scholarly journals Kinetics of rat liver glucokinase. Co-operative interactions with glucose at physiologically significant concentrations

1976 ◽  
Vol 159 (1) ◽  
pp. 7-14 ◽  
Author(s):  
A C Storer ◽  
A Cornish-Bowden
Keyword(s):  
Rat Liver ◽  
Glucose Concentration ◽  
Initial Rate ◽  
Constant Concentration ◽  
Hill Coefficients ◽  
Kinetics Of

The kinetics of glucokinase from rat liver were studied over wide ranges of glucose and MgATP2- concentrations. The initial rate shows a co-operative dependence on the glucose concentration, with Hill coefficients in the range 1.2-1.5. The degree of glucose co-operativity increases with the MgATP2- concentration, but no co-operativity was detected for the dependence of the rate on the MgATP2- concentration. The effects observed occur at physiologically reasonable concentrations of glucose and MgATP2- and are consistent with the presumed function of glucokinase in maintaining a constant concentration of glucose in the blood.

VANILLINSÄURE ALS ENDPRODUKT DES STOFFWECHSELS VON ADRENALIN UND NORADRENALIN. II.

1963 ◽  
Vol 44 (1) ◽  
pp. 101-106 ◽  
Author(s):  
Wilhelm Dirscherl ◽  
Helmut Thomas
Keyword(s):  
Rat Liver ◽  
Paper Chromatography ◽  
Column Chromatography ◽  
Vanillic Acid ◽  
Hippuric Acid ◽  
Single Spot ◽  
Solvent Systems ◽  
Perfusion Experiment ◽  
Kinetics Of

ABSTRACT Perfusion of rat liver with vanillic acid yielded only one metabolite. In paper chromatography with three different solvent systems, the substance showed the same RF-values as vanillyolglycine (3-methoxy-4-hydroxyhippuric acid) and in mixed chromatograms there was only one single spot. After separation by column chromatography, the UV- and IRspectra of the reaction product were identical with those of 3-methoxy4-hydroxy-hippuric acid. During the perfusion experiment, the kinetics of the conjugation were investigated.

Kinetics of oestradiol-dependent induction of the prolactin receptor in rat liver

1988 ◽  
Vol 117 (4_Suppl) ◽  
pp. S88
Author(s):  
R. GÜNTHER ◽  
H. C. BLOSSEY
Keyword(s):  
Rat Liver ◽  
Prolactin Receptor ◽  
Kinetics Of

The effect of electrondonors on the polymerization kinetics of isoprene

1980 ◽  
Vol 45 (12) ◽  
pp. 3338-3346
Author(s):  
Miroslav Kašpar ◽  
Jiří Trekoval
Keyword(s):  
Induction Period ◽  
Ethyl Ether ◽  
Reaction Order ◽  
Propagation Rate ◽  
Reaction Scheme ◽  
Polymerization Kinetics ◽  
Polymerization Rate ◽  
Constant Concentration ◽  
Initial Concentration ◽  
Kinetics Of

The effect of small additions of 1-octene, butyl ethyl ether and triethylamine on the polymerization kinetics of isoprene (2-methyl-1,3-butadiene) in benzene initiated with butyllithium was investigated by employing the GLC analysis. The addition of 1-octane was reflected only in a shorter induction period of the reaction; the effect on the propagation rate was insignificant. With the increasing amount of butyl ethyl ether, the polymerization rate increases linearly, while the reaction order with respect to the concentration of triethylamine is variable and increases from 0.33 to 0.66 with the increasing concentration of the initiator. For a constant concentration of triethylamine, the reaction order with respect to the initial concentration of the initiator was found to vary considerably, reaching even negative values. A reaction scheme was suggested, taking into account the competition between two different solvates of alkyllithium.

scholarly journals Thermodynamic and detailed kinetic study of the formation of orthophthalaldehyde-agmatine complex by fluorescence intensities

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Khémesse Kital ◽  
Moumouny Traoré ◽  
Diégane Sarr ◽  
Moussa Mbaye ◽  
Mame Diabou Gaye Seye ◽  
...  
Keyword(s):  
Complex Formation ◽  
Thermodynamic Parameters ◽  
Initial Rate ◽  
Reaction Medium ◽  
Standard Entropy ◽  
Step Size ◽  
Formation Reaction ◽  
Different Temperatures ◽  
Kinetics Of ◽  
Complex Formation Process

Abstract The aim of this work is to determine the thermodynamic parameters and the kinetics of complex formation between orthophthalaldehyde (OPA) and agmatine (AGM) in an alkaline medium (pH 13). Firstly, the association constant (Ka) between orthophthalaldehyde and agmatine was determined at different temperatures (between 298 K and 338 K) with a step size of 10 K. Secondly, the thermodynamic parameters such as standard enthalpy (ΔH°), standard entropy (ΔS°),and Gibbs energy (∆G) were calculated, where a positive value of ΔH° (+45.50 kJ/mol) was found, which shows that the reaction is endothermic. In addition, the low value of ΔS°(+0.24 kJ/mol) indicates a slight increase in the disorder in the reaction medium. Furthermore, the negative values of ΔG between −35.62 kJ/mol and −26.02 kJ/mol show that the complex formation process is spontaneous. Finally, the parameters of the kinetics of the reaction between OPA and AGM were determined as follows: when the initial concentration of AGM (5 × 10−6 M) is equal to that of the OPA, the results show that the reaction follows an overall 1.5 order kinetics with an initial rate of 5.1 × 10−7Mmin−1 and a half-life of 8.12 min. The partial order found in relation to the AGM is 0.8. This work shows that the excess of OPA accelerates the formation reaction of the complex.

Kinetics of sulfation and glucuronidation of harmol in the perfused rat liver preparation

1982 ◽  
Vol 31 (19) ◽  
pp. 3023-3028 ◽  
Author(s):  
Henk Koster ◽  
Ina Halsema ◽  
Egbert Scholtens ◽  
K.Sandy Pang ◽  
Gerard J. Mulder
Keyword(s):  
Rat Liver ◽  
Perfused Rat Liver ◽  
Liver Preparation ◽  
Kinetics Of

The mechanism of the oxidation of thiocyanate ion by peroxomonosulphate in aqueous solution. II. Kinetics of the reaction

1966 ◽  
Vol 19 (8) ◽  
pp. 1365 ◽  
Author(s):  
RH Smith ◽  
IR Wilson
Keyword(s):  
Aqueous Solution ◽  
Initial Rate ◽  
Stopped Flow ◽  
Thiocyanate Ion ◽  
First Order ◽  
Conductance Method ◽  
Rate Of Reaction ◽  
Kinetics Of

Initial rates of reaction for the above oxidation have been measured by a stopped-flow conductance method. Between pH 2 and 3.6, the initial rate of reaction, R, is given by the expression R{[HSO5-]+[SCN-]} = {kb+kc[H+]}[HSO5-]0[SCN-]20+ka[H+]-1[HSO5]20[SCN-]0 As pH increases, there is a transition to a pH-independent rate, first order in each thiocyanate and peroxomonosulphate concentrations.

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