scholarly journals Proton translocation coupled to electron flow from endogenous substrates to fumarate in anaerobically grown Escherichia coli K12

1977 ◽  
Vol 164 (1) ◽  
pp. 265-267 ◽  
Author(s):  
S J Gutowski ◽  
H Rosenberg
Keyword(s):  
Escherichia Coli ◽  
Electron Flow ◽  
Proton Translocation ◽  
Optimum Conditions ◽  
Escherichia Coli K12 ◽  
Minimum Value ◽  
Endogenous Substrates

Observed leads to H+/2e- values for proton translocation during the reduction of fumarate by endogenous substrates in anaerobic cells of Escherichia coli K12 varied with fumarate concentration. This variation was probably due mainly to incomplete fumarate utilization. Under optimum conditions a minimum value for leads to H+/2e- of 1.04+/-0.20 was obtained.

scholarly journals Effects of dicyclohexylcarbodi-imide on proton translocation coupled to fumarate reduction in anaerobically grown cells of Escherichia coli K-12

1976 ◽  
Vol 160 (3) ◽  
pp. 813-816 ◽  
Author(s):  
S J Gutowski ◽  
H Rosenberg
Keyword(s):  
Escherichia Coli ◽  
Reductase Activity ◽  
Proton Translocation ◽  
Fumarate Reductase ◽  
Escherichia Coli K12 ◽  
Fumarate Reduction ◽  
Cell Extracts ◽  
Endogenous Substrates ◽  
K 12

The addition of dicyclohexylcarbodi-imide to anaerobic cells of Escherichia coli K12 decreases both the observed extent of proton translocation coupled to fumarate reduction by endogenous substrates and the t1/2 of proton re-entry after such translocation, but does not affect fumarate uptake. Dicyclohexylcarbodi-imide also inhibits fumarate reductase activity in cell extracts.

Mutations that alter the pore function of the ompF porin of Escherichia coli K12

1988 ◽  
Vol 203 (4) ◽  
pp. 961-970 ◽  
Author(s):  
S.A. Benson ◽  
J.L.L. Occi ◽  
B.A. Sampson
Keyword(s):  
Escherichia Coli ◽  
Ompf Porin ◽  
Escherichia Coli K12

Factors which influence CaCl2 dependent transfection of Lambda DNA in Escherichia coli K12 recipients

1980 ◽  
Vol 20 (4) ◽  
pp. 271-281 ◽  
Author(s):  
M. Pfeifer ◽  
Ch. Pöhlmann ◽  
A. Betcke ◽  
M. Kurth ◽  
D.-H. Liebscher
Keyword(s):  
Escherichia Coli ◽  
Escherichia Coli K12 ◽  
Lambda Dna
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