scholarly journals Metabolism and the triggering of germination of Bacillus megaterium. Concentrations of amino acids, organic acids, adenine nucleotides and nicotinamide nucleotides during germination

1978 ◽  
Vol 174 (2) ◽  
pp. 627-634 ◽  
Author(s):  
I R Scott ◽  
D J Ellar
Keyword(s):  
Amino Acids ◽  
Organic Acids ◽  
Bacillus Megaterium ◽  
Spore Germination ◽  
Free Amino Acids ◽  
Free Amino ◽  
Adenine Nucleotides ◽  
Analytical Techniques ◽  
Biochemical Changes ◽  
Bacterial Spore

A considerable amount of evidence suggests that metabolism of germinants or metabolism stimulated by them is involved in triggering bacterial-spore germination. On the assumption that such a metabolic trigger might lead to relatively small biochemical changes in the first few minutes of germination, sensitive analytical techniques were used to detect any changes in spore components during the L-alanine-triggered germination of Bacillus megaterium KM spores. These experiments showed that no changes in spore free amino acids or ATP occurred until 2-3 min after L-alanine addition. Spores contained almost no oxo acids (pyruvate, alpha-oxoglutarate, oxaloacetate), malate or reduced NAD. These compounds were again not detectable until 2-3 min after addition of germinants. It is suggested, therefore, that metabolism associated with these intermediates is not involved in the triggering of germination of this organism.

scholarly journals Release of Small Molecules during Germination of Spores of Bacillus Species

2008 ◽  
Vol 190 (13) ◽  
pp. 4759-4763 ◽  
Author(s):  
Barbara Setlow ◽  
Paul G. Wahome ◽  
Peter Setlow
Keyword(s):  
Amino Acids ◽  
Small Molecules ◽  
Spore Germination ◽  
Free Amino Acids ◽  
Free Amino ◽  
Dipicolinic Acid ◽  
Adenine Nucleotides ◽  
Bacillus Species ◽  
Hydrolysis Of ◽  
Selective Change

ABSTRACT Free amino acids, dipicolinic acid, and unidentified small molecules were released early in Bacillus spore germination before hydrolysis of the peptidoglycan cortex, but adenine nucleotides and 3-phosphoglycerate were not. These results indicate that early in germination there is a major selective change in the permeability of the spore's inner membrane.

Effects of Excess Methionine Ingestion on Hepatic Phosphate, Adenine Nucleotides and Free Amino Acids in the Rat

1982 ◽  
Vol 112 (5) ◽  
pp. 833-840 ◽  
Author(s):  
Daniel Fau ◽  
Marc Chanez ◽  
Brigitte Bois-Joyeux ◽  
Brigitte Delhomme ◽  
Jean Peret
Keyword(s):  
Amino Acids ◽  
Free Amino Acids ◽  
Free Amino ◽  
Adenine Nucleotides

scholarly journals Quantification of Phytochemicals from CommercialSpirulinaProducts and Their Antioxidant Activities

2016 ◽  
Vol 2016 ◽  
pp. 1-13 ◽  
Author(s):  
Naif Abdullah Al-Dhabi ◽  
Mariadhas Valan Arasu
Keyword(s):  
Amino Acids ◽  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Free Amino Acids ◽  
Free Amino ◽  
High Performance ◽  
Antioxidant Activities ◽  
Analytical Techniques ◽  
Essential Amino Acids

The present study aimed to profile the polyunsaturated fatty acids, sugars, free amino acids, and polyphenols in 37 varieties ofSpirulinacommonly available in the market using gas chromatography and high performance liquid chromatography. In addition, the biological potentials of theSpirulinasamples were evaluated by analysing thein vitroantioxidant activities using various analytical techniques. The analyses revealed the presence of 13 polyunsaturated fatty acids, 18 amino acids, 7 sugars, and polyphenols. The polyunsaturated fatty acids contents were varied betweenSpirulinasamples. The total polyunsaturated fatty acids amount was 4.25 mg/100 g, and the average among of sapienic acid detected was 2.25 mg/100 g, which was followed by linoleic acid (16.7%) andγ-linolenic acid (14%). Among the 7 sugars, the hexose levels were the highest (73.85%). The total amino acids contents ranged from 11.49 to 56.14 mg/100 g, and the individual essential amino acids accounted for 17% to 39.18%. The “natural” tablets exhibited the highest polyphenols levels (24 mg/g). All of theSpirulinasamples expressed dose-dependent antioxidant activities. The polyunsaturated fatty acids, sugars, free amino acids, and polyphenols contents varied widely, and the variations in these compounds between theSpirulinasamples were significant.

Metabolism of proline-U-14C during germination of barley

1968 ◽  
Vol 46 (10) ◽  
pp. 1331-1334 ◽  
Author(s):  
P. V. Sane ◽  
Saul Zalik
Keyword(s):  
Amino Acids ◽  
Organic Acids ◽  
Free Amino Acids ◽  
Free Amino ◽  
Etiolated Seedlings ◽  
Embryo Metabolism

Proline-U-14C injected into the endosperm of 3-day-old etiolated seedlings of Montcalm barley and its mutant was readily translocated to the embryo. Of label present in the embryo, over 65% was accounted for by protein and free amino acids and about 25% was present in organic acids. Proline accounted for over 90% of the label in free amino acids of the endosperm but accounted for less than 50% of label in this fraction in the embryo. Proline was readily converted to glutamate in the embryo. Metabolism of proline was similar in both lines.

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