scholarly journals Decreased synthesis of acetylcholine accompanying impaired oxidation of pyruvic acid in rat brain minces

1975 ◽  
Vol 148 (1) ◽  
pp. 17-23 ◽  
Author(s):  
G E Gibson ◽  
R Jope ◽  
J P Blass
Keyword(s):  
Rat Brain ◽  
Pyruvate Dehydrogenase ◽  
Correlation Coefficients ◽  
Competitive Inhibitor ◽  
Co2 Production ◽  
Regression Equations ◽  
Irreversible Inhibitor ◽  
Experimental Conditions ◽  
Acetylcholine Synthesis ◽  
Adult Rat

The relation between pyruvate utilization and acetylcholine synthesis was investigated in minces of adult rat brain. The flux of pyruvate to acetylcholine was less than 1% of that to CO2; nevertheless, a number of agents which inhibited conversion of [1-14C]-pyruvate or [2-14C]pyruvate into 14CO2 were associated with corresponding decreases in the conversion of [2-14C]pyruvate into acetylcholine. The amount of acetylcholine produced by minces of whole rat brain, measured by g.l.c.-mass spectrometry, decreased similarly. Among the inhibitory compounds tested were 3-bromopyruvate, an irreversible inhibitor of pyruvate dehydrogenase; 2-oxobutyrate, a competitive inhibitor of pyruvate dehydrogenase; other 2-oxo acids; and amobarbital and pentobarbital. Linear-regression equations relating CO2 production to acetylcholine synthesis gave correlation coefficients of 0.89-0.93 for the combined observations. The inhibition of acetylcholine synthesis could not be attributed to inhibition of choline acetyltransferase. Incorporation of [2-14C]pyruvate into lipids, proteins and nucleic acids was effected less than that into acetylcholine. Under these experimental conditions, it was shown that pyruvate utilization can limit acetylcholine synthesis.

scholarly journals Effect of phenylpyruvate on pyruvate dehydrogenase activity in rat brain mitochondria

1973 ◽  
Vol 134 (2) ◽  
pp. 539-544 ◽  
Author(s):  
John M. Land ◽  
John B. Clark
Keyword(s):  
Rat Brain ◽  
Dehydrogenase Activity ◽  
Pyruvate Dehydrogenase ◽  
Pyruvate Dehydrogenase Complex ◽  
Competitive Inhibitor ◽  
Brain Mitochondria ◽  
Significant Inhibition ◽  
Complex Activity ◽  
Rat Brain Mitochondria ◽  
Dehydrogenase Complex

1. The effects of phenylpyruvate, a metabolite produced in phenylketonuria, on the pyruvate dehydrogenase-complex activity were investigated in rat brain mitochondria. 2. Pyruvate dehydrogenase activity was measured by two methods, one measuring the release of 14CO2 from [1-14C]pyruvate and the other measuring the acetyl-CoA formed by means of the coupling enzyme, pigeon liver arylamine acetyltransferase (EC 2.3.1.5). In neither case was there significant inhibition of the pyruvate dehydrogenase complex by phenylpyruvate at concentrations below 2mm. 3. However, phenylpyruvate acted as a classical competitive inhibitor of the coupling enzyme arylamine acetyltransferase, with a Ki of 100μm. 4. It was concluded that the inhibition of pyruvate dehydrogenase by phenylpyruvate is unlikely to be a primary enzyme defect in phenylketonuria.

scholarly journals Regional enzyme development in rat brain. Enzymes of energy metabolism

1984 ◽  
Vol 218 (1) ◽  
pp. 139-145 ◽  
Author(s):  
S F Leong ◽  
J B Clark
Keyword(s):  
Rat Brain ◽  
Medulla Oblongata ◽  
Pyruvate Dehydrogenase ◽  
Isocitrate Dehydrogenase ◽  
Citrate Synthase ◽  
Aerobic Glycolysis ◽  
Tricarboxylic Acid Cycle ◽  
Brain Regions ◽  
Glycolytic Enzyme ◽  
Adult Rat

The development of several key enzymes of pyruvate and 3-hydroxybutyrate metabolism and of the tricarboxylic acid cycle was studied in six regions (cerebellum, medulla oblongata and pons, hypothalamus, striatum, mid-brain and cortex) of the neonatal, suckling and adult rat brain (2 days before birth to 60 days after birth). The enzymes whose developmental patterns were studied were: pyruvate dehydrogenase (EC 1.2.4.1), 3-hydroxybutyrate dehydrogenase (EC 1.1.1.30), citrate synthase (EC 4.1.3.7), NAD-linked isocitrate dehydrogenase (EC 1.1.1.41) and fumarase (EC 4.2.1.2). Citrate synthase, isocitrate dehydrogenase and pyruvate dehydrogenase develop as a cluster in each region, although the pyruvate dehydrogenase appears to lag slightly behind the others. As with the glycolytic-enzyme cluster [Leong & Clark (1984) Biochem. J. 218, 131-138] the timing of the development of the activity of this group of enzymes varies from region to region; 50% of the adult activity developed first in the medulla oblongata, followed by the hypothalamus, striatum and mid-brain, and then in the cortex and cerebellum respectively. The 3-hydroxybutyrate dehydrogenase activity also develops earlier in the medulla oblongata than in the other regions. The results are discussed with respect to the neurophylogenetic development of the brain regions studied and the importance of the development of the enzymes of aerobic glycolysis in relationship to the development of neurological maturation.

Expression of neurocan after transient middle cerebral artery occlusion in adult rat brain

2005 ◽  
Vol 25 (1_suppl) ◽  
pp. S217-S217
Author(s):  
Kentaro Deguchi ◽  
Mikiro Takaishi ◽  
Takeshi Hayashi ◽  
Atsuhiko Oohira ◽  
Shoko Nagotani ◽  
...  
Keyword(s):  
Middle Cerebral Artery ◽  
Rat Brain ◽  
Middle Cerebral Artery Occlusion ◽  
Cerebral Artery ◽  
Artery Occlusion ◽  
Adult Rat ◽  
Adult Rat Brain ◽  
Cerebral Artery Occlusion

Heat Power Determination of Dv-290 Refrigerator’s Evaporator on the Basis of Thermodynamic Parameters of Inlet Air / Określenie Mocy Cieplnej Parownika Chłodziarki Dv-290 Na Podstawie Parametrów Termodynamicznych Powietrza Wlotowego

2012 ◽  
Vol 57 (4) ◽  
pp. 911-920
Author(s):  
Bernard Nowak ◽  
Zbigniew Kuczera
Keyword(s):  
Thermodynamic Parameters ◽  
Thermal Power ◽  
Correlation Coefficients ◽  
Specific Humidity ◽  
Physical Parameters ◽  
Air Cooling ◽  
Regression Equations ◽  
Evaporation And Condensation ◽  
Before And After ◽  
The Given

Abstract The present paper introduces a method for calculating the thermal power of DV-290 mining air cooler’s evaporator. The power usually differs from the nominal power given by the manufacturer. The thermodynamic parameters of cooled air are not obtained as a result of in situ measurements, but in indirect manner that is by determining the evaporation and condensation’s pressure values of R407C refrigerant. The pressure dependencies formulated as a function of air enthalpy at the evaporator’s inlet were obtained using calculations of a computer program which solves the system of equations describing heat and mass transfer in the refrigerator’s compressor on the basis of previous measurements of air performed before and after its cooling. The obtained dependencies are demonstrated in a graphical (fig. 2 and fig. 3) and analytical (the regression equations (19) and (20)) manner, the values of correlation coefficients are also presented. For the known evaporation and condensation pressure values of the refrigerant, and thus for its basic physical parameters the complete thermal power of the evaporator was determined, that is its: air cooling overt power, dehumidification occult power, temperature, relative humidity and specific humidity of air after its cooling. In addition, using the mentioned method, the capacity of DV-290 refrigerator’s evaporator is provided for the given thermodynamic parameters of air before cooling, along with air thermodynamic parameters after cooling.

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