scholarly journals Stoicheiometry of lactose–proton symport across the plasma membrane of Escherichia coli

1973 ◽  
Vol 132 (3) ◽  
pp. 587-592 ◽  
Author(s):  
Ian C. West ◽  
Peter Mitchell
Keyword(s):  
Escherichia Coli ◽  
Plasma Membrane ◽  
Transport Processes ◽  
Proton Symport

The rate of [14C]lactose inflow into anaerobic, non-metabolizing, Escherichia coli has been correlated with the rate of the simultaneous effective inflow of H+. It is shown that the initial rates of the two transport processes are essentially the same, and it is concluded that the two processes are strictly coupled with a 1:1 stoicheiometry.

Fatty acid uptake in Escherichia coli: regulation by recruitment of fatty acyl-CoA synthetase to the plasma membrane

1993 ◽  
Vol 71 (1-2) ◽  
pp. 51-56 ◽  
Author(s):  
Dev Mangroo ◽  
Gerhard E. Gerber
Keyword(s):  
Escherichia Coli ◽  
Plasma Membrane ◽  
Fatty Acid ◽  
Fatty Acid Uptake ◽  
Fatty Acyl ◽  
Acid Uptake ◽  
Bacterial Membranes ◽  
Rate Limiting Step ◽  
Triton X ◽  
Rate Limiting

Fatty acid uptake in Escherichia coli has been shown to be inhibited by starvation and to be reversed by a short preincubation of the starved cells with D- or L-lactate, succinate, and acetate; these effects on oleate uptake were due to regulation of the rate-limiting step which involves fatty acyl-CoA synthetase. Investigation into the mechanism of regulation of fatty acyl-CoA synthetase showed that D-lactate did not affect the activity of the enzyme directly. Fatty acyl-CoA synthetase was found to be activated by about 20-fold by Triton X-100 and by another 4-fold by the addition of bacterial membranes. D-Lactate treatment was shown to result in coisolation of fatty acyl-CoA synthetase with the plasma membrane; these results are consistent with the interpretation that recruitment of the enzyme to the plasma membrane by D-lactate results in its activation and consequently in the increased level of fatty acid uptake.Key words: fatty acid, uptake, regulation, recruitment, fatty acyl-CoA synthetase, Escherichia coli, plasma membrane.

scholarly journals Measuring the Viscosity of the Escherichia coli Plasma Membrane Using Molecular Rotors

2016 ◽  
Vol 111 (7) ◽  
pp. 1528-1540 ◽  
Author(s):  
Jacek T. Mika ◽  
Alexander J. Thompson ◽  
Michael R. Dent ◽  
Nicholas J. Brooks ◽  
Jan Michiels ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Plasma Membrane ◽  
Molecular Rotors

Identification of Two Arabidopsis thaliana Plasma Membrane Transporters Able to Transport Glucose 1-Phosphate

2019 ◽  
Vol 61 (2) ◽  
pp. 381-392
Author(s):  
Irina Malinova ◽  
Stella Kössler ◽  
Tom Orawetz ◽  
Ulrike Matthes ◽  
Slawomir Orzechowski ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Plasma Membrane ◽  
Carbohydrate Metabolism ◽  
Transport Processes ◽  
Membrane Transporters ◽  
In Planta ◽  
Sugar Derivative ◽  
Sugar Metabolites

Abstract Primary carbohydrate metabolism in plants includes several sugar and sugar-derivative transport processes. Over recent years, evidences have shown that in starch-related transport processes, in addition to glucose 6-phosphate, maltose, glucose and triose-phosphates, glucose 1-phosphate also plays a role and thereby increases the possible fluxes of sugar metabolites in planta. In this study, we report the characterization of two highly similar transporters, At1g34020 and At4g09810, in Arabidopsis thaliana, which allow the import of glucose 1-phosphate through the plasma membrane. Both transporters were expressed in yeast and were biochemically analyzed to reveal an antiport of glucose 1-phosphate/phosphate. Furthermore, we showed that the apoplast of Arabidopsis leaves contained glucose 1-phosphate and that the corresponding mutant of these transporters had higher glucose 1-phosphate amounts in the apoplast and alterations in starch and starch-related metabolism.

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