scholarly journals Production and properties of antisera to dexamethasone–protein conjugates

1973 ◽  
Vol 133 (2) ◽  
pp. 401-404 ◽  
Author(s):  
M. C. Dumasia ◽  
D. I. Chapman ◽  
M. S. Moss ◽  
C. O'Connor
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Cross Reaction ◽  
Protein Conjugates ◽  
The Cross

Antibodies to dexamethasone 21-hemisuccinate conjugated to bovine serum albumin were produced in rabbits. Antisera diluted 1:3000 bound 50% of 90pg of [1,2-3H]dexamethasone. The cross-reaction of the antisera with other synthetic and natural corticosteroids was measured.

Effect of some drugs and additives on the cross-linking of bovine serum albumin by glutaraldehyde

1989 ◽  
Vol 57 (3) ◽  
pp. 205-210 ◽  
Author(s):  
A.M. Saleh ◽  
L.K. El-Khordagui ◽  
D.A. Robb ◽  
Florence A.T.
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Cross Linking ◽  
The Cross

Antibodies to Indolealkylamines; Serotonin and Melatonin

1974 ◽  
Vol 52 (3) ◽  
pp. 196-202 ◽  
Author(s):  
Lee J. Grota ◽  
Gregory M. Brown
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Molar Ratio ◽  
Double Immunodiffusion ◽  
Protein Conjugates ◽  
Spectrophotometric Data ◽  
Freund’S Adjuvant ◽  
Freund's Adjuvant

Serotonin, N-acetyl serotonin, and 5-methoxy-N-acetyl serotonin (melatonin) were conjugated to bovine serum albumin (BSA) using formaldehyde. The molar ratio of hapten to protein was determined spectrophotometrically. Spectrophotometric data indicated that serotonin and N-acetyl serotonin but not melatonin were conjugated to bovine serum albumin. Selected hapten–protein conjugates were suspended in Freund's adjuvant and injected into rabbits. Antisera were harvested monthly and screened by double immunodiffusion. Immunodiffusion and inhibition tests indicated that antibodies raised to serotonin–BSA reacted with serotonin and 5-methoxytryptamine but failed to cross react with N-acetyl serotonin or melatonin. Inhibition tests indicated that antibodies to N-acetyl serotonin – BSA reacted with N-acetyl serotonin and cross reacted with melatonin but not with serotonin or 5-methoxytryptamine.

Synthesis and Characterization of Hapten-Protein Conjugates for Antibody Production against Cyanogenic Glycosides

2015 ◽  
Vol 78 (7) ◽  
pp. 1408-1413 ◽  
Author(s):  
ISLAMIYAT FOLASHADE BOLARINWA
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Antibody Titer ◽  
Bovine Serum ◽  
Polyclonal Antibodies ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cyanogenic Glycosides ◽  
Protein Conjugates ◽  
Novel Approach ◽  
Cyanogenic Plants

Consumption of cyanogenic plants can cause serious health problems for humans. The ability to detect and quantify cyanogenic glycosides, capable of generating cyanide, could contribute to prevention of cyanide poisoning from the consumption of improperly processed cyanogenic plants. Hapten-protein conjugates were synthesized with amygdalin and linamarin by using a novel approach. Polyclonal antibodies were generated by immunizing four New Zealand White rabbits with synthesized amygdalin-bovine serum albumin and linamarin-bovine serum albumin immunogen. This is the first time an antibody was produced against linamarin. Antibody titer curves were obtained from all the four rabbits by using a noncompetitive enzyme-linked immunosorbent assay. High antibody titer was obtained at dilutions greater than 1:50,000 from both immunogens. This new method is an important step forward in preventing ingestion of toxic cyanogenic glycosides.

scholarly journals Radioimmunoassay of colchicine

1975 ◽  
Vol 151 (2) ◽  
pp. 413-415 ◽  
Author(s):  
C Boudene ◽  
F Duprey ◽  
C Bohuon
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Biological Fluids ◽  
Cross Reaction ◽  
Colchicine Derivatives

Antibodies to cholchicine were raised in rabbits after injection of N-desacetylthiocolchicine conjugated to bovine serum albumin. Antisera diluted 1:100 bound 50% of 1.6ng of [3H]colchicine. Cross-reaction of the antisera with colchicine derivatives and other drugs was measured and a radioimmunoassay of colchicine in biological fluids was proposed.

Analysis of biomarkers for the cross-linkage of formaldehyde with bovine serum albumin peptides

2008 ◽  
Vol 53 (21) ◽  
pp. 3287-3293 ◽  
Author(s):  
Waqar Ahmad ◽  
YuLin Deng ◽  
Bo Li ◽  
LiLi Li ◽  
Manzoor Ahamd ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Cross Linkage ◽  
The Cross

The Isolation of Antibodies Specific for 5-Methyl-Cytidine-Bovine Serum Albumin

1981 ◽  
Vol 36 (5-6) ◽  
pp. 459-463 ◽  
Author(s):  
Sofia K. Mastronicolis ◽  
Vassilios M. Kapoulas ◽  
Hans Kröger
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Cross Reactivity ◽  
Globulin Fraction ◽  
Bovine Serum Albumine ◽  
The Cross ◽  
Gel Diffusion

Abstract By coupling 5-Methyl-cytidine to bovine serum albumin, a conjugate containing 18 mol of 5-Methyl-cytidine per mol of BSA was prepared. Antibodies to this conjugate were produced by immunization of rabbits. Specificity of the antibody was assesed by gel diffusion in agar containing excessive amounts of the carrier BSA. A slight cross-reactivity with cytidine was eliminated by adsorption on the cross-reacting antigen. The isolation of 7 S immunoglobulin from the total globulin fraction was accomplished by chromatography on DEAE Sephadex-A-50, and a method for the rapid quantitation of the antibodies showed that 12.7% of the IgG protein are monospecific against 5-Methyl-cytidine bovine serum albumine.

Synthesis and Identification of the Ciprofloxacin Hapten-Protein Conjugates

2013 ◽  
Vol 781-784 ◽  
pp. 1244-1247
Author(s):  
Xuan Yun Huang ◽  
Dong Mei Huang ◽  
Yong Fu Shi ◽  
Bing Feng ◽  
Hui Juan Yu ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Sulfonic Acid ◽  
High Sensitivity ◽  
Animal Tissues ◽  
Benzene Sulfonic Acid ◽  
Protein Conjugates ◽  
Optimized Conditions ◽  
Carbodiimide Coupling

In order to generate the specific antibody of small molecular hapten-ciprofloxacin for detecting the Ciprofloxacin residues in edible animal tissues. A modified Carbodiimide coupling method was developed to obtain the immunogenic conjugates through coupling ciprofloxacin with bovine serum albumin in five different proportions. The conjugates were characterized and verified by electrophoresis,the trinitro-benzene-sulfonic acid (TNBS) method and ultraviolet spectra scanning. A quantitative analysis of the density of ciprofloxacin-bovine serum albumin conjugates suggested that a high conjugate density 30:1 could be obtained in the optimized conditions. By immunizing the mice, the antibody showed high sensitivity toward ciprofloxacin with an IC50of 4.69 ng/ml. This study confirmed a practical approach for the ciprofloxacin-bovine serum albumin conjugates, through which the high potent antisera specific for ciprofloxacin hapten can be produced for immunoassay.

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