scholarly journals Biospecific-elution chromatography with ‘imphilytes’ as stationary phases

1977 ◽  
Vol 161 (2) ◽  
pp. 233-237 ◽  
Author(s):  
R J Yon
Keyword(s):  
Affinity Chromatography ◽  
Enzyme Purification ◽  
Stationary Phases ◽  
Chromatographic Behaviour ◽  
Presence And Absence ◽  
Elution Chromatography

Six out of seven enzymes tested (four of them nicotinamide nucleotide-dependent dehydrogenases) showed differences in chromatographic behaviour in the presence and absence of their biospecific ligands, when chromatographed on immobilized amphipathic ampholytes (‘imphilytes’) as stationary phases. Some enzymes were adsorbed more tightly, others less tightly, in the presence of ligands. These results have implications for enzyme purification in general, and for some types of affinity chromatography in particular.

Principles of Multi-Enzyme Purification by Affinity Chromatography

1978 ◽  
pp. 441-442 ◽  
Author(s):  
C.-Y. Lee ◽  
A. Leigh-Brown ◽  
C. Langley ◽  
B. Pegoraro ◽  
J. Lopez-Barea ◽  
...  
Keyword(s):  
Affinity Chromatography ◽  
Enzyme Purification

Synthese von immobilisierten Peptidfragmenten an Polystyrol-Polyoxyethylen zur Affinitätschromatographie /Synthesis of Immobilized Peptide Fragments on Polystyrene-Polyoxyethylene for Affinity Chromatography

1987 ◽  
Vol 42 (4) ◽  
pp. 455-460 ◽  
Author(s):  
Ernst Bayer ◽  
Heribert Hellstern ◽  
Heiner Eckstein
Keyword(s):  
Affinity Chromatography ◽  
Organic Solvents ◽  
Peptide Synthesis ◽  
Stationary Phases ◽  
Peptide Bond ◽  
Protecting Groups ◽  
Peptide Fragments ◽  
Solid Supports ◽  
Polystyrene Beads ◽  
Acidic Conditions

Abstract Polystyrene-polyoxyethylene craft copolymers have been used for step-wise peptide synthesis. After completion of synthesis the protecting groups are cleaved under acidic conditions, where the polymer-peptide bond is stable. These gels in comparison to polystyrene peptide gels, show better properties for applications in affinity chromatography as well as synthesis on solid supports, because the advantageous properties of polystyrene beads are combined with the excellent spacer behavior of polyoxyethylene chains (mobility, solvation by water and organic solvents). Peptide gels with polylysine sequences have been synthesized as highly selective stationary phases for the separation of the homologous oligo desoxyribonucleotides (dT)n with n = 1-3. The principal possibilities of these gels for affinity chromatography is demonstrated.

ISOLATION AND IDENTIFICATION OF 20α-HYDROXY-5β-PREGNAN-3-ONE FROM URINE OF PREGNANT WOMEN: GAS CHROMATOGRAPHY OF PREGNANOLONES AND PREGNANEDIOLS

1968 ◽  
Vol 42 (1) ◽  
pp. 45-54 ◽  
Author(s):  
H. J. van der MOLEN ◽  
D. GROEN ◽  
F. F. G. ROMMERTS ◽  
J. H. VAN DER MAAS
Keyword(s):  
Gas Chromatography ◽  
Pregnant Women ◽  
Stationary Phases ◽  
Infrared Spectrometry ◽  
Isolation And Identification ◽  
Chromatographic Behaviour ◽  
Pregnancy Urine

SUMMARY 20α-Hydroxy-5β-pregnan-3-one was isolated from the extracts of enzyme or acid-hydrolysed pregnancy urine using various chromatographic procedures including gas chromatography Identification of the steroid was based upon: (1) behaviour of the free and acetylated compound, and of the products, following stereospecific enzymatic oxidations, during gas chromatography on different stationary phases, and (2) infrared spectrometry of the compound collected after gas chromatography. The gas-chromatographic behaviour of the isomers of 3-hydroxypregnan-20-one, 20-hydroxypregnan3-one and pregnane-3,20-diol and their acetates is reported.

Immobilized metal-ion affinity chromatography of peptides on metalloporphyrin stationary phases

1999 ◽  
Vol 364 (1-2) ◽  
pp. 160-164 ◽  
Author(s):  
Magdalena Biesaga ◽  
Jowita Orska ◽  
Dariusz Fiertek ◽  
J. Izdebski ◽  
M. Trojanowicz
Keyword(s):  
Affinity Chromatography ◽  
Metal Ion ◽  
Stationary Phases ◽  
Metal Ion Affinity Chromatography

New Conducting Polymer Affinity Chromatography Stationary Phases

1990 ◽  
Vol 13 (15) ◽  
pp. 3091-3110 ◽  
Author(s):  
G. G. Wallace ◽  
K. E. Maxwell ◽  
T. W. Lewis ◽  
A. J. Hodgson ◽  
M. J. Spencer
Keyword(s):  
Affinity Chromatography ◽  
Conducting Polymer ◽  
Stationary Phases

Chromatographic behaviour and comparison of column packed with sub-2μm stationary phases in liquid chromatography

2006 ◽  
Vol 1128 (1-2) ◽  
pp. 105-113 ◽  
Author(s):  
Dao T.-T. Nguyen ◽  
Davy Guillarme ◽  
Serge Rudaz ◽  
Jean-Luc Veuthey
Keyword(s):  
Liquid Chromatography ◽  
Stationary Phases ◽  
Chromatographic Behaviour
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