scholarly journals Acetoacetate and brain lipogenesis: developmental pattern of acetoacetyl-coenzyme A synthetase in the soluble fraction of rat brain (Short Communication)

1973 ◽  
Vol 132 (3) ◽  
pp. 653-656 ◽  
Author(s):  
Brendan M. Buckley ◽  
Dermot H. Williamson
Keyword(s):  
Rat Brain ◽  
Cholesterol Synthesis ◽  
Short Communication ◽  
Coenzyme A ◽  
Soluble Fraction ◽  
Developing Brain ◽  
Developmental Pattern ◽  
Acetyl Coa ◽  
Brain Cytosol

The existence of acetoacetyl-CoA synthetase in rat brain cytosol is reported. The coupling of this enzyme with cytosolic acetoacetyl-CoA thiolase can provide acetyl-CoA for lipogenesis and cholesterol synthesis without the need for mitochondrial participation. This new route for acetoacetate utilization may be important in developing brain.

scholarly journals Activation of 3-hydroxy-3-methylglutaryl-coenzyme A reductase in homogenates of developing rat brain

1979 ◽  
Vol 182 (2) ◽  
pp. 367-370 ◽  
Author(s):  
W A Maltese ◽  
J J Volpe
Keyword(s):  
Rat Brain ◽  
Cholesterol Synthesis ◽  
Specific Activity ◽  
Postnatal Period ◽  
Developing Brain ◽  
Brain Maturation ◽  
Assay Mixture ◽  
Coa Reductase ◽  
Developing Rat ◽  
Homogenization Medium

The specific activity of 3-hydroxy-3-methylglutaryl-CoA reductase increases when homogenates of developing rat brain are incubated at 37 degrees C or kept on ice. This increase is completely blocked by the addition of F- to the homogenization medium and the assay mixture. The capacity for activation of the reductase is greatest during the early postnatal period and declines as brain maturation proceeds. The data suggest that catalytic modification of the reductase may play a role in the regulation of cholesterol synthesis in the developing brain.

scholarly journals Acetyl-coenzyme A and coenzyme A analogues. Their effects on rat brain choline acetyltransferase

1977 ◽  
Vol 165 (2) ◽  
pp. 321-326 ◽  
Author(s):  
J Rossier
Keyword(s):  
Rat Brain ◽  
Choline Acetyltransferase ◽  
Coenzyme A ◽  
Potent Inhibitor ◽  
Acyl Chain ◽  
Structural Domain ◽  
Acetyl Coa ◽  
Acetyl Coenzyme A ◽  
Weak Inhibitor ◽  
Acetyl Coenzyme

Choline acetyltransferase has the same affinity for acetyl-CoA, propionyl-CoA and butyryl-CoA (Km=1.4 micron). Choline acetyltransferase may use the two latter compounds as substrate, but the longer the acyl chain the lower will be Vmax. CoA is an inhibitor (Ki=1.8 micron). The position of the 3′-phosphate is of primary importance. Desphospho-CoA is a weak inhibitor (Ki=500 micron). 5′-AMP is already an inhibitor (Ki=2500 micron). Phosphopantetheine is not an inhibitor. Dextran Blue is a potent inhibitor (Ki=0.05 micron). Choline acetyltransferase binds to hydrophobic affinity columns. Because of its affinity for nucleotides, affinity for Dextran Blue and hydrophobicity, it is proposed that it contains the ‘nucleotide fold’, which is a common structural domain present in several enzymes binding nucleotides.

Arachidonoyl-Coenzyme A Synthetase and Nonspecific AcylCoenzyme A Synthetase Activities in Purified Rat Brain Microvessels

1987 ◽  
Vol 48 (4) ◽  
pp. 1150-1156 ◽  
Author(s):  
O. Morand ◽  
J. B. Carré ◽  
P Homayoun ◽  
E Niel ◽  
N. Baumann ◽  
...  
Keyword(s):  
Rat Brain ◽  
Coenzyme A ◽  
Brain Microvessels

scholarly journals The association of the sulphogalactosylglycerolipid of rat brain with myelination

1977 ◽  
Vol 166 (3) ◽  
pp. 421-428 ◽  
Author(s):  
Joanne Pieringer ◽  
G. Subba Rao ◽  
Paul Mandel ◽  
Ronald A. Pieringer
Keyword(s):  
Rat Brain ◽  
Adult Life ◽  
Developmental Pattern ◽  
Adult Rats ◽  
Jimpy Mouse ◽  
Myelin Fraction ◽  
Old Rats

The sulphogalactosylglycerolipid of rat brain is closely associated with the process of myelination, as demonstrated by the following observations. 1. The lipid is barely detectable in rat brain before 10 days of age, accumulates rapidly between age 10 and 25 days, and remains relatively constant in amount (between 0.3 and 0.4μmol per brain) thereafter into adult life. 2. The activity of adenosine 3′-phosphate 5′-sulphatophosphate–galactosyldiacylglycerol sulphotransferase is almost absent before 10 days of age, attains a maximum at age 20 days, and slowly decreases thereafter with increasing age. This developmental pattern correlates well with that of other myelin-specific metabolites. 3. Both the concentration of the sulphogalactosylglycerolipid and the activity of sulphotransferase are greatly decreased in the non-myelinating jimpy mouse. 4. The myelin fraction of rat brain contains most of the sulphogalactosylglycerolipid. The lipid occurs in a diacyl and an alkylacyl form. Determinations of the relative amount of each type in brain showed about a 1:1 mixture in both 21-day-old and adult rats. Rats injected with H235SO4 at 20 days of age lost35S from the diacyl form at a higher rate than from the alkylacyl compound over a 21-day period. These data suggest that the diacyl form has a higher turnover than the alkylacyl derivative. The percentage of the total sulpholipid content of brain contributed by the sulphogalactosylglycerolipid is 16% in 21-day-old rats and 8.4% in adult rats.

scholarly journals Catecholamine Binding Macromolecule in Soluble Fraction of Rat Brain

1977 ◽  
Vol 27 (2) ◽  
pp. 213-225 ◽  
Author(s):  
Minoru Inaba ◽  
Kunie Kamata
Keyword(s):  
Rat Brain ◽  
Soluble Fraction

Properties of corticosterone-binding macromolecules from rat brain cytosol

1973 ◽  
Vol 57 (2) ◽  
pp. 387-395 ◽  
Author(s):  
Bernard I. Grosser ◽  
Walter Stevens ◽  
Donal J. Reed
Keyword(s):  
Rat Brain ◽  
Brain Cytosol
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