scholarly journals The metabolism of high-molecular-weight ribonucleic acid in hypothalamic and cortical regions of the developing female rat brain

1978 ◽  
Vol 176 (2) ◽  
pp. 511-521 ◽  
Author(s):  
C Hall ◽  
L Lim
Keyword(s):  
Molecular Weight ◽  
Rat Brain ◽  
High Molecular Weight ◽  
Post Partum ◽  
Specific Radioactivity ◽  
Nucleocytoplasmic Transport ◽  
Brain Regions ◽  
Female Rat ◽  
Rna Transport ◽  
Cytoplasmic Transport

The regional metabolism of high-molecular-weight RNA in the developing female rat brain was investigated after the intracranial injection of [32P]P1. The synthesis of polyadenylated RNA relative to high-molecular-weight RNA was determined after oligo(dT)-cellulose chromatography of total cellular high-molecular-weight RNA labelled after 4h. In both hypothalamus and cortex this synthesis was significantly higher during the first 10 days post partum than at subsequent ages. In both regions apparently more mRNA is synthesized in the young. The ratio of the specific radioactivity of cytoplasmic high-molecular-weight RNA relative to that of the nucleus, measured after a 48 h period of labelling, was considered to be an index of the nucleocytoplasmic transport of newly synthesized RNA [Berthold & Lim (1976) Biochem. J. 154, 529–539]. In the cortex, nucleo-cytoplasmic RNA transport in rats aged up to 20 days was significantly higher than in older rats, with the maximal value being attained between 16 and 19 days post partum. In contrast, in the hypothalamus, nucleo-cytoplasmic transport of RNA was low during the neonatal period and comparable with that of the mature animal. However, there were two periods of increased transport at later stages of development, the first between 15 and 19 days post partum and the second between 25 and 29 days post partum. These prepubertal changes in the nucleo-cytoplasmic transport of RNA in the female hypothalamus during weeks 3 and 4 post partum are coincident with other reported changes occurring during sexual differentiation. Differences in the timing of the maturational changes of the two brain regions thus appear to be reflected in developmental changes in RNA transport.

Movement of [U-14C]glucose Carbon into and Subsequent Release from lipids and High-Molecular-Weight Constituents of Rat Brain, Liver, and Heart In Vivo

1972 ◽  
Vol 50 (1) ◽  
pp. 91-105 ◽  
Author(s):  
R. Vrba ◽  
Anna Winter
Keyword(s):  
Amino Acids ◽  
Molecular Weight ◽  
Rat Brain ◽  
High Molecular Weight ◽  
High Speed ◽  
Time Course ◽  
Specific Radioactivity ◽  
Brain Proteins ◽  
Glucose Carbon

After subcutaneous injection of [U-14C]glucose into rats the amount of 14C incorporated in vivo into proteins was always higher than into lipids in brain, liver, and heart. The specific radioactivity of brain proteins was higher than those of liver and heart. Blood-brain comparisons show that protein carbon is derived continuously from glucose in the brain in situ and not as a result of deposition of amino acids or proteins from the circulation. Seventy-two percent of 14C in purified brain protein fractions was found in the amino acids of the hydrolysates of these fractions, mainly in alanine, glutamic, and aspartic acids. Maximum labelling was reached about 4 h after injection of [U-14C]glucose. Elimination of 14C from three classes of brain proteins (high-speed supernatant, particulate deoxycholate extractable, and residual) followed a biphasic time-course. The extent of labelling of, and the rate of elimination of 14C from, the three classes of rat brain proteins were very similar. The fate of 14C in the other investigated tissue fractions of brain, liver, and heart was compared with the fate of 14C in brain proteins.The results lend further support to the previously published suggestion that: (a) brain does not contain appreciable amounts of metabolically inert proteins or of proteins with turnover rates significantly higher than the mean for the bulk of brain proteins; (b) glucose carbon participates at a different rate and to a different extent in the metabolism of high-molecular-weight constituents of brain as compared to liver, heart, and plasma proteins; (c) the continuous conversion of glucose carbon into protein is an important part of the maintenance of the homeostasis of tissue proteins in vivo.

Maternal Exposure to Δ 9 -Tetrahydrocannabinol ( Δ 9 -THC) Alters Indolamine Levels and Turnover in Adult Male and Female Rat Brain Regions

1997 ◽  
Vol 43 (2) ◽  
pp. 173-178 ◽  
Author(s):  
Francisco Molina-Holgado ◽  
Francisco J. Alvarez ◽  
Isabel Gonzalez ◽  
Maria T. Antonio ◽  
Maria L. Leret
Keyword(s):  
Rat Brain ◽  
Adult Male ◽  
Brain Regions ◽  
Maternal Exposure ◽  
Female Rat ◽  
Male And Female ◽  
Rat Brain Regions

scholarly journals Molecular cloning and expression of novel sulphotransferase-like cDNAs from human and rat brain

2000 ◽  
Vol 346 (3) ◽  
pp. 857-864 ◽  
Author(s):  
Charles N. FALANY ◽  
Xiaowei XIE ◽  
Jin WANG ◽  
Jorge FERRER ◽  
Josie L. FALANY
Keyword(s):  
Rat Brain ◽  
Polyclonal Antibodies ◽  
Brain Regions ◽  
Female Rats ◽  
Cloning And Expression ◽  
Female Rat ◽  
Affinity Tag ◽  
Calculated Molecular Mass ◽  
Male And Female ◽  
Male And Female Rats

The sulphotransferase (SULT) gene family is involved with the conjugation of many small drugs, xenobiotics and endogenous compounds. In this report, we describe the cloning and expression of novel cDNAs from human and rat brain, which are structurally related to the SULTs. These cDNAs have been termed ‘brain sulphotransferase-like’ (BR-STL), because of their similarity to the SULTs and their selective expression in brain tissue. The proteins encoded by the human and rat BR-STL cDNAs (hBR-STL-1 and rBR-STL cDNA respectively), denoted as hBR-STL and rBR-STL, are 98% identical and 99% similar in sequence. The hBR-STL-1 cDNA contains an 852-nt open reading frame encoding a 284-amino-acid protein with a calculated molecular mass of 33083 Da. Northern-blot analyses of RNA isolated from eight human tissues indicate that the hBR-STL message is selectively expressed in brain. Characterization of different brain regions showed that message levels were highest in cortical brain regions. rBR-STL message levels were relatively low in brains of 1-day-old male and female rats, but increased to adult levels in RNA from 7-day-old rats, and remained at that level in adult animals. The hBR-STL and rBR-STL cDNAs were expressed in both Escherichia coli and Sf9 insect cells in the presence or absence of an N-terminal histidine-affinity tag (His-tag). Polyclonal antibodies were raised in chickens against purified His-tagged hBR-STL, and were used to detect the presence of rBR-STL in adult male and female rat brain cytosol. The high degree of sequence conservation, and the selective localization of the BR-STL message in brain, suggest an important function in the central nervous system.

scholarly journals The metabolism of high-molecular-weight ribonucleic acid including polyadenylated species, in the developing rat brain

1976 ◽  
Vol 154 (2) ◽  
pp. 517-527 ◽  
Author(s):  
W Berthold ◽  
L Lim
Keyword(s):  
Molecular Weight ◽  
Brain Stem ◽  
Rat Brain ◽  
High Molecular Weight ◽  
Newborn Rats ◽  
Total Rna ◽  
Adult Rat ◽  
Polyadenylated Rna ◽  
Old Rats ◽  
Developing Rat

High-molecular-weight RNA was isolated from rat brain at various times after the intracranial administration of [32P]Pi. The synthesis of 28S and 18S rRNA could be detected within 1h of the injection of the radioactive precursor and appeared to be more pronounced, relative to other high-molecular-weight RNA, in the brains of older rats compared with those of newborn rats. Polyadenylated RNA, representing most mRNA and their precursors, was isolated by chromatography on oligo(dT)-cellulose. The contribution of this polyadenylated RNA to total RNA synthesis was investigated in the cerebral cortex and the phylogenetically older brain stem at different stages in the development of the rats by using a 5h period of labelling as an arbitrary index of transcription. In the brain stem the proportion of labelled polyadenylated RNA comprised 27-30% of the total RNA. The corresponding values for the cortex decreased from 34% in newborn rats to 23% in 40-150-day-old rats. These data indicated that proportionately more polyadenylated RNA is synthesized in the cortex of the newborn than in the adult rat and that there is a progressive decrease in the synthesis of polyadenylated RNA relative to rRNA during development.

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