scholarly journals Translational activity of messenger ribonucleic acid isolated from unstimulated and phytohaemagglutinin-activated lymphocytes

1979 ◽  
Vol 184 (2) ◽  
pp. 277-282 ◽  
Author(s):  
D M Wallace ◽  
R Jagus ◽  
C R Benzie ◽  
J E Kay
Keyword(s):  
Protein Synthesis ◽  
Ribonucleic Acid ◽  
Wheat Germ ◽  
Lower Efficiency ◽  
Free System ◽  
Activated Lymphocytes ◽  
Messenger Ribonucleic Acid ◽  
Translational Activity ◽  
Routine Assessment ◽  
Degree Of Purification

Purified cytoplasmic poly(A)+ RNA isolated from unstimulated pig lymphocytes has the same ability to direct translation in a range of cell-free systems as the corresponding mRNA from 20h phytohaemagglutinin-activated lymphocytes. Additional methylation of the mRNA is not required for maximum protein synthesis in the wheat-germ cell-free system. Misleading results are obtained if the mRNA preparations used are not adequately purified, and a method suitable for routine assessment of the degree of purification achieved is described. Cell-free protein-synthesizing systems from unstimulated lymphocytes translate added lymphocyte mRNA with lower efficiency than do comparable systems from phytohaemagglutinin-activated lymphocytes, whatever the source of the mRNA used.

Mechanism of action of an inhibitor from Proteus vulgaris on cell-free protein synthesis by Escherichia coli B

1969 ◽  
Vol 15 (2) ◽  
pp. 159-164
Author(s):  
J. J. McEvoy ◽  
W. E. Inniss
Keyword(s):  
Escherichia Coli ◽  
Amino Acids ◽  
Protein Synthesis ◽  
Mechanism Of Action ◽  
Ribonucleic Acid ◽  
Proteus Vulgaris ◽  
Flash Evaporation ◽  
Free System ◽  
Messenger Ribonucleic Acid ◽  
Escherichia Coli B

An inhibitory substance(s) has been found in S-30 fractions from Proteus vulgaris which prevented an Escherichia coli B cell-free system from incorporating a mixture of 14C-amino acids, L-phenylalanine-14C, or L-lysine-14C into protein, as directed by natural messenger ribonucleic acid, polyuridylic acid, or polyadenylic acid respectively. Similar results were obtained when the inhibitor was isolated from S-100 fractions by using dialysis, concentration of the dialysate by flash evaporation, hydrolysis, evaporation to dryness, dissolution to the original volume in distilled water, and neutralization. The effect of the inhibitor on the various individual reactions involved in protein synthesis was examined. No effect was found on the activation of amino acids as determined by the formation of L-phenylalanine-14C hydroxamate isolated chromatographically or by adenosine triphosphate – pyrophosphate exchange. Also no inhibition of L-phenylalanine-14C attachment to transfer ribonucleic acid occurred. However, ribosome-dependent reactions were markedly inhibited. The mechanism of action of the inhibitor appeared to be the prevention of binding of phenylalanyl-transfer ribonucleic acid to the ribosomes.

Effects of ribosomal wash factors and spermidine on endogenous and exogenous mRNA stimulated protein synthesis in the wheat germ cell-free system

1979 ◽  
Vol 57 (5) ◽  
pp. 429-435
Author(s):  
Elahe Elahi ◽  
George H. Jones
Keyword(s):  
Protein Synthesis ◽  
Germ Cell ◽  
Wheat Germ ◽  
Mrna Translation ◽  
The Other ◽  
Globin Mrna ◽  
Free System ◽  
Differential Effects ◽  
Myeloma Proteins ◽  
Dependent Protein

Differential effects of Mg2+, spermidine, and reticulocyte ribosomal wash factors on the translation of endogenous, myeloma, and globin mRNA's have been observed in studies with the wheat germ cell-free protein synthesizing system. Spermidine stimulated globin mRNA translation but not the translation of endogenous wheat germ messages, and the polyamine actually inhibited the translation of myeloma mRNA. Ribosomal wash factors, on the other hand, stimulated endogenous and myeloma mRNA dependent protein synthesis in an Mg2+-dependent fashion but inhibited globin mRNA translation. The combination of ribosomal wash factors and spermidine was either stimulatory or inhibitory depending on the Mg2+ concentration and the message. It was further observed that translation of exogenous myeloma mRNA proceeded for only 60 min at 25 °C under all conditions tested in this study, while translation of endogenous wheat germ messages continued for longer periods of time. No differential effects of spermidine on the synthesis of high molecular weight myeloma proteins were observed.

Ribosomal Aggregates Engaged in Protein Synthesis : Ergosome Breakdown and Messenger Ribonucleic Acid Transport

Nature ◽  
1963 ◽  
Vol 198 (4881) ◽  
pp. 632-638 ◽  
Author(s):  
HANS NOLL ◽  
THEOPHIL STAEHELIN ◽  
F. O. WETTSTEIN
Keyword(s):  
Protein Synthesis ◽  
Ribonucleic Acid ◽  
Acid Transport ◽  
Messenger Ribonucleic Acid
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