scholarly journals Biologically active macromolecular forms of oxytocin. [8-Lysine]oxytocin as a suitable ligand

1977 ◽  
Vol 165 (1) ◽  
pp. 43-47 ◽  
Author(s):  
C R Snell ◽  
D G Smyth
Keyword(s):  
Affinity Chromatography ◽  
Binding Proteins ◽  
Solid Support ◽  
Water Soluble ◽  
Biologically Active ◽  
Carboxyl Groups ◽  
Amino Group ◽  
Rat Uterus ◽  
Isolated Rat

[8-Lysine]oxytocin was synthesized on a solid support and possessed an oxytocic activity of 100 +/- 6 units mumol on the isolated rat uterus. The epsilon-carbamoyl, epsilon-3-carboxypropionyl and epsilon-3-carboxybutryl derivatives were prepared and had uterotonic activities of 400, 55 and 50 units/mumol respectively. [8-Lysine]oxytocin was coupled unambiguously through the epsilon-amino group to the carboxyl groups of carboxymethylated dextrans or epsilon-3-carboxypropionly-gelatin. The macromolecular oxytocins were water-soluble and retained signigicant oxytocic activity. [8-Lysine]oxytocin should prove a useful ligand for affinity chromatography of oxytocin-binding proteins.

FULLERENECARBOXYLIC ACIDS AND PROSPECTS FOR THEIR USE IN THE CHEMISTRY OF DRUGS

2018 ◽  
Vol 18 (2) ◽  
pp. 19-28
Author(s):  
E V Litasova ◽  
L B Piotrovskiy
Keyword(s):  
Delivery Systems ◽  
Water Soluble ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Carboxyl Groups ◽  
Derivatives Of ◽  
Active Substances

Among the various derivatives of fullerenes, compounds containing carboxyl groups (carboxyfullerenes) occupy a prominent place. The reason is that the introduction of carboxyl groups makes it possible to obtain water-soluble derivatives of highly lipophilic fullerenes. The review deals with matters of synthesis and structure of carboxyfullerenes, the influence of the amount and structure of the addends on the solubility of the entire compound, various aspects of the usage of these compounds as independent biologically active substances and carriers for creation of various delivery systems.

Synthesis of poly[N5-(2-hydroxyethyl)-L-glutamines] with modified side chain

1989 ◽  
Vol 54 (6) ◽  
pp. 1640-1647 ◽  
Author(s):  
Jindřich Pytela ◽  
František Rypáček ◽  
Marie Metalová ◽  
Jaroslav Drobník
Keyword(s):  
Benzyl Ester ◽  
Water Soluble ◽  
Biologically Active ◽  
Side Chain ◽  
Carboxyl Groups ◽  
Side Chains ◽  
Active Compounds ◽  
Water Soluble Polymers ◽  
Soluble Polymers ◽  
Step Procedure

The procedure for synthesis of water-soluble polymers on the basis of poly[N5-(2-hydroxyethyl)-L-glutamine] was developed. It allows binding of various groups of interest (reactive spacers, biologically active compounds, tracers etc.) to these polymers using side chains modifications. A multi-step procedure involves partial debenzylation of poly(γ-benzyl-L-glutamate), conversion of resulting carboxyl groups to reactive succinimido ester groups, which in turn are aminolyzed by the compound of interest. The final step of the synthesis, viz., aminolysis of residual benzyl ester groups with 2-aminoethanol, leads to water-soluble modified poly[N5-(2-hydroxyethyl)-L-glutamines].

HEMMUNG DER UTERUSWIRKUNG VON OXYTOCIN DURCH WASSERLÖSLICHE STEROIDE

1965 ◽  
Vol 48 (4) ◽  
pp. 656-663 ◽  
Author(s):  
R. Hempel ◽  
F. Neumann
Keyword(s):  
Barium Chloride ◽  
Water Soluble ◽  
Rat Uterus ◽  
Per Se ◽  
Isolated Rat ◽  
Spontaneous Rhythm

ABSTRACT Various water-soluble steroids were tested in vitro on the isolated rat uterus for their oxytocin-antagonistic activities. Using progestional agents (19-nor-17α-hydroxyprogesterone-17-hemisulfate-sodium, Δ1,6-17α-hydroxyprogesterone-17-hemisulfate-sodium) oestrogens (oestrone-hemisulfate-sodium), and corticoids (dexamethasone-21-hemisulfate-sodium) it was possible to suppress both the oxytocin-induced single contraction and the spontaneous rhythm intensified by oxytocin. Except for the Δ1,6-17α-hydroxyprogesterone-sulfate-sodium, these compounds were unable to inhibit the single contractions of the uterus induced by serotonin, bradykinin and barium chloride. Following the administration of a mixture of the different watersoluble steroids an additional effect was observed. Based on these results, it can be assumed that the oxytocinantagonistic effect represents an activity per se which is peculiar to certain steroid structures.

The content of decenoic acids in drone brood preparations and combined preparations based on it

Biomics ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 389-393
Author(s):  
D.V. Mitrofanov ◽  
N.V. Budnikova
Keyword(s):  
Royal Jelly ◽  
Storage Conditions ◽  
Water Soluble ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Strict Adherence ◽  
Drone Brood ◽  
Wide Range ◽  
Active Substances ◽  
Melanin Complex

The drone brood contains a large number of substances with antioxidant activity. These substances require stabilization and strict adherence to storage conditions. Among these substances are unique decenoic acids, the content of which is an indicator of the quality of drone brood and products based on it. The ability of drone brood to reduce the manifestations of oxidative stress is shown. There are dietary supplements for food and drugs based on drone brood, which are used for a wide range of diseases. Together with drone brood, chitosan-containing products, propolis, royal jelly can be used. They enrich the composition with their own biologically active substances and affect the preservation of the biologically active substances of the drone brood. Promising are the products containing, in addition to the drone brood, a chitin-chitosan-melanin complex from bees, propolis, royal jelly. The chitin-chitosan-melanin complex in the amount of 5% in the composition of the adsorbent practically does not affect the preservation of decenic acids, while in the amount of 2% and 10% it somewhat worsens. The acid-soluble and water-soluble chitosan of marine crustaceans significantly worsens the preservation of decenoic acids in the product. Drone brood with royal jelly demonstrates a rather high content of decenoic acids. When propolis is introduced into the composition of the product, the content of decenoic acids increases according to the content of propolis.

scholarly journals Preparation of biologically active 32P-labeled human relaxin. Displaceable binding to rat uterus, cervix, and brain.

1990 ◽  
Vol 265 (16) ◽  
pp. 9396-9401
Author(s):  
P L Osheroff ◽  
V T Ling ◽  
R L Vandlen ◽  
M J Cronin ◽  
J A Lofgren
Keyword(s):  
Biologically Active ◽  
Rat Uterus

scholarly journals Solid Dispersions of Anthelmintics and Plant Protection Preparations

Solids ◽  
2021 ◽  
Vol 2 (1) ◽  
pp. 60-75
Author(s):  
Salavat S. Khalikov
Keyword(s):  
Plant Protection ◽  
Rapid Development ◽  
Solid Dispersions ◽  
Animal Health ◽  
Water Soluble ◽  
Biologically Active ◽  
Cultivated Plants ◽  
Industrial Complex ◽  
Scientific Papers ◽  
Poorly Soluble

Because of the rapid development of nanotechnologies, materials, in particular, solid dispersions (SDs), which are actively introduced into the life of modern man, have been obtained. Special progress in this area is observed in industry and medicine. The use of SDs in agriculture is lagging far behind, despite the growing number of scientific papers on this topic. At the same time, the prospects for the introduction of SDs in the agro-industrial complex are obvious. The review presents the results of research on the development of innovative preparations based on SD to protect plants from diseases and pests of cultivated plants, as well as parasiticides to protect animal health based on modern achievements of nanotechnology. One of these technologies is the methods of mechanochemistry, which improve the properties of poorly soluble biologically active substances by their joint mechanical treatment with water-soluble polymers and auxiliary substances.

Identification of a C/EBP-Rel complex in avian lymphoid cells

1994 ◽  
Vol 14 (10) ◽  
pp. 6635-6646
Author(s):  
J A Diehl ◽  
M Hannink
Keyword(s):  
Gene Expression ◽  
Affinity Chromatography ◽  
Protein Interactions ◽  
Binding Proteins ◽  
Regulation Of Gene Expression ◽  
Lymphoid Cells ◽  
Cytokine Gene Expression ◽  
Protein Protein Interactions ◽  
Dna Complex ◽  
Dna Affinity Chromatography

Protein-protein interactions between the CCAAT box enhancer-binding proteins (C/EBP) and the Rel family of transcription factors have been implicated in the regulation of cytokine gene expression. We have used sequence-specific DNA affinity chromatography to purify a complex from avian T cells that binds to a consensus C/EBP motif. Our results provide evidence that Rel-related proteins are components of the C/EBP-DNA complex as a result of protein-protein interactions with the C/EBP proteins. A polyclonal antiserum raised against the Rel homology domain of v-Rel and antisera raised against two human RelA-derived peptides specifically induced a supershift of the C/EBP-DNA complex in mobility shift assays using the affinity-purified C/EBP. In addition, several kappa B-binding proteins copurified with the avian C/EBP complex through two rounds of sequence-specific DNA affinity chromatography. The kappa B-binding proteins are distinct from the C/EBP proteins that directly contact DNA containing the C/EBP binding site. The identification of a protein complex that binds specifically to a consensus C/EBP site and contains both C/EBP and Rel family members suggests a novel mechanism for regulation of gene expression by Rel family proteins.

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