scholarly journals The asymmetric distribution of enzymic activity between the six subunits of bovine liver glutamate dehydrogenase Use of d- and l-glutamyl α-chloromethyl ketones (4-amino-6-chloro-5-oxohexanoic acid)

1979 ◽  
Vol 183 (3) ◽  
pp. 771-771
Keyword(s):  
Glutamate Dehydrogenase ◽  
Bovine Liver ◽  
Enzymic Activity ◽  
Asymmetric Distribution

scholarly journals The asymmetric distribution of enzymic activity between the six subunits of bovine liver glutamate dehydrogenase. Use of d- and l-glutamyl α-chloromethyl ketones (4-amino-6-chloro-5-oxohexanoic acid)

1976 ◽  
Vol 157 (3) ◽  
pp. 675-686 ◽  
Author(s):  
C G Rasool ◽  
S Nicolaidis ◽  
M Akhtar
Keyword(s):  
Glutamate Dehydrogenase ◽  
Bovine Liver ◽  
Total Activity ◽  
Enzymic Activity ◽  
Asymmetric Distribution ◽  
Chloromethyl Ketone ◽  
Inactivation Process ◽  
Time Courses ◽  
Cumulative Evidence ◽  
Related Compounds

A method for the preparation of D- and L-glutamyl alpha-chloromethyl ketones (4-amino-6-chloro-5-oxohexanoic acid) is described. These chloromethyl ketones irreversibly inactivated bovine glutamate dehydrogenase, whereas several other related compounds had no adverse effect on the activity of the enzyme. The inactivation process was shown to be due to the modification of lysine-126. The time-courses for the inactivation and the incorporation of radioactivity from tritiated L-glutamyl alpha-chloromethyl ketone into the glutamate dehydrogenase were biphasic. The results were interpreted to suggest the involvement of ‘negative co-operative’ interactions in the reactivity of lysine-126. From the cumulative evidence it is argued that the first subunit of the enzyme, which takes part in catalysis, makes the largest, and the last the smallest, contribution to the overall catalysis. It is emphasized that three of the six subunits of the enzyme may possess as much as 80% of the total activity of bovine glutamate dehydrogenase.

scholarly journals Lysine and tyrosine in the NADH inhibitory site of bovine liver glutamate dehydrogenase.

1981 ◽  
Vol 256 (22) ◽  
pp. 11866-11872
Author(s):  
K.V. Saradambal ◽  
R.A. Bednar ◽  
R.F. Colman
Keyword(s):  
Glutamate Dehydrogenase ◽  
Bovine Liver ◽  
Inhibitory Site

scholarly journals Sequence of Bovine Liver Glutamate Dehydrogenase

1971 ◽  
Vol 246 (8) ◽  
pp. 2374-2399 ◽  
Author(s):  
Michael Landon ◽  
Dennis Piszkiewicz ◽  
Emil L. Smith
Keyword(s):  
Glutamate Dehydrogenase ◽  
Bovine Liver

scholarly journals Sequence of Bovine Liver Glutamate Dehydrogenase

1973 ◽  
Vol 248 (9) ◽  
pp. 3067-3081 ◽  
Author(s):  
Dennis Piszkiewicz ◽  
Michael Landon ◽  
Emil L. Smith
Keyword(s):  
Glutamate Dehydrogenase ◽  
Bovine Liver

scholarly journals Sequence of Bovine Liver Glutamate Dehydrogenase

1971 ◽  
Vol 246 (8) ◽  
pp. 2400-2418 ◽  
Author(s):  
William J. Brattin ◽  
Emil L. Smith
Keyword(s):  
Glutamate Dehydrogenase ◽  
Bovine Liver
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