Protein phosphorylation in respiring slices of guinea-pig cerebral cortex. Evidence for a role for noradrenaline and adenosine 3′:5′-cyclic monophosphate in the increased phosphorylation observed on application of electrical pulses

1976 ◽  
Vol 158 (3) ◽  
pp. 513.b1-513.b1
Author(s):  
M. Williams ◽  
R. Rodnight
Keyword(s):  
Cerebral Cortex ◽  
Guinea Pig ◽  
Protein Phosphorylation ◽  
Cyclic Monophosphate ◽  
Electrical Pulses

scholarly journals Turnover of protein-bound serine phosphate in respiring slices of guinea-pig cerebral cortex. Effects of putative transmitters, tetrodotoxin and other agents

1973 ◽  
Vol 132 (3) ◽  
pp. 475-482 ◽  
Author(s):  
Martin Reddington ◽  
Richard Rodnight ◽  
Michael Williams
Keyword(s):  
Cerebral Cortex ◽  
Cyclic Amp ◽  
Guinea Pig ◽  
Electrical Pulses ◽  
Phosphorus Turnover ◽  
Serine Phosphate ◽  
Stimulation Of

1. The effect of various agents on the turnover of protein-bound phosphorus in respiring slices of cerebral cortex was studied. 2. Confirming previous work turnover was increased by the application of electrical pulses for 10s to the tissue. 3. Turnover was also increased by exposure of the slices for 10min to noradrenaline (0.5mm), 5-hydroxytryptamine (1μm) and histamine (0.1mm). 4. When slices were stimulated by electrical pulses in the presence of histamine the increase in turnover was the sum of the responses given by each agent above, suggesting that different phosphorylating systems were involved. 5. Tetrodotoxin (0.5μm) blocked the increased turnover due to electrical pulses, but not that due to histamine. Tetrodotoxin also prevented the increase in tissue cyclic AMP content caused by the application of electrical pulses. 6. Phosphoprotein turnover was not affected by adenosine, despite the increase in tissue cyclic AMP content given by this agent. 7. Adenosine blocked the phosphoprotein response to histamine, but did not affect the response to electrical pulses. 8. The results are discussed in relation to the hypothesis that the stimulation of protein phosphorus turnover by electrical pulses is secondary to the release of cyclic AMP in the tissue.

scholarly journals Protein phosphorylation in respiring slices of guinea-pig cerebral cortex. Evidence for a role for noradrenaline and adenosine 3′:5′-cyclic monophosphate in the increased phosphorylation observed on application of electrical pulses

1976 ◽  
Vol 154 (1) ◽  
pp. 163-170 ◽  
Author(s):  
M Williams ◽  
R Rodnight
Keyword(s):  
Cerebral Cortex ◽  
Adenylate Cyclase ◽  
Protein Phosphorylation ◽  
Adrenergic Receptor ◽  
Prostaglandin E1 ◽  
Experimental Conditions ◽  
Blocking Agents ◽  
Electrical Pulses ◽  
Adrenergic Blocking ◽  
Adrenergic Blocking Agents

1. Exposure of slices of cerebral cortex from guinea pigs to electrical pulses for 10s or to noradrenaline, 5-hydroxytryptamine or histamine increases the rate of phosphorylation of unidentified proteins in the tissue; the increases in protein phosphorylation due to electrical pulses and noradrenaline were non-additive, whereas the increases due to pulses and 5-hydroxytryptamine or histamine were additive. 2. The stimulating effects of electrical pulses and noradrenaline on protein phosphorylation were antagonized by the ʲ-adrenergic blocking agents L-propranolol, dichloroisoprenaline, practolol and ICI 66082, but not by the ʱ-adrenergic blocking agents, phentolamine and phenoxybenzamine. 3. The increase in protein phosphorylation associated with electrical pulses was antagonized by 10 μm-trifluoperazine and 0.5 μm-prostaglandin E1. 4. It is postulated that under the experimental conditions used the action of electrical pulses on protein phosphorylation is mediated by noradrenaline acting through a ʲ-adrenergic receptor mechanism probably involving adenylate cyclase.

Effect of (-)nicotine on GABA efflux from guinea pig cerebral cortex slices

1989 ◽  
Vol 21 ◽  
pp. 133-134 ◽  
Author(s):  
P. Spalluto ◽  
M. Morari ◽  
L. Ferraro ◽  
A. Nordberg ◽  
T. Antonelli ◽  
...  
Keyword(s):  
Cerebral Cortex ◽  
Guinea Pig ◽  
Cortex Slices

Isolation of arteriolar microvessels and culture of smooth muscle cells from cerebral cortex of guinea pig

1991 ◽  
Vol 265 (3) ◽  
pp. 579-587 ◽  
Author(s):  
Matthias F. Seidel ◽  
J. Marc Simard ◽  
Samuel F. Hunter ◽  
Gerald A. Campbell
Keyword(s):  
Cerebral Cortex ◽  
Smooth Muscle ◽  
Guinea Pig ◽  
Smooth Muscle Cells ◽  
Muscle Cells

Lactic dehydrogenases of the developing cerebral cortex and liver of the mouse and guinea pig

1960 ◽  
Vol 2 (4) ◽  
pp. 313-328 ◽  
Author(s):  
L.B. Flexner ◽  
J.B. Flexner ◽  
R.B. Roberts ◽  
G. de la Haba
Keyword(s):  
Cerebral Cortex ◽  
Guinea Pig

Effect of ketamine/xylazine anaesthesia on 5-hydroxytryptamine uptake in guinea pig cerebral cortex

1990 ◽  
Vol 183 (4) ◽  
pp. 1389-1390
Author(s):  
C.D. Hornsby ◽  
J.M. Barnes ◽  
N.M. Barnes ◽  
B. Costall ◽  
R.J. Naylor
Keyword(s):  
Cerebral Cortex ◽  
Guinea Pig
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