scholarly journals Modeccin, the toxin of Adenia digitata. Purification, toxicity and inhibition of protein synthesis in vitro

1978 ◽  
Vol 174 (2) ◽  
pp. 491-496 ◽  
Author(s):  
A Gasperi-Campani ◽  
L Barbieri ◽  
E Lorenzoni ◽  
L Montanaro ◽  
S Sperti ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Affinity Chromatography ◽  
Ehrlich Ascites ◽  
Ehrlich Ascites Cells ◽  
Inhibition Of Protein Synthesis ◽  
Sepharose 4B ◽  
Rabbit Reticulocytes ◽  
In Cells

1. Modeccin, the toxin of Adenia digitata (Modecca digitata), was purified from the roots of this plant by affinity chromatography on Sepharose 4B. 2. This toxin is a protein with mol.wt. 57000, which on treatment with 2-mercaptoethanol can be dissociated into two subunits of mol.wts. 25000 and 32000. 3. Modeccin inhibits protein synthesis in vitro in a lysate of rabbit reticulocytes and in Ehrlich ascites cells; the effect on cells is decreased in the presence of lactose. 4. Dissociation of modeccin into subunits decreases the toxicity to animals and the inhibition of protein synthesis in cells, but enhances the inhibition of protein synthesis in the lysate system.

scholarly journals Studies on the proteins from the seeds of Croton tiglium and of Jatropha curcas. Toxic properties and inhibition of protein synthesis in vitro

1976 ◽  
Vol 156 (1) ◽  
pp. 1-6 ◽  
Author(s):  
F Stirpe ◽  
A Pession-Brizzi ◽  
E Lorenzoni ◽  
P Strocchi ◽  
L Montanaro ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Jatropha Curcas ◽  
Crude Protein ◽  
Croton Tiglium ◽  
Ehrlich Ascites ◽  
Maximum Inhibition ◽  
Ehrlich Ascites Cells ◽  
Inhibition Of Protein Synthesis ◽  
Reticulocyte Lysate

1. Proteins extracted from the seeds of the Euphorbiaceae croton tiglium and Jatropha curcas were separated into three major peaks (I, II, and III) by Sephadex chromatography. 2. The crude protein from both seeds and peaks I and II from Croton and peak I from Jatropha were toxic to mice, to different extents. 3. The crude protein and peak I and peak II from both seeds, inhibited protein synthesis by a reticulocyte lysate; maximum inhibition was exerted by peak II from both seeds. None of these preparations affected protein synthesis in vitro by Ehrlich ascites cells.

scholarly journals The Relation between Protein Synthesis and Lipide Accumulation in L Strain Cells and Ehrlich Ascites Cells

1959 ◽  
Vol 5 (3) ◽  
pp. 421-431 ◽  
Author(s):  
Donald W. King ◽  
Edward L. Socolow ◽  
Klaus G. Bensch
Keyword(s):  
Tissue Culture ◽  
Protein Synthesis ◽  
Cell Division ◽  
Growth Period ◽  
Cholesterol Content ◽  
Cell Ratio ◽  
L Cells ◽  
Ehrlich Ascites ◽  
Ehrlich Ascites Cells

It has long been known that fat accumulates in old injured cells both in tissue culture and in many mammalian disease states. The use of L cells grown in suspension tissue culture permitted the opportunity to study conditions in which lipide accumulation could be retarded or accelerated. These cultures exhibit a three-phase growth curve which is similar to that previously found with bacteria and consists of a lag period, logarithmic growth period, and stationary period. Daily aliquots were removed from cultures going through these phases and protein and cholesterol content correlated with cell division. It was found that L cells gradually accumulated lipide in the cell concurrent with retardation of cell division and protein synthesis. Conversely old lipide-laden cells, placed in fresh media and encouraged to active division with net protein synthesis progressed from a high to a low lipide/cell ratio over a period of 2 to 4 days. An amino acid analogue p-fluorophenylalanine and a mitotic inhibitor, colchicine, also markedly increased the lipide/cell ratio. Similar results were found in in vitro experiments with Ehrlich ascites cells.

scholarly journals Inhibition of protein synthesis in vitro by proteins from the seeds of Momordica charantia (bitter pear melon)

1980 ◽  
Vol 186 (2) ◽  
pp. 443-452 ◽  
Author(s):  
L Barbieri ◽  
M Zamboni ◽  
E Lorenzoni ◽  
L Montanaro ◽  
S Sperti ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Affinity Chromatography ◽  
Potent Inhibitor ◽  
Artemia Salina ◽  
Momordica Charantia ◽  
Molar Excess ◽  
Reticulocyte Lysate ◽  
Inhibitor Of Protein Synthesis ◽  
Sepharose 4B

1. A haemagglutinating lectin was purified from the seeds of Momordica charantia by affinity chromatography on Sepharose 4B and on acid-treated Sepharose 6B. It has mol.wt. 115 000 and consists of four subunits, of mol.wts. 30 500, 29 000, 28 500 and 27 000. 2. The lectin inhibits protein synthesis by a rabbit reticulocyte lysate with an ID50 (concentration giving 50% inhibition) of approx. 5 micrograms/ml. Protein synthesis by Yoshida ascites cells is partially inhibited by the lectin at a concentration of 100 micrograms/ml. 3. From the same seeds another protein was purified which has mol.wt. 23 000 and is a very potent inhibitor of protein synthesis in the lysate system, with an ID50 of 1.8 ng/ml. This inhibitor has no effect on protein synthesis by Yoshida cells, and has no haemagglutinating properties. 4. Artemia salina ribosomes preincubated with the lectin or with the inhibitor lose their capacity to perform protein synthesis. The proteins seem to act catalytically, since they inactivate a molar excess of ribosomes. 5. The lectin and the inhibitor are somewhat toxic to mice, the LD50 being 316 and 340 micrograms/100 g body wt. respectively.

EFFECT OF X-IRRADIATION ON DNA SYNTHESIS IN EHRLICH ASCITES CARCINOMA CELLS

1965 ◽  
Vol 43 (7) ◽  
pp. 859-864 ◽  
Author(s):  
Shan-Ching Sung
Keyword(s):  
Protein Synthesis ◽  
Dna Synthesis ◽  
Rna Synthesis ◽  
Ehrlich Ascites Carcinoma ◽  
Carcinoma Cells ◽  
Total Rna ◽  
Ehrlich Ascites ◽  
Ehrlich Ascites Cells ◽  
X Irradiation

The rate of DNA synthesis in Ehrlich ascites cells measured immediately after X-irradiation of 500 r for 6 minutes in vitro showed about 15% reduction. However, if X-irradiation was followed by preincubation of the cells, the subsequent synthesis of DNA in the X-irradiated cells was markedly inhibited. Under the same condition, the uptake of thymidine-2-C14, uridine-2-C14, adenine-8-C14, and glycine-1-C14, and protein synthesis in the X-irradiated cells were found to be almost the same as those in the non-irradiated control. RNA synthesis measured as total RNA was only slightly inhibited.

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