scholarly journals Control of glucose metabolism in isolated acini of the lactating mamary gland of the rat. Effects of oleate on glucose utilization and lipogenesis

1978 ◽  
Vol 170 (3) ◽  
pp. 609-613 ◽  
Author(s):  
A M Robinson ◽  
D H Williamson
Keyword(s):  
Mammary Gland ◽  
Glucose Metabolism ◽  
Glucose Utilization ◽  
Substrate Utilization ◽  
Inhibitory Effects ◽  
Rat Mammary Gland

Oleate (1mM) had only small inhibitory effects on glucose utilization and lipogenesis in acini isolated from rat mammary gland. Esterification of [1-14C]oleate was unaffected by insulin but were decreased by 60% by acetoacetate (2mM). Glycerol (1mM), but not insulin, relieved this inhibition. These experiments provide further support for the role of acetoacetate in regulating substrate utilization by the gland.

scholarly journals Control of glucose metabolism in isolated acini of the lactating mammary gland of the rat. The ability of glycerol to mimic some of the effects of insulin

1977 ◽  
Vol 168 (3) ◽  
pp. 465-474 ◽  
Author(s):  
A M Robinson ◽  
D H Williamson
Keyword(s):  
Mammary Gland ◽  
Glucose Metabolism ◽  
Glucose Uptake ◽  
Glucose Utilization ◽  
Homogeneous Population ◽  
Furoic Acid ◽  
Lactating Mammary Gland ◽  
Rat Mammary Gland ◽  
Pyruvate Ratio

Inhibition of glucose uptake by acetoacetate and relief of this inhibition by insulin found previously in slices of rat mammary gland [Williamson, McKeown & Ilic (1975) Biochem. J. 150. 145-152] was confirmed in acini, which represent a more homogeneous population of cells. Glycerol (1mM) behaved like insulin (50 minuits/ml) in its ability to relieve the inhibition of glucose (5 mM) utilization caused by acetoacetate (2 mM) in acini. Both glycerol and insulin reversed the increase in [citrate] and the decrease in [glycerol 3-phosphate] and the [lactate]/[pyruvate] ratio in the presence of acetoacetate. Lipogenesis from 3H2O, [3-14C] acetoacetate, [1-14C]- and [6-14C]-glucose was stimulated, whereas 14CO2 formation from [3-14C]acetoacetate was decreased. Neither insulin nor glycerol relieved the acetoacetate inhibition of glucose uptake when lipogenesis was inhibited by 5-(tetradecyloxy)-2-furoic acid. From measurements of [3-14C]acetoacetate incorporation into lipid in the various situations it is suggested that a cytosolic pathway for acetoacetate utilization may exist in rat mammary gland. In the absence of acetoacetate, glycerol inhibited glucose utilization by 60% and increased both [glycerol 3-phosphate] and the [lactate/[pyruvate] ratio. Possible ways in which glycerol may mimic the effects of insulin are discussed.

scholarly journals Red blood cell glucose metabolism in trisomy 10p: possible role of hexokinase in the erythrocyte

Blood ◽  
1983 ◽  
Vol 61 (5) ◽  
pp. 915-919
Author(s):  
M Magnani ◽  
V Stocchi ◽  
E Piatti ◽  
M Dacha ◽  
B Dallapiccola ◽  
...  
Keyword(s):  
Glucose Metabolism ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Glucose Utilization ◽  
De Novo ◽  
Dihydroxyacetone Phosphate ◽  
Cell Fractions ◽  
Cell Glucose ◽  
Normal Controls

Red blood cell glucose metabolism was investigated in a male patient with de novo trisomy 10p. According to previous evidence, when assigning hexokinase gene locus in the 10p11 leads to pter region, a triplex dosage effect of hexokinase activity (HK) was found, while all the other erythrocyte glycolytic enzymes were in the normal values range. Red blood cell glucose utilization was 2.87 mumole/hr/ml RBC as compared to 1.43 in normal controls; the rate of glucose metabolized through the hexose monophosphate shunt (HMPS) was unchanged. Glucose-6- phosphate, fructose-6-phosphate, fructose-1,6-diphosphate, and dihydroxyacetone phosphate increased with respect to normal controls, while normal levels of 3-phosphoglycerate, 2-phosphoglycerate, phosphoenolpyruvate, and ATP were found. The HK activity increased in all the red blood cell fractions obtained by density gradient ultracentrifugation. However, a small difference in the distribution of cells through the gradient was evident. The experiments reported in this article show that in the red blood cells of patients with trisomy 10p, an increased level of HK leads to higher concentrations of glucose- 6-phosphate and to a faster glucose utilization in the Embden-Meyerhof pathway, while the HMPS rate is unchanged.

scholarly journals Red blood cell glucose metabolism in trisomy 10p: possible role of hexokinase in the erythrocyte

Blood ◽  
1983 ◽  
Vol 61 (5) ◽  
pp. 915-919 ◽  
Author(s):  
M Magnani ◽  
V Stocchi ◽  
E Piatti ◽  
M Dacha ◽  
B Dallapiccola ◽  
...  
Keyword(s):  
Glucose Metabolism ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Glucose Utilization ◽  
De Novo ◽  
Dihydroxyacetone Phosphate ◽  
Cell Fractions ◽  
Cell Glucose ◽  
Normal Controls

Abstract Red blood cell glucose metabolism was investigated in a male patient with de novo trisomy 10p. According to previous evidence, when assigning hexokinase gene locus in the 10p11 leads to pter region, a triplex dosage effect of hexokinase activity (HK) was found, while all the other erythrocyte glycolytic enzymes were in the normal values range. Red blood cell glucose utilization was 2.87 mumole/hr/ml RBC as compared to 1.43 in normal controls; the rate of glucose metabolized through the hexose monophosphate shunt (HMPS) was unchanged. Glucose-6- phosphate, fructose-6-phosphate, fructose-1,6-diphosphate, and dihydroxyacetone phosphate increased with respect to normal controls, while normal levels of 3-phosphoglycerate, 2-phosphoglycerate, phosphoenolpyruvate, and ATP were found. The HK activity increased in all the red blood cell fractions obtained by density gradient ultracentrifugation. However, a small difference in the distribution of cells through the gradient was evident. The experiments reported in this article show that in the red blood cells of patients with trisomy 10p, an increased level of HK leads to higher concentrations of glucose- 6-phosphate and to a faster glucose utilization in the Embden-Meyerhof pathway, while the HMPS rate is unchanged.

scholarly journals Rapid inhibition of lipogenesis in vivo in lactating rat mammary gland by medium- or long-chain triacylglycerols and partial reversal by insulin

1980 ◽  
Vol 192 (1) ◽  
pp. 361-364 ◽  
Author(s):  
L Agius ◽  
D H Williamson
Keyword(s):  
Mammary Gland ◽  
Glucose Utilization ◽  
Insulin Administration ◽  
Hepatic Lipogenesis ◽  
Medium Chain ◽  
Rat Mammary Gland ◽  
Long Chain ◽  
Partial Reversal

An intragastric load of medium- or long-chain triacylglycerols inhibited lipogenesis in lactating rat mammary gland in vivo by 82 or 89% respectively. This inhibition was reversed partially by insulin administration. Long-chain triacylglycerols inhibited hepatic lipogenesis in vivo but medium-chain triacylglycerols increased it 2-fold. Glucose utilization in vitro by mammary gland acini from triacylglycerol-fed rat was normal.

The role of insulin in the regulation of AMP-activated protein kinase in lactating rat mammary gland

1992 ◽  
Vol 20 (3) ◽  
pp. 306S-306S ◽  
Author(s):  
MIHAJLO R. MILIC ◽  
KAREN A. OTTEY ◽  
SARVJINDER TAKHAR ◽  
MICHAEL R. MUNDAY
Keyword(s):  
Mammary Gland ◽  
Protein Kinase ◽  
Rat Mammary Gland ◽  
Amp Activated Protein Kinase
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