scholarly journals Studies on bone matrix glycoproteins. Incorporation of [1-14C]glucosamine and plasma [14C]glycoprotein into rabbit cortical bone

1973 ◽  
Vol 136 (1) ◽  
pp. 125-134 ◽  
Author(s):  
James T. Triffitt ◽  
Maureen Owen
Keyword(s):  
Cortical Bone ◽  
Bone Tissue ◽  
Bone Matrix ◽  
Organic Matrix ◽  
Acidic Components

The radioactively labelled constituents present in bone matrix were compared 12 days after injection of either [14C]glucosamine or plasma [14C]glycoprotein. Both precursors are utilized in the synthesis of organic matrix by bone tissue. Cortical bone from animals injected with [14C]glucosamine contains radioactivity derived from glucosamine and plasma glycoproteins and all glycoprotein fractions are labelled. Plasma [14C]glycoprotein labels the less acidic glycoproteins to a greater extent than the more acidic components. An antibody has been raised against the less-acidic-glycoprotein fraction of bone. The latter contains a glycoprotein of α-mobility that appears to be concentrated specifically in bone tissue and which is present also in plasma. This α-glycoprotein accounts for a large proportion of the components labelled and retained in bone matrix after [14C]glucosamine injection.

scholarly journals The action of vitamin D on the degree of mineralization of bone tissue in rats given adequate amounts of calcium and phosphorus in the diet

1970 ◽  
Vol 24 (1) ◽  
pp. 29-38 ◽  
Author(s):  
P. Rasmussen
Keyword(s):  
Vitamin D ◽  
Vitamin D Deficiency ◽  
Cortical Bone ◽  
Bone Tissue ◽  
Bone Matrix ◽  
Serum Analysis ◽  
Epiphyseal Plate ◽  
Degree Of Mineralization ◽  
Normal Degree ◽  
Calcium And Phosphorus

1. The action of vitamin D on the degree of mineralization of the bone tissue of rats given a diet containing adequate amounts of calcium and phosphorus for 8 weeks has been investigated by quantitative microradiography. The method is described.2. There was a significant reduction in the growth of the vitamin D-deficient rats; the reduction was most marked in the females.3. Serum analysis showed a reduction in Ca, but P was unchanged. However, the Ca × P product was considered to be high enough to permit normal mineralization.4. The degree of mineralization of the cortical bone tissue increased significantly according to the distance from the epiphyseal plate. However, there were no significant differences between vitamin D-treated and vitamin D-deficient animals.5. It is concluded that vitamin D deficiency in rats does not prevent the formation of a bone matrix which can attain a normal degree of mineralization.

Effect of Bone Mineral Content on the Tensile Properties of Cortical Bone: Experiments and Theory

2003 ◽  
Vol 125 (6) ◽  
pp. 785-793 ◽  
Author(s):  
S. P. Kotha ◽  
N. Guzelsu
Keyword(s):  
Mechanical Properties ◽  
Elastic Modulus ◽  
Bone Mineral Content ◽  
Cortical Bone ◽  
Yield Stress ◽  
Bone Tissue ◽  
Bone Mineral ◽  
Mineral Content ◽  
Organic Matrix ◽  
Experimental Values

The effect of mineral volume fraction on the tensile mechanical properties of cortical bone tissue is investigated by theoretical and experimental means. The mineral content of plexiform, bovine bone was lowered by 18% and 29% by immersion in fluoride solutions for 3 days and 12 days, respectively. The elastic modulus, yield strength and ultimate strength of bone tissue decreased, while the ultimate strain increased with a decrease in mineral content. The mechanical behavior of bone tissue was modeled by using a micro-mechanical shear lag theory consisting of overlapped mineral platelets reinforcing the organic matrix. The decrease in yield stress, by the 0.002 offset method, of the fluoride treated bones were matched in the theoretical curves by lowering the shear yield stress of the organic matrix. The failure criterion used was based on failure stresses determined from a failure envelope (Mohr’s circle), which was constructed using experimental data. It was found that the model predictions of elastic modulus got worse with a decrease in mineral content (being 7.9%, 17.2% and 33.0% higher for the control, 3-day and 12-day fluoride-treated bones). As a result, the developed theory could not fully predict the yield strain of bones with lowered mineral content, being 12.9% and 21.7% lower than the experimental values. The predicted ultimate stresses of the bone tissues with lower mineral contents were within ±10% of the experimental values while the ultimate strains were 12.7% and 26.3% lower than the experimental values. Although the model developed in this study did not take into account the presence of hierarchical structures, voids, orientation of collagen molecules and micro cracks, it still indicated that the mechanical properties of the organic matrix depend on bone mineral content.

Collagen-the Ultrastructural Element of the Bone Matrix

2018 ◽  
Vol 69 (7) ◽  
pp. 1706-1709
Author(s):  
Nicoleta Dumitru ◽  
Andra Cocolos ◽  
Andra Caragheorgheopol ◽  
Constantin Dumitrache ◽  
Ovidiu Gabriel Bratu ◽  
...  
Keyword(s):  
Type I Collagen ◽  
Bone Microarchitecture ◽  
Complex Structure ◽  
Bone Matrix ◽  
Organic Matrix ◽  
Bone Diseases ◽  
Bone Collagen ◽  
Type I ◽  
New Therapeutic Approaches ◽  
And Function

There is an increased interest and more studies highlight the fact that bone strength depends not only on bone tissue quantity, but also on its quality, which is characterized by the geometry and shape of bones, trabecular bone microarchitecture, mineral content, organic matrix and bone turnover. Fibrillar type I collagen is the major organic component of bone matrix, providing form and a stable template for mineralization. The biomedical importance of collagen as a biomaterial for medical and cosmetic purposes and the improvement of the molecular, cellular biology and analytical technologies, led to increasing interest in establishing the structure of this protein and in setting of the relationships between sequence, structure, and function. Bone collagen crosslinking chemistry and its molecular packing structure are considered to be distinct features. This unique post-translational modifications provide to the fibrillar collagen matrices properties such as tensile strength and viscoelasticity. Understanding the complex structure of bone type I collagen as well as the dynamic nature of bone tissues will help to manage new therapeutic approaches to bone diseases.

scholarly journals Superior Alignment of Human iPSC-Osteoblasts Associated with Focal Adhesion Formation Stimulated by Oriented Collagen Scaffold

2021 ◽  
Vol 22 (12) ◽  
pp. 6232
Author(s):  
Ryosuke Ozasa ◽  
Aira Matsugaki ◽  
Tadaaki Matsuzaka ◽  
Takuya Ishimoto ◽  
Hui-Suk Yun ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Structural Organization ◽  
Critical Role ◽  
Bone Matrix ◽  
Adhesion Formation ◽  
Focal Adhesions ◽  
Collagen Scaffold ◽  
Patient Specific ◽  
Specific Cell ◽  
Adhesion Behavior

Human-induced pluripotent stem cells (hiPSCs) can be applied in patient-specific cell therapy to regenerate lost tissue or organ function. Anisotropic control of the structural organization in the newly generated bone matrix is pivotal for functional reconstruction during bone tissue regeneration. Recently, we revealed that hiPSC-derived osteoblasts (hiPSC-Obs) exhibit preferential alignment and organize in highly ordered bone matrices along a bone-mimetic collagen scaffold, indicating their critical role in regulating the unidirectional cellular arrangement, as well as the structural organization of regenerated bone tissue. However, it remains unclear how hiPSCs exhibit the cell properties required for oriented tissue construction. The present study aimed to characterize the properties of hiPSCs-Obs and those of their focal adhesions (FAs), which mediate the structural relationship between cells and the matrix. Our in vitro anisotropic cell culture system revealed the superior adhesion behavior of hiPSC-Obs, which exhibited accelerated cell proliferation and better cell alignment along the collagen axis compared to normal human osteoblasts. Notably, the oriented collagen scaffold stimulated FA formation along the scaffold collagen orientation. This is the first report of the superior cell adhesion behavior of hiPSC-Obs associated with the promotion of FA assembly along an anisotropic scaffold. These findings suggest a promising role for hiPSCs in enabling anisotropic bone microstructural regeneration.

scholarly journals Phosphoproteins of chicken bone matrix. Proof of synthesis in bone tissue.

1984 ◽  
Vol 259 (1) ◽  
pp. 290-293
Author(s):  
M J Glimcher ◽  
D Kossiva ◽  
D Brickley-Parsons
Keyword(s):  
Bone Tissue ◽  
Bone Matrix ◽  
Chicken Bone

scholarly journals A novel gelatin/chitooligosaccharide/demineralized bone matrix composite scaffold and periosteum-derived mesenchymal stem cells for bone tissue engineering

2021 ◽  
Vol 25 (1) ◽  
Author(s):  
Thakoon Thitiset ◽  
Siriporn Damrongsakkul ◽  
Supansa Yodmuang ◽  
Wilairat Leeanansaksiri ◽  
Jirun Apinun ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Formation ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Alp Activity

Abstract Background A novel biodegradable scaffold including gelatin (G), chitooligosaccharide (COS), and demineralized bone matrix (DBM) could play a significant part in bone tissue engineering. The present study aimed to investigate the biological characteristics of composite scaffolds in combination of G, COS, and DBM for in vitro cell culture and in vivo animal bioassays. Methods Three-dimensional scaffolds from the mixture of G, COS, and DBM were fabricated into 3 groups, namely, G, GC, and GCD using a lyophilization technique. The scaffolds were cultured with mesenchymal stem cells (MSCs) for 4 weeks to determine biological responses such as cell attachment and cell proliferation, alkaline phosphatase (ALP) activity, calcium deposition, cell morphology, and cell surface elemental composition. For the in vivo bioassay, G, GC, and GCD, acellular scaffolds were implanted subcutaneously in 8-week-old male Wistar rats for 4 weeks and 8 weeks. The explants were assessed for new bone formation using hematoxylin and eosin (H&E) staining and von Kossa staining. Results The MSCs could attach and proliferate on all three groups of scaffolds. Interestingly, the ALP activity of MSCs reached the greatest value on day 7 after cultured on the scaffolds, whereas the calcium assay displayed the highest level of calcium in MSCs on day 28. Furthermore, weight percentages of calcium and phosphorus on the surface of MSCs after cultivation on the GCD scaffolds increased when compared to those on other scaffolds. The scanning electron microscopy images showed that MSCs attached and proliferated on the scaffold surface thoroughly over the cultivation time. Mineral crystal aggregation was evident in GC and greatly in GCD scaffolds. H&E staining illustrated that G, GC, and GCD scaffolds displayed osteoid after 4 weeks of implantation and von Kossa staining confirmed the mineralization at 8 weeks in G, GC, and GCD scaffolds. Conclusion The MSCs cultured in GCD scaffolds revealed greater osteogenic differentiation than those cultured in G and GC scaffolds. Additionally, the G, GC, and GCD scaffolds could promote in vivo ectopic bone formation in rat model. The GCD scaffolds exhibited maximum osteoinductive capability compared with others and may be potentially used for bone regeneration.

Accelerated repair of cortical bone defects using a synthetic extracellular matrix to deliver human demineralized bone matrix

2006 ◽  
Vol 24 (7) ◽  
pp. 1454-1462 ◽  
Author(s):  
Yanchun Liu ◽  
Shama Ahmad ◽  
Xiao Zheng Shu ◽  
R. Kent Sanders ◽  
Sally Anne Kopesec ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Cortical Bone ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Bone Defects ◽  
Demineralized Bone

scholarly journals COMPARATIVE ANALYSIS OF MONOCORTICAL AND BICORTICAL METHODS OF INSTALLING MINI-IMPLANTS

2021 ◽  
pp. 38-40
Author(s):  
O.Yu. Rivis ◽  
V.S. Melnyk ◽  
M.V. Rivis ◽  
K.V. Zombor
Keyword(s):  
Finite Element Method ◽  
Finite Element ◽  
Comparative Analysis ◽  
Cortical Bone ◽  
Bone Tissue ◽  
Orthodontic Treatment ◽  
The Finite Element Method ◽  
Force Load ◽  
Mini Implants ◽  
Element Method

The aim of the study. Carry out a comparative analysis of the support ability of human jaw bone tissue in monocortical and bicortical installation of a mini-implant of own design OMG. Research methods. In order to study biomechanical characteristics of developed OMG mini-implant and bone tissue capacity during monocortical and bicortical installation, the finite element method (MSE) was used. The scheme and finite element 2-D model of bicortical installation of OMG mini-implant (length 8 mm, diameter 1.8 mm) provided full penetration through one layer of cortical bone equal to 1 mm, the entire cancellous bone and immersion in the second layer of cortical bone by 0, 5 mm. No implantation was immersed in the second cortical layer of bone during monocortical installation. A single force load of 1 N was applied in the horizontal direction parallel to the cortical plate of the bone. Results of the study. One of the most important factors leading to the success of the use of a mini-implant is its stability in the process of orthodontic treatment. Quite a high level of failure in the monocortical installation of mini-screws has led to the search for better methods to ensure the stability of their use. This was a bicortical method of fixation, based on the placement of the minig screw in the thickness of the two cortical plates of the jaws. Area for such installation of mini-screws can be a site of a palate and alveolar sprouts at installation of miniimplants through all its thickness. As shown by our data on the use of the finite element method under the force load of the biomechanical system "bone - mini-implant", the stress concentration zone is located in the area of the cortical bone of the jaw. The results of the calculation of the maximum stresses (σmax, MPa) and the maximum possible displacements (umax, mm) of the mini-implant in the biomechanical system "bone - mini-implant" in monocortical installation were, respectively, 8.27 MPa and 0.300 * 10-8 mm and in bicortical installation 6.00 MPa and 0.201 * 10-8 mm. The bicortical method of fixing the mini-implant in the jaw bones significantly increases the ability to resist deformation of this type of biomechanical system under force loads of the mini-implant. In the bicortical method of mini-implant placement, the extreme values of equivalent according to Mises stresses in the upper part of the cortical bone of the jaw are reduced by 27%. This can be explained by a significant increase in the area of contact due to the two layers of the cortical bone of the jaw with the surface of the mini-implant. Conclusion. The bicortical method of installing mini-implants is a more effective and reliable way to provide skeletal support during orthodontic treatment.

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