scholarly journals The purification and properties of extracellular glycosidases of the cellular slime mould Dictyostelium discoideum

1973 ◽  
Vol 133 (1) ◽  
pp. 37-47 ◽  
Author(s):  
D. Every ◽  
J. M. Ashworth
Keyword(s):  
Dictyostelium Discoideum ◽  
Growth Medium ◽  
High Yield ◽  
Kinetic Properties ◽  
Cellular Slime Mould ◽  
Slime Mould ◽  
Physical Chemical ◽  
Purification And Properties ◽  
Cellular Slime

The purification of β-N-acetylhexosaminidase, α-glucosidase, α-mannosidase and β-glucosidase from the spent growth medium of Dictyostelium discoideum strain Ax-2 myxamoebae is described. β-N-Acetylhexosaminidase and α-glucosidase were obtained in high yield and as homogeneous preparations whereas the α-mannosidase preparation consisted of two electrophoretically distinct isoenzymes. The physical, chemical and kinetic properties of these enzymes are described.

scholarly journals Growth of myxamoebae of the cellular slime mould Dictyostelium discoideum in axenic culture

1970 ◽  
Vol 119 (2) ◽  
pp. 171-174 ◽  
Author(s):  
D. J. Watts ◽  
J. M. Ashworth
Keyword(s):  
Cell Differentiation ◽  
Dictyostelium Discoideum ◽  
Axenic Culture ◽  
Axenic Medium ◽  
Cellular Slime Mould ◽  
Slime Mould ◽  
Solid Media ◽  
Cellular Slime

1. A simple axenic medium suitable for the growth of the myxamoebae of a strain of the cellular slime mould Dictyostelium discoideum is described. 2. Procedures suitable for the growth of this strain in liquid and on solid media are described. 3. Conditions suitable for initiating the cell differentiation of myxamoebae grown axenically are described.

Control of cellular differentiation by temperature in the cellular slime mould Dictyostelium discoideum

1984 ◽  
Vol 69 (1) ◽  
pp. 159-165
Author(s):  
M. Maeda
Keyword(s):  
Low Temperature ◽  
Dictyostelium Discoideum ◽  
Cellular Differentiation ◽  
Cellular Slime Mould ◽  
Slime Mould ◽  
Cellular Slime

The effects of low temperature on morphogenesis and cellular differentiation of Dictyostelium discoideum were examined. During incubation at 5 degrees C, the vegetative and preaggregation cells never developed, but cell masses at the aggregation or slug stage developed to form hemispherical, or dumbbell-shaped multicellular structures. By staining with FITC-antispore IgG, the structures formed after 10 days of incubation of tipped aggregates at 5 degrees C were found to be composed of 90% spores, 5% prespore cells and 5% non-stained cells. Since only 20% of the total cells constituting the tipped aggregate had been prespore cells at the beginning of incubation, this showed that spore differentiation proceeded even at low temperature, while stalk differentiation was completely inhibited. Similar results were obtained when the cells were incubated at 3 degrees C. However, at 0 degree C, morphogenesis and cellular differentiation did not occur, although most of the prespore cells at the late culmination stage differentiated incompletely into spores. Possible reasons for the high proportion of spores being induced by low temperature are discussed.

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