-3H]glucose [Katz, Rostami & Dunn (1974) Biochem. J. 142, 161-170]. 2. During this time, recycling of glucose through the Cori cycle was virtually abolished, the rate of irreversible disposal of glucose and its total body mass were both decreased by about 70%, whereas there was little effect on the mean transit time for glucose. 3. It was concluded that hypoglycaemia is due to inhibition of gluconeogenesis.

scholarly journals Pharmacokinetics and Safety of Ascending Single Doses of DZ-2640, a New Oral Carbapenem Antibiotic, Administered to Healthy Japanese Subjects

2000 ◽  
Vol 44 (3) ◽  
pp. 578-582 ◽  
Author(s):  
Makoto Tanaka ◽  
Kinuyo Kato ◽  
Hideo Hakusui ◽  
Yoichi Murakami ◽  
Kenichi Sato ◽  
...  
Keyword(s):  
Single Dose ◽  
Food Intake ◽  
Half Life ◽  
High Performance ◽  
Parent Compound ◽  
Tubular Secretion ◽  
Active Metabolites ◽  
Time Curve ◽  
Chromatography Method ◽  
The Mean

ABSTRACT DZ-2640 is the ester-type oral carbapenem prodrug of an active parent compound, DU-6681. The pharmacokinetics and safety of DU-6681 were investigated in six studies after oral administration of a single dose of DZ-2640 to healthy male Japanese volunteers at doses of 25, 50, 100, 200, and 400 mg (as the equivalents of DU-6681) in the fasted state. The same volunteers received the drug at a dose of 100 mg in the fasted and fed states to examine the effect of food intake on the bioavailability of DZ-2640. The concentrations of DU-6681 in plasma and urine were determined by a validated high-performance liquid chromatography method and a bioassay. A good correlation between both methods was seen, indicating an absence of major active metabolites. The mean maximum concentrations of DU-6681 in plasma (C max) ranged from 0.263 μg/ml (25-mg dose) to 2.489 μg/ml (400-mg dose) and were reached within 1.5 h following drug administration. After reaching theC max, plasma DU-6681 concentrations declined in a monophasic manner, with a half-life of 0.47 to 0.89 h. The area under the concentration-time curve (AUC) andC max increased almost linearly with the dose up to the 200-mg dose. The AUC and C max increased less than proportionally after administration of the 400-mg dose, suggesting a reduction in drug absorption. The plasma protein binding of DU-6681 was in the range of 23.3 to 25.6%. The cumulative urinary recoveries (0 to 24 h) were in the range of 31.9 to 44.9%. The AUC was slightly but statistically significantly reduced by food intake. However, the C max, half-life, and recovery in urine were not affected by food intake. The renal clearance (402 to 510 ml/min) was much greater than the mean glomerular filtration rate (ca. 120 ml/min), which indicated active tubular secretion of the drug. A mild transient and moderate diarrhea was observed in two of six volunteers in the study with a single dose of 25 mg. Mild soft stools were observed in two of six volunteers who received a 400-mg dose of the drug.

scholarly journals Evaluation of glucose turnover, body mass and recycling with reversible and irreversible tracers

1974 ◽  
Vol 142 (1) ◽  
pp. 161-170 ◽  
Author(s):  
Joseph Katz ◽  
H. Rostami ◽  
Arnold Dunn
Keyword(s):  
Steady State ◽  
Glucose Metabolism ◽  
Single Injection ◽  
Mean Transit Time ◽  
Specific Radioactivity ◽  
Glucose Turnover ◽  
Glucose Carbon ◽  
Multicompartmental Model ◽  
Labelled Compound ◽  
Total Body

1. Methods are presented for the calculation of rates of synthesis or loss, mean transit time and total body pool of compounds from specific-radioactivity curves, without assuming a multicompartmental model and without fitting the data by exponential expressions. The methods apply to the steady state after either single injection or continuous infusion of a labelled compound. 2. The use of irreversible and reversible tracers and the effects of recycling of carbon on the estimations of the parameters of glucose metabolism are discussed. Methods for quantitatively determining recycling of glucose carbon by the use of glucose doubly labelled with 14C and 3H are presented.

Glucose utilization and recycling in ponies

1976 ◽  
Vol 230 (1) ◽  
pp. 138-142 ◽  
Author(s):  
MS Anwer ◽  
TE Chapman ◽  
R Gronwall
Keyword(s):  
Glucose Metabolism ◽  
Plasma Glucose ◽  
Glucose Utilization ◽  
Futile Cycle ◽  
Cori Cycle ◽  
Glucose Carbon ◽  
Total Body ◽  
Minimal Mass

Variables of glucose metabolism determined by the use of [U-14C]glucose were compared in fed and fasted ponies. Relative recycling of glucose carbon with respect to tritium in fed animals was negligible for 6-T and 3-T and 16% for 2-T studies; in fasted animals relative recycling was 12 and 14% for 6-T and 3-T studies, respectively. Minimal mass of total-body glucose decreased significantly in the fasted ponies. Based on relative recycling of carbon to tritium, a negligible fraction of plasma glucose was produced via the Cori cycle or from glycerol in fed ponies; recycled tricarbon units contributed 12% of glucose produciton in ponies fasted 72 h. In fed ponies, 16% of plasma glucose carbon was recycled via a futile cycle at the glucose 6-phosphate stage. Glucose utilization was best estimated with the use of [6-T]glucose (or 3-T).

Total body fat content and fat topography are associated differently with in vivo glucose metabolism in nonobese and obese nondiabetic women

Diabetes ◽  
1992 ◽  
Vol 41 (9) ◽  
pp. 1151-1159 ◽  
Author(s):  
E. Bonora ◽  
S. Del Prato ◽  
R. C. Bonadonna ◽  
G. Gulli ◽  
A. Solini ◽  
...  
Keyword(s):  
Glucose Metabolism ◽  
Body Fat ◽  
Fat Content ◽  
Body Fat Content ◽  
Total Body Fat ◽  
Total Body

scholarly journals The implications of lag times between nitrate leaching losses and riverine loads for water quality policy

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
R. W. McDowell ◽  
Z. P. Simpson ◽  
A. G. Ausseil ◽  
Z. Etheridge ◽  
R. Law
Keyword(s):  
Water Quality ◽  
Mean Transit Time ◽  
Lag Time ◽  
Practice Change ◽  
N Leaching ◽  
N Losses ◽  
Leaching Losses ◽  
Nitrate N ◽  
The Mean ◽  
Lag Times

AbstractUnderstanding the lag time between land management and impacts on riverine nitrate–nitrogen (N) loads is critical to understand when action to mitigate nitrate–N leaching losses from the soil profile may start improving water quality. These lags occur due to leaching of nitrate–N through the subsurface (soil and groundwater). Actions to mitigate nitrate–N losses have been mandated in New Zealand policy to start showing improvements in water quality within five years. We estimated annual rates of nitrate–N leaching and annual nitrate–N loads for 77 river catchments from 1990 to 2018. Lag times between these losses and riverine loads were determined for 34 catchments but could not be determined in other catchments because they exhibited little change in nitrate–N leaching losses or loads. Lag times varied from 1 to 12 years according to factors like catchment size (Strahler stream order and altitude) and slope. For eight catchments where additional isotope and modelling data were available, the mean transit time for surface water at baseflow to pass through the catchment was on average 2.1 years less than, and never greater than, the mean lag time for nitrate–N, inferring our lag time estimates were robust. The median lag time for nitrate–N across the 34 catchments was 4.5 years, meaning that nearly half of these catchments wouldn’t exhibit decreases in nitrate–N because of practice change within the five years outlined in policy.

60 Contact Hand Burns in Children: Still a Major Prevention Need

2020 ◽  
Vol 41 (Supplement_1) ◽  
pp. S39-S40
Author(s):  
Jaclyn M McBride ◽  
Kathleen S Romanowski ◽  
Soman Sen ◽  
Tina L Palmieri ◽  
David G Greenhalgh
Keyword(s):  
Graft Loss ◽  
Dorsal Surface ◽  
Skin Grafting ◽  
Secondary Surgery ◽  
Surface Areas ◽  
Palmar Surface ◽  
The Mean ◽  
Total Body ◽  
Hand Burns ◽  
Palmar Aspect

Abstract Introduction Since toddlers explore with their hands, contact burns continue to be a major pediatric problem. The purpose of this report is to review a pediatric burn unit’s 8-year experience with contact burns of the hand. Methods After IRB approval, a review of pediatric contact hand burns that occurred between 2006–2014 was performed. We examined the causes and outcomes in pediatric contact hand burns in a single pediatric burn program. Results In the 8-year span, 535 children suffered contact burns to the hand (67 per year). The majority suffered hands burns from an oven or stove (120). The other etiologies included burns from a fireplace (76), clothing iron (65), curling or straightening iron (50), and firepit or campfire (46). The mean age at time of injury was 2.62 years old, with a range of 2 months old to18 years old. Male children (339) typically burned their hands more than females (197). Locations of injury included the palmar surface, dorsal surface, fingers tips/thumb, wrist or a combination of several different areas. Most children burned the palmar aspect of their hand (384) compared to the dorsal aspect (61). These burns typically cover small total body surface areas (mean 1.08% TBSA), with only 2% of burns comprising >5% TBSA. Approximately, 84% of these patients did not need surgery, but 86 (16%) had skin grafting (usually full-thickness) and 26% needed a secondary surgery. Of those that needed more than two, the average number of procedures was 3.6. Approximately 4.1% of patients needed a tertiary surgery. Causes for tertiary surgeries included contractures and graft loss. Out of twenty-two patients that needed a third surgery, 59% were due to graft loss and 41% were due to contractures. Conclusions Contact burns to the hand continue to be a major problem for toddlers. Children are most likely to burn themselves on an oven or stove, fireplace, clothing iron or curling/straightening iron. The palmar surface of the hand is the most likely site. While most children do not require surgery, approximately 16% require grafting. A significant number of those patients need reconstructive surgery. Clearly, current prevention efforts have failed to reduce these injuries. Applicability of Research to Practice Palm burns are common in young children. Efforts should focus on preventing these injuries.

Central glucagon-like peptide 1 receptor-induced anorexia requires glucose metabolism-mediated suppression of AMPK and is impaired by central fructose

2013 ◽  
Vol 304 (7) ◽  
pp. E677-E685 ◽  
Author(s):  
Melissa A. Burmeister ◽  
Jennifer Ayala ◽  
Daniel J. Drucker ◽  
Julio E. Ayala
Keyword(s):  
Food Intake ◽  
Glucose Metabolism ◽  
Dependent Manner ◽  
Anorectic Effect ◽  
Dependent Inhibition ◽  
Glycolytic Inhibitor ◽  
Glucagon Like Peptide ◽  
Glucagon Like Peptide 1 ◽  
Amp Activated Protein Kinase ◽  
Mediated Reduction

Glucagon-like peptide-1 (GLP-1) suppresses food intake via activation of a central (i.e., brain) GLP-1 receptor (GLP-1R). Central AMP-activated protein kinase (AMPK) is a nutrient-sensitive regulator of food intake that is inhibited by anorectic signals. The anorectic effect elicited by hindbrain GLP-1R activation is attenuated by the AMPK stimulator AICAR. This suggests that central GLP-1R activation suppresses food intake via inhibition of central AMPK. The present studies examined the mechanism(s) by which central GLP-1R activation inhibits AMPK. Supporting previous findings, AICAR attenuated the anorectic effect elicited by intracerebroventricular (icv) administration of the GLP-1R agonist exendin-4 (Ex-4). We demonstrate that Ex-4 stimulates glycolysis and suppresses AMPK phosphorylation in a glucose-dependent manner in hypothalamic GT1-7 cells. This suggests that inhibition of AMPK and food intake by Ex-4 requires central glucose metabolism. Supporting this, the glycolytic inhibitor 2-deoxyglucose (2-DG) attenuated the anorectic effect of Ex-4. However, icv glucose did not enhance the suppression of food intake by Ex-4. AICAR had no effect on Ex-4-mediated reduction in locomotor activity. We also tested whether other carbohydrates affect the anorectic response to Ex-4. Intracerebroventricular pretreatment with the sucrose metabolite fructose, an AMPK activator, attenuated the anorectic effect of Ex-4. This potentially explains the increased food intake observed in sucrose-fed mice. In summary, we propose a model whereby activation of the central GLP-1R reduces food intake via glucose metabolism-dependent inhibition of central AMPK. We also suggest that fructose stimulates food intake by impairing central GLP-1R action. This has significant implications given the correlation between sugar consumption and obesity.

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