scholarly journals Interaction of pigeon-liver nicotinamide-adenine dinucleotide kinase with cibacron blue F3GA

1976 ◽  
Vol 159 (2) ◽  
pp. 441-443 ◽  
Author(s):  
D K Apps ◽  
C D Gleed
Keyword(s):  
Nicotinamide Adenine Dinucleotide ◽  
Spectrophotometric Titration ◽  
Nad Kinase ◽  
Binding Region ◽  
Cibacron Blue ◽  
Steady State Rate ◽  
State Rate ◽  
A Site ◽  
Cibacron Blue F3ga ◽  
Pigeon Liver

The interaction of pigeon liver NAD kinase with Cibacron Blue F3GA was investigated. By using steady-state rate measurements, spectrophotometric titration and chromatography of the enzyme on immobilized dye, it was shown that binding occurs at two nucleotide sites with different affinities, and also at a site distinct from the substrate-binding region.

scholarly journals Preparation and properties of nylon-tube-supported nicotinamide--adenine dinucleotide kinase

1977 ◽  
Vol 161 (2) ◽  
pp. 441-443 ◽  
Author(s):  
P V Sundaram ◽  
D K Apps
Keyword(s):  
Nicotinamide Adenine Dinucleotide ◽  
Ph Dependence ◽  
Nicotinamide Adenine ◽  
Nad Kinase ◽  
Nylon Tube ◽  
Pigeon Liver

Pigeon liver NAD kinase was covalently coupled to the inside surfaces of nylon tubes, both directly to the nylon and via polyamine specers Km values and the inactivation energy of the reaction changed upon immobilization, but the pH-dependence remained unaltered. The activities and stabilities of different preparations are compared.

scholarly journals Amplification characteristics in active tapered segmented cladding fiber with large mode area

2021 ◽  
Vol 9 ◽  
Author(s):  
Caijian Xie ◽  
Tigang Ning ◽  
Jingjing Zheng ◽  
Li Pei ◽  
Jianshuai Wang ◽  
...  
Keyword(s):  
High Power ◽  
Heat Load ◽  
Nonlinear Effects ◽  
Single Mode ◽  
Large Mode Area ◽  
High Loss ◽  
Effective Mode Area ◽  
Steady State Rate ◽  
State Rate ◽  
Mode Area

Abstract A kind of tapered segmented cladding fiber (T-SCF) with large mode area (LMA) is proposed, and the mode and amplification characteristics of T-SCFs with concave, linear, and convex tapered structures are investigated based on finite-element method (FEM) and few-mode steady-state rate equation. Simulation results indicate that the concave tapered structure can introduce high loss for high-order modes (HOMs) that is advantageous to achieve single-mode operation, whereas the convex tapered structure provides large effective mode area that can help to mitigate nonlinear effects. Meanwhile, the small-to-large amplification scheme shows further advantages on stripping off HOMs, and the large-to-small amplification scheme decreases the heat load density induced by the high-power pump. Moreover, single-mode propagation performance, effective mode area, and heat load density of the T-SCF are superior to those of tapered step index fiber (T-SIF). These theoretical model and numerical results can provide instructive suggestions for designing high-power fiber lasers and amplifiers.

Binding of human interferons to immobilized Cibacron Blue F3GA: the nature of molecular interaction

Biochemistry ◽  
1976 ◽  
Vol 15 (23) ◽  
pp. 5182-5187 ◽  
Author(s):  
Wieslaw J. Jankowski ◽  
Wittiches Von Muenchhausen ◽  
Eugene Sulkowski ◽  
William A. Carter
Keyword(s):  
Molecular Interaction ◽  
Cibacron Blue ◽  
Cibacron Blue F3ga

Corticosterone secretion in rats as a function of ACTH input and adrenal blood flow

1965 ◽  
Vol 209 (4) ◽  
pp. 811-814 ◽  
Author(s):  
John C. Porter ◽  
M. S. Klaiber
Keyword(s):  
Blood Flow ◽  
Steady State ◽  
Steady Increase ◽  
Constant Input ◽  
Corticosterone Secretion ◽  
Steady State Rate ◽  
State Rate

The rate of secretion of corticosterone from the left adrenal of rats receiving a constant input of ACTH was determined for different flows of blood through the adrenal during the 2- to 3-hr interval following hypophysectomy. Two hours after hypophysectomy the secretion of corticosterone was low in all groups regardless of flow. An input of 0.26 mU ACTH/min caused a steady increase in secretion for 30–40 min before a steady-state rate was attained. The average steady-state rate of secretion was 1.1, 2.4, 3.5, 6.2, 7.2, 6.2, and 6.2 µg/5 min for flows of 0.005, 0.012, 0.023, 0.034, 0.039, 0.051, and 0.058 ml/min, respectively. Under the conditions of these experiments where the input of ACTH was 0.26 mU/min the secretion of corticosterone increased significantly with time of input of ACTH and with flow of blood through the adrenal.

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