scholarly journals A genetic marker in the variable region of rabbit immunoglobulin heavy chain

1975 ◽  
Vol 151 (2) ◽  
pp. 351-359 ◽  
Author(s):  
L R Mole
Keyword(s):  
Genetic Marker ◽  
Heavy Chain ◽  
Variable Region ◽  
Immunoglobulin Heavy Chain ◽  
Heavy Chains ◽  
Rabbit Immunoglobulin ◽  
V Region ◽  
Peptide Map ◽  
Relationship Of ◽  
The Relationship

A previous study [Mole et al. (1971) Biochem. J. 124, 301-318] showed several differences in sequence between the variable (V) regions of rabbit immunoglobulin Aa1 and Aa3 heavy chains. The inheritance of one such difference has been followed in a family of 38 rabbits by a radioautographic peptide-‘map’ technique and is shown to segregate in a Mendelian fashion. This clearly demonstrates the presence of a genetic marker in the rabbit heavy-chain V region, although the finding that Aa2 and Aa3 heavy chains have identity of sequence in the region studied obscures the relationship of this genetic marker to the a locus.

scholarly journals Allotypically related sequences in the Fd fragment of rabbit immunoglobulin heavy chains

1971 ◽  
Vol 124 (2) ◽  
pp. 301-318 ◽  
Author(s):  
L. E. Mole ◽  
S. A. Jackson ◽  
R. R. Porter ◽  
J. M. Wilkinson
Keyword(s):  
Heavy Chain ◽  
Immunoglobulin G ◽  
Sequence Variation ◽  
Variable Region ◽  
Related Sequence ◽  
Heavy Chains ◽  
Rabbit Immunoglobulin ◽  
Variable Section ◽  
Terminal Section ◽  
Constant Section

The sequence has been completed of the N-terminal 94 residues of the variable section of the Fd fragment of heavy chains from rabbit immunoglobulin G (IgG) of allotype As1. Most of the sequence of the same section from IgG of allotype Aa3 is also reported. These results, in conjunction with a substantial sequence of the variable region of allotype Aa2 reported elsewhere (Fleischman, 1971), show the presence of 16 positions (including six consecutive positions) in which the residue present correlates with the allotype. No allotype-related sequence variation has been found in the constant section of the Fd fragment. This evidence supports the view that two genes code for the heavy chain and it can be used as evidence in favour of somatic mutation as the origin of the variability in the sequence of the N-terminal section. The evolutionary origin of the ‘a’ locus allotypes of rabbit immunoglobulins remains obscure.

Development of diagnostic antibodies against immunoglobulin heavy chain variable region for heavy chain amyloidosis (AH amyloidosis)

2021 ◽  
Vol 71 (4) ◽  
pp. 245-254
Author(s):  
Mayuko Nakagawa ◽  
Masahide Yazaki ◽  
Fuyuki Kametani ◽  
Nagaaki Katoh ◽  
Tsuneaki Yoshinaga ◽  
...  
Keyword(s):  
Heavy Chain ◽  
Variable Region ◽  
Immunoglobulin Heavy Chain ◽  
Heavy Chain Variable Region

scholarly journals The relationship between variable region determinants and antigen specificity on mitogen reactive B cell subsets.

1982 ◽  
Vol 156 (3) ◽  
pp. 924-929 ◽  
Author(s):  
D Primi ◽  
F Mami ◽  
C Le Guern ◽  
P A Cazenave
Keyword(s):  
B Cell ◽  
Variable Region ◽  
Antigen Specificity ◽  
Antigen Binding Site ◽  
V Region ◽  
Set Up ◽  
Cell Mitogen ◽  
B Cell Subsets ◽  
The Relationship ◽  
Beta Galactosidase

On the basis of previous frequency determinations we could set up large numbers of cultures, each containing less than one competent precursor B cell specific for beta-galactosidase or for each of three idiotopes previously found on a monoclonal anti-beta-galactosidase antibody. Cultures were polyclonally activated by either lipopolysaccharide or Nocardia-delipidated cell mitogen. Each culture supernatant was individually tested for hemagglutination activity against sheep erythrocytes coupled with beta-galactosidase or with each of the three purified monoclonal anti-idiotypic antibodies. The results showed that only a minority of those clones positive for only one or two idiotopes recognized antigen. However, all those clones simultaneously positive for the three V region determinants recognized beta-galactosidase. The implications of these results for our understanding of the relationship between the antigen-binding site and idiotope expression are discussed.

scholarly journals Differential reduction of interchain disulphide bonds of mouse immunoglobulin G

1968 ◽  
Vol 107 (6) ◽  
pp. 823-828 ◽  
Author(s):  
Alan R. Williamson ◽  
Brigitte A. Askonas
Keyword(s):  
Heavy Chain ◽  
Immunoglobulin G ◽  
Polyacrylamide Gel Electrophoresis ◽  
Partial Reduction ◽  
Free Light Chains ◽  
Myeloma Protein ◽  
Heavy Chains ◽  
Disulphide Bonds ◽  
Rabbit Immunoglobulin ◽  
Mouse Immunoglobulin

The relative lability of the interchain disulphide bonds of mouse G2a-myeloma protein 5563 was studied as a function of 2-mercaptoethanol concentration. Analysis of partial-reduction mixtures by polyacrylamide-gel electrophoresis and microdensitometry showed that the disulphide bonds between light and heavy chains are much more susceptible to reduction than the bonds between heavy chains. At a low concentration of 2-mercaptoethanol (10mm) the major dissociable products of mouse immunoglobulin G are heavy-chain dimers and free light chains. These findings contrast with the reported behaviour of rabbit immunoglobulin G, for which the lability of inter-heavy-chain bonds was found to exceed that of the bonds linking light and heavy chains (Hong & Nisonoff, 1965); the relative stability of rabbit immunoglobulin G interchain bonds was confirmed in the present study. Examination of human immunoglobulin G and an immunoglobulin G (γ2) of guinea pig showed that at least in the majority of molecules, as with mouse immunoglobulin G, the disulphide bonds between light and heavy chains are more susceptible to reduction than the inter-heavy-chain bonds.

scholarly journals Organization of the variable region of the immunoglobulin heavy-chain gene locus of the rat

2010 ◽  
Vol 62 (7) ◽  
pp. 479-486 ◽  
Author(s):  
Jacobus Hendricks ◽  
Peter Terpstra ◽  
Peter M. Dammers ◽  
Rajesh Somasundaram ◽  
Annie Visser ◽  
...  
Keyword(s):  
Heavy Chain ◽  
Gene Locus ◽  
Variable Region ◽  
Immunoglobulin Heavy Chain ◽  
Chain Gene ◽  
Heavy Chain Gene ◽  
Immunoglobulin Heavy Chain Gene
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