scholarly journals Conversion between renin and high-molecular-weight renin in the dog

1978 ◽  
Vol 176 (3) ◽  
pp. 977-981 ◽  
Author(s):  
S Funakawa ◽  
Y Funae ◽  
K Yamamoto
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Soluble Fraction ◽  
The Other ◽  
Kidney Cortex ◽  
Thiol Groups ◽  
Cortical Tissue ◽  
Neutral Ph ◽  
Dog Kidney ◽  
Renal Cortical Tissue

Two forms of renin, one of mol.wt. 43,000 and the other 60,000, were found in the dog kidney. Conversion between the two forms of renin was reversible at neutral pH. Though the molecular weight of renin in kidney-cortex homogenate was 43,000, it was completely converted into high-molecular-weight renin in the presence of substances that react with thiol groups. On the contrary, stored renin in the granules was the form of normal size (mol. wt. 43,000) regardless of the absence or presence of such substances. The present experiments indicated that renin is stored in the granules as the form of normal size and might be converted into high-molecular-weight renin when it is released from the granules and attached to some substance in the soluble fraction of renal-cortical tissue.

Sulphydryl Oxidation in the Mechanism of Molecular-Weight Conversion of Renin in Dog Kidney

1982 ◽  
Vol 62 (2) ◽  
pp. 157-162 ◽  
Author(s):  
Fumihiko Ikemoto ◽  
Kazuo Takaori ◽  
Hiroshi Iwao ◽  
Kenjiro Yamamoto
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Renal Cortex ◽  
Soluble Fraction ◽  
Low Molecular Weight ◽  
Oxidizing Agent ◽  
Nitrobenzoic Acid ◽  
Binding Substance ◽  
Sh Group ◽  
Dog Kidney

1. A high-molecular-weight renin (Mr 60 000) was formed by the reaction of a low-molecular-weight renin (Mr 40 000) with a renin-binding substance in canine renal cortical extract in the presence of the sulphydryl (SH) group oxidizing agent potassium tetrathionate; thus the reaction required SH oxidation. 2. Renin extracted from isolated renin granules was adsorbed on to thiopropyl Sepharose 6B, and then liberated with dithiothreitol (50 mmol/l), indicating that it possessed on SH moiety(s). 3. However, the renin was capable of reaction with the renin-binding substance even after its SH moiety (or moieties) was protected with 5,5′-dithiobis-(2-nitrobenzoic acid). 4. The high-molecular-weight renin was converted into the low-molecular-weight renin by incubation (37°C, 15 min) with cytosol (soluble fraction) of renal cortex and liver. Such converting ability was diminished after the cytosol was treated with perchloric acid or potassium tetrathionate. 5. These results suggest that the reaction of renin with the renin-binding substance does not require disulphide bond(s) and that an enzymelike substance which is sensitive to SH oxidation is involved in the conversion from the high-molecular-weight renin into the low-molecular weight renin.

Interconversion between High- and Low-Molecular-Weight Forms of Renin in Dog Kidney

1980 ◽  
Vol 59 (s6) ◽  
pp. 25s-27s ◽  
Author(s):  
K. Yamamoto ◽  
F. Ikemoto ◽  
M. Kawamura ◽  
K. Takaori
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Apparent Molecular Weight ◽  
Kidney Cortex ◽  
Low Molecular Weight ◽  
Cytosol Fraction ◽  
High Molecular Weight Form ◽  
Binding Substance ◽  
Dog Kidney ◽  
Molecular Weight Form

1. The low-molecular-weight (40 000) form of renin was converted into the high-molecular-weight (60 000) form of renin with sulphydryl oxidation, and the high-molecular-weight form of renin was re-converted into the low-molecular-weight form with a reduction of disulphide bonds in the renal cortical homogenate of the dog. Therefore, the low- and high-molecular-weight forms of renin were interconvertible. 2. The formation of high-molecular-weight form of renin required a renin binding substance which was found to be included in the cytosol fraction of kidney cortex of the dog. 3. The renin binding substance of the dog was unstable to heat and low pH, but vitally resistant to Triton X-100 and chloroform. It did not bind to concanavalin A Sepharose 4B. 4. The renin binding substance was eluted in the molecular-weight region between 156 000 and 60 000 on Sephadex G-200, and such apparent molecular weight was not altered by urea at 4 mol/l; thus molecular weight greater than the theoretically expected value of 20 000 was indicated.

Rapid Isolation of High Molecular Weight Urokinase from Native Human Urine

1982 ◽  
Vol 47 (03) ◽  
pp. 197-202 ◽  
Author(s):  
Kurt Huber ◽  
Johannes Kirchheimer ◽  
Bernd R Binder
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Human Urine ◽  
Gel Filtration ◽  
Chain Structure ◽  
The Other ◽  
Single Chain ◽  
Apparent Homogeneity ◽  
Sequential Adsorption

SummaryUrokinase (UK) could be purified to apparent homogeneity starting from crude urine by sequential adsorption and elution of the enzyme to gelatine-Sepharose and agmatine-Sepharose followed by gel filtration on Sephadex G-150. The purified product exhibited characteristics of the high molecular weight urokinase (HMW-UK) but did contain two distinct entities, one of which exhibited a two chain structure as reported for the HMW-UK while the other one exhibited an apparent single chain structure. The purification described is rapid and simple and results in an enzyme with probably no major alterations. Yields are high enough to obtain purified enzymes for characterization of UK from individual donors.

Barricyclin D1—a dimeric ellagitannin with a macrocyclic structure—and accompanying tannins from Barringtonia racemosa

2021 ◽  
Author(s):  
Shinji Yoshikawa ◽  
Lih-Geeng Chen ◽  
Morio Yoshimura ◽  
Yoshiaki Amakura ◽  
Tsutomu Hatano ◽  
...  
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Natural Resource ◽  
The Other ◽  
Hydrolysable Tannin ◽  
The Family ◽  
Macrocyclic Structure

Abstract Our examination of high molecular weight polyphenolic constituents in the leaves of Barringtonia racemosa of the family Lecythidaceae uncovered five previously undescribed ellagitannins. One, barringtin M1 (1), among them was a hydrolysable tannin monomer, while remaining four, barringtins D1 (2), D2 (3), D3 (4) and barricyclin D1 (5), were all dimers. Barricyclin D1 had a first macrocyclic structure formed from casuarictin (6) and tellimagrandin I (7), and the other ellagitannins had structures related to 5. Two additional known phenolics, valoneic acid dilactone (8) and schimawalin A (9), were also isolated from the leaves. These results suggested that the leaves of B. racemosa is a natural resource rich in hydrolysable tannin oligomers.

Fractionation of Rubber

1941 ◽  
Vol 14 (1) ◽  
pp. 1-14 ◽  
Author(s):  
George F. Bloomfield ◽  
Ernest Harold Farmer
Keyword(s):  
Molecular Weight ◽  
Soluble Fraction ◽  
Insoluble Fraction ◽  
The Other ◽  
Major Fraction ◽  
Low Concentrations ◽  
Fractional Dissolution ◽  
Judicious Choice ◽  
Hydrocarbon Molecules ◽  
Soluble Fractions

Abstract Latex rubber which has been purified to the point at which it contains an insignificant amount of nitrogen can be separated by fractional dissolution in a mixture of petroleum and acetone into a series of hydrocarbon fractions of decreasing solubility and increasing molecular magnitude. All these fractions except the highest are soluble in petroleum and in benzene. Crepe rubber, on the other hand, appears invariably to contain a small, most-soluble fraction of oxygenated rubber, and a small similar quite insoluble fraction of material of high molecular weight. Between these extremes the rubber can be divided into fractions of increasing molecular weight, although, up to the present, about 70 per cent of the total rubber has appeared in a single fraction. It may be possible later, by judicious choice of another pair of solvents, to resolve this major fraction into a series of subfractions. Kemp and Peters refer to the effect of polar nonsolvents in reducing the viscosity of rubber solutions and also in assisting to bring gel rubber into solution, phenomena to which the polar molecules conceivably contribute by countering the forces of association between the rubber molecules. The present series of fractionations was conducted throughout in the presence of a polar nonsolvent (acetone), and hence may be considered to approach towards a separation of true rubber molecules as distinct from molecular aggregates. It is found, however, that, whereas the more soluble fractions of acetone-extracted crepe rubber contain small proportions of nitrogen, the least soluble fractions contain substantial proportions. Any effect which the nitrogenous material may have in assisting to link together hydrocarbon molecules to which it is attached, i. e., in contributing to the high-molecular condition of a portion of natural rubber, remains at present uncertain in character. The fractions of rubber, and especially the higher ones, show a strong tendency to become insoluble when they have once been freed from the last traces of solvent. It seems doubtful whether the decreased solubility is due to oxygen as it would require to be effective at exceedingly low concentrations.

scholarly journals ELECTROPHORETIC STUDIES ON PR8 INFLUENZA VIRUS

1944 ◽  
Vol 80 (6) ◽  
pp. 549-559 ◽  
Author(s):  
Gail Lorenz Miller ◽  
Max A. Lauffer ◽  
W. M. Stanley
Keyword(s):  
Molecular Weight ◽  
Influenza Virus ◽  
High Molecular Weight ◽  
Isoelectric Point ◽  
High Speed ◽  
The Other ◽  
Active Virus ◽  
Electrophoresis Method

Crude preparations of PR8 influenza virus, obtained by high-speed centrifugation, contain two electrophoretically distinct components. One of these, present to the extent of 10 to 20 per cent, was identified by electrophoresis, centrifuge, and activity tests, as an impurity similar to or identical with a high molecular weight acidic substance shown by Knight to be elaborated by normal uninfected embryos. The other component, present to the extent of 80 to 90 per cent, appeared to represent the active virus. The virus fraction was separated from the impurity by repeated fractional centrifugation. It then appeared homogeneous in the analytical centrifuge and in the Tiselius apparatus, and possessed an isoelectric point at pH 5.3 as measured by the micro-electrophoresis method.

scholarly journals A high molecular weight soluble fraction of tempeh protects against fluid losses in Escherichia coli-infected piglet small intestine

2007 ◽  
Vol 98 (2) ◽  
pp. 320-325 ◽  
Author(s):  
Jeroen L. Kiers ◽  
M. J. Rob Nout ◽  
Frans M Rombouts ◽  
Marius J. A. Nabuurs ◽  
Jan van der Meulen
Keyword(s):  
Escherichia Coli ◽  
Molecular Weight ◽  
Small Intestine ◽  
High Molecular Weight ◽  
Internal Control ◽  
Soluble Fraction ◽  
Fluid Secretion ◽  
Fluid Absorption ◽  
Fluid Losses ◽  
Etec Infection

Enterotoxigenic Escherichia coli (ETEC) is an important cause of diarrhoea in children and piglets. Infection of ETEC results in fluid secretion and electrolyte losses in the small intestine. In this study the effects of tempeh, a traditional fungal fermented soyabean product, on fluid losses induced by ETEC infection in piglets was investigated. Pairs of ETEC-infected and non-infected small intestinal segments of piglets were perfused simultaneously for 8 h with pre-digested tempeh, its supernatant and saline as an internal control. In saline perfused segments, ETEC infection reduced net fluid absorption by more than 500 μl/cm2, whereas this reduction was significantly less for pre-digested tempeh and its supernatant (75 and 282 μl/cm2, respectively). The supernatant of pre-digested tempeh was also compared with its permeate and retentate fractions. These fractions were created by ultra-filtration and contained respectively low and high molecular weight (>5 kDa) compounds. Again ETEC infection caused a significant reduction of net fluid absorption when perfused with saline (386 μl/cm2) and also with the permeate fraction (300 μl/cm2), but much less with the supernatant and the retentate fraction (125 and 140 μl/cm2, respectively). The reduction in net fluid absorption upon ETEC infection when perfused with supernatant of either undigested or pre-digested tempeh was not different. Therefore from this study it can be concluded that a high molecular weight soluble fraction of tempeh is able to protect against fluid losses induced by ETEC, suggesting that this could play a potential role in controlling ETEC-induced diarrhoea.

Mechanism of Rupture of High Polymers

1961 ◽  
Vol 34 (1) ◽  
pp. 101-118 ◽  
Author(s):  
V. E. Gul
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Wide Distribution ◽  
The Other ◽  
Polymer Materials ◽  
High Polymer ◽  
Structural Elements ◽  
Synthetic Fibers ◽  
High Molecular Weight Material ◽  
Grinding Conditions

Abstract In connection with the wide distribution of high molecular weight compounds and the great successes in the field of synthesis of these materials, the study of the general principles of the processes of irreversible deformation and rupture of high polymer materials may be included among the important tasks of science and technology. In some cases it is desired to increase to the maximum the strength of the high molecular weight material, as for example in preparing articles from synthetic fibers, rubbers, plastics, etc., while on the other hand in others an increase in strength is undesirable. Thus, for example, food products, into whose composition high molecular weight materials are introduced, should naturally not be too strong. The preparation of reclaim from used rubber articles includes grinding. Here it is attempted to find such a combination of grinding conditions that will proceed with the minimum consumption of energy. In order to have the possibility of increasing or decreasing the strength of the high molecular weight substances, it is necessary to clarify the mechanism of ruptures and to know what is the relation between the forces of reaction between the structural elements of the material and tensile strength.

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