scholarly journals Thymidine kinase activity in cardiac muscle during embryonic and postnatal development

1974 ◽  
Vol 142 (3) ◽  
pp. 685-690 ◽  
Author(s):  
Paul C. Gillette ◽  
William C. Claycomb
Keyword(s):  
Cardiac Muscle ◽  
Thymidine Kinase ◽  
Postnatal Development ◽  
Kinase Activity ◽  
Sedimentation Coefficient ◽  
Polymerase Activity ◽  
Thymidine Kinase Activity ◽  
Adult Rats ◽  
Ph Optimum ◽  
Km Value

Cytoplasmic thymidine kinase from cardiac muscle of the rat has been characterized. It has a pH optimum of 9.0 and a Km value for thymidine of 1.6μm. The sedimentation coefficient of this enzyme in sucrose gradients is 4.5S, which represents a molecular weight of approx. 69000. Thymidine kinase prepared from cardiac muscle of foetal, neonatal and adult rats is inhibited by dTTP and dTDP; there is neither inhibition nor stimulation by dTMP, dCTP, dATP, dGTP or cyclic AMP. The activity of thymidine kinase in differentiating cardiac muscle of foetal and neonatal rats declines progressively with development, reaching adult values of almost zero by the fifteenth to seventeenth day of postnatal development. This represents a 70-fold decrease in enzyme activity from 3 days before birth to 17 days after birth. The loss of thymidine kinase activity in differentiating cardiac muscle correlates temporally with the cessation of DNA biosynthesis and the loss of cytoplasmic DNA polymerase activity in this tissue.

Thymidine Kinase Activity Is Reduced in the Developing Staggerer Cerebellum

1981 ◽  
Vol 37 (6) ◽  
pp. 1610-1612 ◽  
Author(s):  
Anne Messer ◽  
Michael Savage ◽  
Thomas P. Carter
Keyword(s):  
Thymidine Kinase ◽  
Kinase Activity ◽  
Thymidine Kinase Activity

scholarly journals Increased Serum Thymidine Kinase Activity in Acute Sarcoidosis.

2002 ◽  
Vol 41 (2) ◽  
pp. 129-132 ◽  
Author(s):  
Syunji TAJIMA ◽  
Yoshichika SANDO ◽  
Toshitaka MAENO ◽  
Naoki SAGAWA ◽  
Mami NARA ◽  
...  
Keyword(s):  
Thymidine Kinase ◽  
Kinase Activity ◽  
Thymidine Kinase Activity

Elevated serum thymidine kinase activity in canine splenic hemangiosarcoma*

2011 ◽  
Vol 10 (4) ◽  
pp. 292-302 ◽  
Author(s):  
D. H. Thamm ◽  
D. A. Kamstock ◽  
C. R. Sharp ◽  
S. I. Johnson ◽  
E. Mazzaferro ◽  
...  
Keyword(s):  
Thymidine Kinase ◽  
Kinase Activity ◽  
Elevated Serum ◽  
Thymidine Kinase Activity

Effects of estrogen and progesterone on thymidine kinase activity in the immature rat uterus

1983 ◽  
Vol 145 (6) ◽  
pp. 711-715 ◽  
Author(s):  
Shinobu Sakamoto ◽  
Akio Abe ◽  
Hideki Kudo ◽  
Noriko Yamada ◽  
Keiko Seki ◽  
...  
Keyword(s):  
Thymidine Kinase ◽  
Kinase Activity ◽  
Thymidine Kinase Activity ◽  
Rat Uterus ◽  
Immature Rat

Effects of carcinogenesis on colonic thymidine kinase activity in familial adenomatous polyposis

1992 ◽  
Vol 13 (5) ◽  
pp. 873-876 ◽  
Author(s):  
Shinobu Sakamoto ◽  
Noriyuki Kasahara ◽  
Hideki Kudo ◽  
Takeo Iwama
Keyword(s):  
Familial Adenomatous Polyposis ◽  
Thymidine Kinase ◽  
Kinase Activity ◽  
Adenomatous Polyposis ◽  
Thymidine Kinase Activity

Expression of complete chicken thymidine kinase gene inserted in a retrovirus vector

1984 ◽  
Vol 4 (4) ◽  
pp. 749-754
Author(s):  
P K Bandyopadhyay ◽  
H M Temin
Keyword(s):  
Long Terminal Repeat ◽  
Thymidine Kinase ◽  
Rna Synthesis ◽  
Kinase Activity ◽  
Terminal Repeat ◽  
Thymidine Kinase Activity ◽  
Opposite Orientation ◽  
Kinase Gene ◽  
Intervening Sequences ◽  
High Level

The chicken thymidine kinase (tk) gene was inserted into spleen necrosis virus. Thymidine kinase activity was expressed even when the promoter and terminator sequences for tk RNA synthesis were retained. When the promoter was present in the same orientation as the promoter in the long terminal repeat of the virus, deletions occurred both in the virus and in the tk gene, and the thymidine kinase-transforming activity of the recovered virus was low. Splicing of apparent intervening sequences in the tk gene was also observed. When the orientation of the tk promoter was opposite to the promoter in the long terminal repeat, virus synthesis was diminished, whereas thymidine kinase activity was expressed at an elevated level compared with virus in which the promoter was in the same orientation. However, when the apparent tk promoter was deleted from virus with the tk gene in the opposite orientation, a high level of virus synthesis was observed, probably as a result of absence of interference of RNA synthesis from converging promoters. The intervening sequences in the virus in which the promoters were in opposite orientation were not spliced.

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