Purification and properties of rat brain pyruvate carboxylase

1975 ◽  
Vol 147 (3) ◽  
pp. 385.b1-385.b1
Author(s):  
H. O. Spivey ◽  
W. Flory ◽  
B. D. Peczon ◽  
J. P. Chandler ◽  
R. E. Koeppe
1975 ◽  
Vol 145 (1) ◽  
pp. 25-35 ◽  
Author(s):  
D E Mahan ◽  
I K Mushahwar ◽  
R E Koeppe

Rat brain pyruvate carboxylase was purified 2000-fold and some of its properties and kinetic parameters were investigated. The use of (NH4)2SO4 gradient solubilization on a Celite column and precipitation with polyethylene glycol permitted purification to an estimated 20% purity. Except for a few subtle kinetic differences this enzyme is indistinguishable from rat liver pyruvate carboxylase.


1990 ◽  
Vol 265 (16) ◽  
pp. 9434-9440
Author(s):  
S Y Lee ◽  
S S Sim ◽  
J W Kim ◽  
K H Moon ◽  
J H Kim ◽  
...  

1972 ◽  
Vol 151 (1) ◽  
pp. 48-55 ◽  
Author(s):  
Albert C. Chou ◽  
John E. Wilson

1987 ◽  
Vol 48 (4) ◽  
pp. 1201-1208 ◽  
Author(s):  
Hideo Morino ◽  
Tatsuo Kawamoto ◽  
Masaharu Miyake ◽  
Yasuo Kakimoto

1979 ◽  
Vol 33 (1) ◽  
pp. 53-60 ◽  
Author(s):  
Po Yok Chee ◽  
June L. Dahl ◽  
Leonard A. Fahien

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