scholarly journals Effect of the protein-synthesis-initiation inhibitor 2-(4-methyl-2,6-dinitroanilino)-N-methylpropionamide on ribonucleic acid synthesis in radish seedlings

1978 ◽  
Vol 171 (3) ◽  
pp. 607-611 ◽  
Author(s):  
L Aspart ◽  
R Cooke ◽  
M Delseny ◽  
Y Guitton
Keyword(s):  
Protein Synthesis ◽  
Ribonucleic Acid ◽  
Acid Synthesis ◽  
Ribosomal Genes ◽  
Rrna Synthesis ◽  
Polyadenylated Rna ◽  
Protein Synthesis Initiation ◽  
Radish Seedlings

2-(4-Methyl-2,6-dinitroanilino)-N-methylpropionamide, an inhibitor of initiation of protein synthesis in plants, selectively alters rRNA synthesis. Ribosomal genes are transcribed in the absence of protein synthesis, but processing of the transcripts is impaired. This results in an accumulation of the 2.3 × 10(6)-dalton pre-rRNA. Synthesis of polyadenylated RNA is not affected by the drug.

scholarly journals Dependence of nucleus-directed rRNA synthesis upon mitochondrial protein synthesis in Tetrahymena.

1982 ◽  
Vol 2 (5) ◽  
pp. 508-516 ◽  
Author(s):  
L Ruben ◽  
A B Hooper
Keyword(s):  
Protein Synthesis ◽  
Rna Synthesis ◽  
Mitochondrial Protein ◽  
Tetrahymena Pyriformis ◽  
Cellular Protein ◽  
Rrna Synthesis ◽  
Mitochondrial Protein Synthesis ◽  
Mitochondrial Rrna ◽  
Polyadenylated Rna ◽  
Nuclear Rna

The antibiotic chloramphenicol selectively inhibited mitochondrial protein synthesis in the ciliate protozoan Tetrahymena pyriformis GL. Secondary to the inhibition of mitochondrial protein synthesis was an inhibition of nuclear RNA synthesis at a time before inhibition of cellular protein and DNA synthesis. Of the stable non-polyadenylated RNA species in Tetrahymena, the addition of chloramphenicol resulted specifically in the inhibition of synthesis of 28S + 17S and 5S rRNA transcripts. By contrast, syntheses of 4S tRNA and 21S mitochondrial rRNA were not as extensively inhibited. The addition of 60 microM hemin before the addition of chloramphenicol partially protected against the inhibition of RNA synthesis. These data indicate that continued synthesis of nucleus-directed rRNA is linked to the synthesis of mitochondrial proteins in Tetrahymena.

scholarly journals Early ribonucleic acid synthesis during the germination of rye (Secale cereale) embryos and the relationship to early protein synthesis

1975 ◽  
Vol 148 (3) ◽  
pp. 381-387 ◽  
Author(s):  
S Sen ◽  
P I Payne ◽  
D J Osborne
Keyword(s):  
Protein Synthesis ◽  
Nucleic Acid ◽  
Secale Cereale ◽  
Ribonucleic Acid ◽  
Acid Synthesis ◽  
Fingerprint Analysis ◽  
5S Rna ◽  
Early Protein ◽  
Rrna Molecule ◽  
The Relationship

Incorporation studies with radioactive precursors showed that synthesis of protein and RNA is initiated in germinating embryos of rye within the first hour of imbibition of water. By polyacrylamide-gel fractionations of radioactive nucleic acid components, the appearance of products of transcription of the genome was shown to follow the sequence: heterogeneous (ribonuclease-sensitive) RNA, 4S and 5S RNA by 20min, 31S and 25S rRNA by 40min, and 18S RNA by 60min. “Fingerprint’ analysis of T1-ribonuclease digests show that all the large oligonucleotides present in 25S and 18S RNA are present in the 31S species, indicating that 31S RNA is the precursor rRNA molecule to both 25S and 18S RNA. The importance of these early RNA syntheses and in particular the possible template function of the heterogeneous RNA is discussed in relation to the concept of long-lived mRNA and the coding for protein synthesis in the first hours of germination.

Dependence of nucleus-directed rRNA synthesis upon mitochondrial protein synthesis in Tetrahymena

1982 ◽  
Vol 2 (5) ◽  
pp. 508-516
Author(s):  
L Ruben ◽  
A B Hooper
Keyword(s):  
Protein Synthesis ◽  
Rna Synthesis ◽  
Mitochondrial Protein ◽  
Tetrahymena Pyriformis ◽  
Cellular Protein ◽  
Rrna Synthesis ◽  
Mitochondrial Protein Synthesis ◽  
Mitochondrial Rrna ◽  
Polyadenylated Rna ◽  
Nuclear Rna

The antibiotic chloramphenicol selectively inhibited mitochondrial protein synthesis in the ciliate protozoan Tetrahymena pyriformis GL. Secondary to the inhibition of mitochondrial protein synthesis was an inhibition of nuclear RNA synthesis at a time before inhibition of cellular protein and DNA synthesis. Of the stable non-polyadenylated RNA species in Tetrahymena, the addition of chloramphenicol resulted specifically in the inhibition of synthesis of 28S + 17S and 5S rRNA transcripts. By contrast, syntheses of 4S tRNA and 21S mitochondrial rRNA were not as extensively inhibited. The addition of 60 microM hemin before the addition of chloramphenicol partially protected against the inhibition of RNA synthesis. These data indicate that continued synthesis of nucleus-directed rRNA is linked to the synthesis of mitochondrial proteins in Tetrahymena.

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