scholarly journals The mechanism of sensitivity to phleomycin in growing Escherichia coli cells

1976 ◽  
Vol 155 (1) ◽  
pp. 87-99 ◽  
Author(s):  
M J Sleigh ◽  
G W Grigg
Keyword(s):  
Escherichia Coli ◽  
Dna Replication ◽  
Cell Membrane ◽  
Dna Breakage ◽  
Escherichia Coli Cells ◽  
Low Concentrations ◽  
Cellular Change ◽  
Initiation Of Dna Replication ◽  
Cellular Dna ◽  
Escherichia Coli B

Stationary-phase Escherichia coli B cells transferred to new growth medium are initially resistant to net DNA breakage by low concentrations of phleomycin, and become sensitive as DNA replication commences. From studies with inhibitors of various stages of the DNA replication cycle it is evident that it is not DNA synthesis itself that is required for induction of DNA breakage by phleomycin, but events associated with the initiation of DNA replication. Termination of replication in the absence of further initiaiton results in resistance to phleomycin. The cellular change responsible for changes in sensitivity to phleomycin could be the attachment of the bacterial chromosome to the cell membrane at initiation and detachment on termination of replication, suggesting an alteration in the balance between cellular DNA breakage and repair processes for membrane-associated compared with non-membrane-associated DNA.

scholarly journals Modification by an R determinant for streptomycin of hissd phenotype in a mutant strain of Escherichia coli B

1968 ◽  
Vol 12 (2) ◽  
pp. 109-116 ◽  
Author(s):  
A. M. Molina ◽  
L. Calegari ◽  
G. Conte
Keyword(s):  
Escherichia Coli ◽  
Cell Membrane ◽  
Mutant Strain ◽  
Minimal Medium ◽  
Specific Requirement ◽  
Resistance Level ◽  
E Coli ◽  
Level Characteristic ◽  
Escherichia Coli B

When an R determinant for streptomycin is transferred into a conditionally streptomycin-dependent E. coli B mutant—which requires in minimal medium either histidine or streptomycin—the latter behaves like a histidineless strain. This phenotype modification shows that the repairing action of streptomycin is prevented. The specific requirement of the strain is not now replaced even by streptomycin concentrations up to 10000 µg/ml at which the conditionally streptomycin-dependent mutant could originally grow, and which are well beyond the resistance level characteristic of the R determinant itself. These data seem to suggest that a reduction in permeability of the cell membrane cannot be held responsible for the phenomenon observed.

scholarly journals Toxin Kid uncouples DNA replication and cell division to enforce retention of plasmid R1 in Escherichia coli cells

2014 ◽  
Vol 111 (7) ◽  
pp. 2734-2739 ◽  
Author(s):  
B. Pimentel ◽  
R. Nair ◽  
C. Bermejo-Rodriguez ◽  
M. A. Preston ◽  
C. A. Agu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cell Division ◽  
Dna Replication ◽  
Plasmid R1 ◽  
Escherichia Coli Cells

Über die Zellmembran von Escherichia Coli B

1954 ◽  
Vol 9 (6) ◽  
pp. 398-406 ◽  
Author(s):  
Wolfhard Weidel ◽  
Gebhard Koch ◽  
Friedrich Lohss
Keyword(s):  
Escherichia Coli ◽  
Chemical Analysis ◽  
Cell Membrane ◽  
B Cell ◽  
Dry Weight ◽  
Shigella Sonnei ◽  
Receptor Activity ◽  
Escherichia Coli B

Dissociation in 90% phenol uncovers a layer of the Coli B-cell membrane showing the typical antiviral specificity of the receptor for phages T3, T4 and T7. Chemical analysis proved glucose, glucosamine and a yet unknown carbohydrate, probably a heptose, to be components of the receptor material, which amounts to about 13% of the dry weight of the whole membrane.Analogous material obtained from the membrane of B/3,4,7, a B-mutant resistant against phages T3, T4 and T7, has no activity against these phages and contains glucosamine and small amounts of glucose, but no heptose.In view of similar findings of Jesaitis and Goebel with T3,4,7 - receptor material from Shigella Sonnei, the rôle of the heptose as a characteristic and functionally indispensable component of lipocarbohydrates with receptor activity against T3, T4 and T7 is discussed.

scholarly journals Spontaneous DNA breakage in single living Escherichia coli cells

2007 ◽  
Vol 39 (6) ◽  
pp. 797-802 ◽  
Author(s):  
Jeanine M Pennington ◽  
Susan M Rosenberg
Keyword(s):  
Escherichia Coli ◽  
Dna Breakage ◽  
Escherichia Coli Cells ◽  
Single Living
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