scholarly journals Incorporation of ribonucleic acid in vitro into dense ribonucleoprotein-like materials by isolated rat liver nuclei

1978 ◽  
Vol 174 (2) ◽  
pp. 503-508 ◽  
Author(s):  
I Suzuka
Keyword(s):  
Rat Liver ◽  
Ribonucleic Acid ◽  
Free System ◽  
Isolated Rat Liver ◽  
Cell Free System ◽  
Rat Liver Nuclei ◽  
A Cell ◽  
Liver Nuclei ◽  
Isolated Rat

A cell-free system of isolated rat liver nuclei is described which permits an active incorporation of newly synthesized RNA into ‘dense’ ribonucleoprotein-like materials. The reaction is stimulated with increasing amounts of cytosol protein isolated from rat liver. This indicates that cytosol protein plays an important role in the formation of such material.

scholarly journals Ribonucleic acid synthesis in isolated rat liver nuclei under conditions of ribonucleic acid processing and transport

1975 ◽  
Vol 147 (2) ◽  
pp. 193-197 ◽  
Author(s):  
D J McNamara ◽  
J Racevskis ◽  
D E Schumm ◽  
T E Webb
Keyword(s):  
Rat Liver ◽  
Ribonucleic Acid ◽  
Acid Synthesis ◽  
Free System ◽  
Isolated Rat Liver ◽  
Rat Liver Nuclei ◽  
Acid Processing ◽  
A Cell ◽  
Liver Nuclei ◽  
Isolated Rat

A cell-free system is described which permits a significant and prolonged synthesis of RNA in isolated rat liver nuclei, under conditions previously demonstrated to support normal nuclear processing and transport of both rRNA and mRNA. The system contains cytosol but not (NH4)2SO4 or other non-physiological components. Evidence is presented for cytosol factors which stimulate ribosomal, and to a lesser degree, non-ribosomal RNA synthesis.

scholarly journals Inhibition by α-amanitin of ribonucleic acid polymerase solubilized from rat liver nuclei

1970 ◽  
Vol 116 (2) ◽  
pp. 177-180 ◽  
Author(s):  
F. Novello ◽  
L. Fiume ◽  
F. Stirpe
Keyword(s):  
Rna Polymerase ◽  
Ammonium Sulphate ◽  
Rat Liver ◽  
Ribonucleic Acid ◽  
Isolated Rat Liver ◽  
Rat Liver Nuclei ◽  
Liver Nuclei ◽  
Isolated Rat

1. α-Amanitin inhibits in vitro the RNA polymerase solubilized from isolated rat liver nuclei. 2. In contrast with previous observations with whole nuclei, the inhibition occurs approximately to the same extent in the presence and in the absence of ammonium sulphate. 3. Evidence is presented that the toxin acts by interacting with the enzyme itself and not with DNA or other components.

scholarly journals The effects of copper and other ions on the ribonucleic acid polymerase activity of isolated rat liver nuclei

1969 ◽  
Vol 111 (1) ◽  
pp. 115-119 ◽  
Author(s):  
F. Novello ◽  
F. Stirpe
Keyword(s):  
Rna Polymerase ◽  
Ammonium Sulphate ◽  
Rat Liver ◽  
Ribonucleic Acid ◽  
Polymerase Activity ◽  
Isolated Rat Liver ◽  
Rat Liver Nuclei ◽  
Liver Nuclei ◽  
Isolated Liver ◽  
Isolated Rat

1. The effects of various ions on the Mg2+- and Mn2+/ammonium sulphate-activated RNA polymerase activities of isolated liver nuclei were studied. 2. The Mg2+-activated RNA polymerase reaction was inhibited by more than 60% by Cd2+, SeO32−, Be2+, Cu2+, Co2+, Ca2+ and La3+, all at 1mm concentrations. 3. The Mn2+/ammonium sulphate-activated RNA polymerase reaction was strongly inhibited by Hg2+, Cd2+, Cu2+ and Ag+. The effect of Hg2+, Cd2+ and Ag+ was relieved by cysteine or mercaptoethanol. 4. Inhibition by Cu2+ was not affected by addition of DNA, and was relieved only partially by EDTA or histidine. 5. No changes of RNA polymerase activities were observed in nuclei isolated from the liver of rats treated with copper albuminate.

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