scholarly journals Nucleotide sequence analyses of the cytoplasmic 5S ribosomal ribonucleic acid from five species of flowering plants

1973 ◽  
Vol 135 (4) ◽  
pp. 845-851 ◽  
Author(s):  
P. I. Payne ◽  
M. J. Corry ◽  
T. A. Dyer
Keyword(s):  
Broad Bean ◽  
Flowering Plants ◽  
5S Rrna ◽  
Sequence Analyses ◽  
Two Dimensional Electrophoresis ◽  
Complementary Sequences ◽  
Ribosomal Ribonucleic Acid ◽  
Rrna Species ◽  
Dimensional Electrophoresis ◽  
Dwarf Bean

Broad-bean 5S rRNA labelled with32P was digested separately with T1 and pancreatic A ribonucleases and the resulting oligonucleotides (20 and 18 respectively) were fractionated by two-dimensional electrophoresis. The oligonucleotides were analysed further and 32 of them have been completely sequenced. They were compared with those of 5S rRNA from dwarf bean, sunflower, tomato and rye. Sequence differences were found at both the 3′- and 5′-termini and at up to nine other positions. Most base substitutions were transitions between C and U. In common with other 5S rRNA species that have been analysed the ends of the molecule in each plant species have complementary sequences.

scholarly journals Nucleotide sequences of chloroplast 5S ribosomal ribonucleic acid in flowering plants

1979 ◽  
Vol 183 (3) ◽  
pp. 595-604 ◽  
Author(s):  
T A Dyer ◽  
C M Bowman
Keyword(s):  
Lemna Minor ◽  
Structural Features ◽  
Flowering Plants ◽  
5S Rrna ◽  
5S Rna ◽  
Homologous Sequences ◽  
Ribosomal Ribonucleic Acid ◽  
Rrna Species ◽  
Strong Resemblance ◽  
Complete Digestion

Evidence for the sequence of duckweed (Lemna minor) chloroplast 5S rRNA was derived from the analysis of partial and complete enzymic digests of the 32P-labelled molecule. The possible sequence of the chloroplast 5S rRNA from three other flowering plants was deduced by complete digestion with T1 ribonuclease and comparison of the sequences of the oligonucleotide products with homologous sequences in the duckweed 5S rRNA. This analysis indicates that the chloroplast 5S rNA species differ appreciably from their cytosol counterparts but bear a strong resemblance to one another and to the 5S rRNA species of prokaryotes. Structural features apparently common to all 5S rRNA molecules are also discussed.

scholarly journals Higher-plant mitochondrial ribosomes contain a 5S ribosomal ribonucleic acid component

1976 ◽  
Vol 157 (1) ◽  
pp. 275-277 ◽  
Author(s):  
C J Leaver ◽  
M A Harmey
Keyword(s):  
Ribonucleic Acid ◽  
Plant Mitochondria ◽  
5S Rrna ◽  
Higher Plant ◽  
Acid Component ◽  
Mitochondrial Ribosomes ◽  
Ribosomal Ribonucleic Acid ◽  
Rrna Species ◽  
Hydrogen Bonded

Ribosomes from higher-plant mitochondria contain 5S rRNA, in contrast with the mitochondrial ribosomes of animals and fungi, in which such a component has not been detected. In common with the ribosomes of prokaryotes and chloroplasts, higher-plant mitochondrial ribosomes do not appear to contain an RNA equivalent to the 5.8 S rRNA that is found in eukaryoytes hydrogen-bonded to the largest of the cytoplasmic rRNA species.

scholarly journals 4.5S ribonucleic acid, a novel ribosome component in the chloroplasts of flowering plants

1979 ◽  
Vol 183 (3) ◽  
pp. 605-613 ◽  
Author(s):  
C M Bowman ◽  
T A Dyer
Keyword(s):  
Ribosomal Rna ◽  
Broad Bean ◽  
Large Subunit ◽  
Flowering Plants ◽  
5S Rrna ◽  
23S Rrna ◽  
Low Molecular Weight ◽  
5.8S Rrna ◽  
Wide Range ◽  
Predominant Variant

A species of low-molecular-weight ribosomal RNA, referred to as ‘4.5S rRNA’, was found in addition to 5S rRNA in the large subunit of chloroplast ribosomes of a wide range of flowering plants. It was shown by sequence analysis that several variants of this RNA may occur in a plant. Furthermore, although in most flowering plants the predominant variant contains about 100 nucleotides, in the broad bean it has less than 80. It seems, therefore, to be much more diverse in size and sequence than the other ribosomal RNA species. Like 5S rRNA, it does not contain modified nucleotides and it is also unusual in having an unphosphorylated 5′-end. It is apparently neither a homologue of cytosol 5.8S rRNA nor a fragment of 23S rRNA.

Sialic Acid Dependent Polypeptide Chain Heterogeneity of Human Fibrinogen Demonstrated by Two-Dimensional Electrophoresis

1982 ◽  
Vol 47 (01) ◽  
pp. 019-021 ◽  
Author(s):  
Cemal Kuyas ◽  
André Haeberli ◽  
P Werner Straub
Keyword(s):  
Sialic Acid ◽  
Polypeptide Chain ◽  
Two Dimensional ◽  
Charge Heterogeneity ◽  
Human Fibrinogen ◽  
Two Dimensional Electrophoresis ◽  
Isoelectric Points ◽  
Polypeptide Chains ◽  
Dimensional Electrophoresis ◽  
Chain Type

SummaryHuman fibrinogen was compared with asialofibrinogen by two-dimensional electrophoresis to evaluate the contribution of sialic acid to the heterogeneity of the γ- and Bβ-polypeptide chains.Reduced fibrinogen showed three major variants for both the γ- and Bβ-chains. In addition two minor γ-bands with a more acidic isoelectric point than the normal γ-chains were observed. Electrophoresis in the second dimension (SDS) suggests that these most acidic bands are γ-chain-variants with a higher molecular weight. In asialofibrinogen only two predominant variants with more alkaline isoelectric points were present in each chain type.It is concluded that enzymatic removal of sialic acid partially reduces the heterogeneity of the γ- and Bβ-polypeptide chains of human fibrinogen, but additional sources producing charge heterogeneity must be sought.

Improvement of Two-dimensional Electrophoresis of Rice (Oryza sativaL.) Proteomics

2012 ◽  
Vol 18 (5) ◽  
pp. 819 ◽  
Author(s):  
Yanhua YANG ◽  
Weitong CUI ◽  
Xiaoyong LIU ◽  
Keming ZHU ◽  
Keping CHEN
Keyword(s):  
Two Dimensional ◽  
Two Dimensional Electrophoresis ◽  
Dimensional Electrophoresis
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