scholarly journals Effect of allylisopropylacetamide on Nuclear Ribonucleic Acid synthesis in rat liver

1975 ◽  
Vol 147 (1) ◽  
pp. 185-186 ◽  
Author(s):  
M K Sardana ◽  
M R Satyanarayana Rao ◽  
G Padmanaban
Keyword(s):  
Rat Liver ◽  
Enzyme Induction ◽  
Ribonucleic Acid ◽  
Rna Synthesis ◽  
Acid Synthesis ◽  
Potent Inducer

The porphyrogenic drug allylisopropylacetamide, a potent inducer of delta-aminolaevulinate synthetase, specifically increases nucleoplasmic RNA synthesis in rat liver. The drug-mediated increase in nucleoplasmic RNA synthesis is blocked by cycloheximide and haemin, which also inhibit the enzyme induction.

scholarly journals Ribonucleic acid synthesis in porphyric rat liver induced by 3,5-dicarbethoxy-1,4-dihydrocollidine

1973 ◽  
Vol 136 (1) ◽  
pp. 209-215 ◽  
Author(s):  
Hajime Nawata ◽  
Kenichi Kato
Keyword(s):  
Rat Liver ◽  
Liver Cell ◽  
Ribonucleic Acid ◽  
Rna Synthesis ◽  
Actinomycin D ◽  
Acid Synthesis ◽  
Rapid Decrease ◽  
Aminolaevulinic Acid ◽  
Induction Mechanism

1. Administration of 3,5-dicarbethoxy-1,4-dihydrocollidine to rats caused a marked increase in the activity of δ-aminolaevulinic acid synthetase as well as a slight net increase in RNA in the livers. 2. 3,5-Dicarbethoxy-1,4-dihydrocollidine primarily stimulated the synthesis of RNA in the nucleus of the liver cell. 3. The decrease in RNA synthesis after administration of this drug resulted in a rapid decrease in the activity of this induced enzyme. This was also confirmed by treatment with actinomycin D. 4. From kinetic experiments on synthesis of RNA and δ-aminolaevulinic acid synthetase in vivo the induction mechanism of this enzyme was discussed.

Hormone-induced differentiation of antheridial branches in Achlya ambisexualis: dependence on ribonucleic acid synthesis

1974 ◽  
Vol 20 (7) ◽  
pp. 977-980 ◽  
Author(s):  
David K. Horowitz ◽  
Peter J. Russell
Keyword(s):  
Sexual Differentiation ◽  
Ribonucleic Acid ◽  
Rna Synthesis ◽  
Actinomycin D ◽  
Steroid Hormone ◽  
Acid Synthesis ◽  
Aquatic Fungus ◽  
Induced Differentiation ◽  
Sexual Steroid ◽  
Achlya Ambisexualis

Sexual differentiation in male strains of the aquatic fungus Achlya ambisexualis Raper is induced by antheridiol, a sexual steroid hormone secreted by female strains. Antheridiol-induced initiation of the morphologically distinct antheridial branches in male Achlya is completely prevented when DNA-dependent RNA synthesis is inhibited by actinomycin D. In addition antheridial branch elongation is inhibited to a degree proportional to the concentration of actinomycin D added. Thus, evidence indicates that RNA synthesis is required for antheridiol-induced initiation of antheridial branching and that continued RNA synthesis is required for elongation of antheridial branches.

Inhibition by α-amanitin of chromosomal ribonucleic acid synthesis in rat liver

FEBS Letters ◽  
1972 ◽  
Vol 25 (2) ◽  
pp. 305-308 ◽  
Author(s):  
Giulia Montecuccoli ◽  
F. Novello ◽  
F. Stirpe
Keyword(s):  
Rat Liver ◽  
Ribonucleic Acid ◽  
Acid Synthesis

scholarly journals Decreased ribonucleic acid synthesis in isolated rat liver nuclei during starvation

1970 ◽  
Vol 120 (2) ◽  
pp. 381-384 ◽  
Author(s):  
D. Rickwood ◽  
H. G. Klemperer
Keyword(s):  
Rna Polymerase ◽  
Ammonium Sulphate ◽  
Ribonucleic Acid ◽  
Rna Synthesis ◽  
Actinomycin D ◽  
Acid Synthesis ◽  
Isolated Nuclei ◽  
Isolated Rat Liver ◽  
Liver Nuclei ◽  
Isolated Rat

1. Isolated nuclei from starved rats showed a lowered incorporation of [14C]UMP into RNA. 2. The Mg2+-dependent incorporation was decreased by 30% after 1 day of starvation, but incorporation in the presence of Mn2+ and ammonium sulphate decreased only after longer periods of starvation. 3. RNA synthesis by nuclei in the presence of excess of added RNA polymerase was unchanged after 1 day of starvation and was inhibited by 20% after 4 days. 4. The capacity of nuclei to bind actinomycin D was unchanged after 1 day and was decreased by 20% after 4 days of starvation.

scholarly journals Anabolism versus catabolism of [5-3H]uridine and its relationship to ribonucleic acid labelling in mouse liver after partial hepatectomy

1979 ◽  
Vol 178 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Torsten Yngner ◽  
Claes Engelbrecht ◽  
Lillemor Lewan ◽  
Jan-Erik Annerfeldt
Keyword(s):  
Nucleic Acid ◽  
Partial Hepatectomy ◽  
Ribonucleic Acid ◽  
Mouse Liver ◽  
Rna Synthesis ◽  
Specific Radioactivity ◽  
Acid Synthesis ◽  
Liver Cells ◽  
Control Value

The balance between anabolism and catabolism of [5-3H]uridine was studied in the mouse after partial hepatectomy. Labelling of RNA and UDP-glucose was determined and evaluated in relation to changes in the specific radioactivity of UTP. The amounts of labelled catabolic products of uridine were increased several-fold in liver and blood after partial hepatectomy. The specific radioactivity of RNA decreased to about 60% of the control value at 6h and was in the same range as that of control liver at 24h after operation. Decreased labelling of RNA and UDP-glucose was attributable to decreased specific radioactivity of UTP. No changes in the size of the UTP pool or in the balance between uridine anabolism and catabolism were found that could explain the decreased specific radioactivity of UTP. Rather, the alterations in the labelling of this metabolite induced by the partial hepatectomy may be related to decreased phosphorylating capacity in the liver cells and/or dilution of the labelled precursor in an expanded uridine pool. The enhanced amounts of uridine catabolic products in liver and blood were probably a consequence of accumulation and altered incorporation of the metabolites from the blood into the liver cells. Despite the increased amounts of labelled catabolic products and the decreased labelling of RNA, the results reported here actually suggest decreased uridine catabolism and slightly increased RNA synthesis in mouse liver after partial hepatectomy. The results stress the importance of proper controls in determination of nucleic acid synthesis and in metabolic studies by use of labelled precursors.

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