Glycosylation in vitro of an asparagine sequon catalysed by preparations of yeast cell membranes
Keyword(s):
Preparations of yeast cell membranes can catalyse in vitro the N-acetyl-beta-D-glucosaminylation of the asparagine sequon at residues 34-36 of bovine pancreatic ribonuclease A. The relevant glycopeptides were isolated from tryptic hydrolysates of the glycosylated ribonuclease and analysed. The donor used was UDP-N-acetyl-D-glucosamine, although the mechanism of the transfer is unknown. Mn2+ ions at concentrations of 25 mM double the activity of the enzymic transfer.
1963 ◽
Vol 238
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pp. 618-621
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1973 ◽
Vol 248
(22)
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pp. 7833-7837
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1997 ◽
Vol 231
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pp. 671-674
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2011 ◽
Vol 319
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pp. 49-56
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1971 ◽
Vol 146
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pp. 513-524
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