scholarly journals Isolation and characterization of lipoprotein B from high-density human serum lipoproteins

1972 ◽  
Vol 130 (4) ◽  
pp. 913-917 ◽  
Author(s):  
Gerhard Kostner

1. Lipoprotein B from female Lp(a)-lipoprotein-negative serum was isolated from the fraction of density 1.073–1.125 by using immunoadsorbent; 2.5mg of freeze-dried material was obtained from 100ml of serum from a fasting patient. 2. The hydrated density of this lipoprotein was found to be 1.084g/cm3. A flotation rate F1.200 of 9.4 and lipid/protein ratio 1.40 were found, similar to that of high-density (d 1.073–1.125) lipoprotein preparations. 3. From immunochemical and electrophoretic studies of the intact and totally delipidized lipoprotein B it was concluded that this lipoprotein represents a separate family within the high-density range of human serum lipoproteins. 4. The possibility that the isolated lipoprotein B is an artifact created by the isolation procedure is discussed.

Biochemistry ◽  
1966 ◽  
Vol 5 (12) ◽  
pp. 4044-4053 ◽  
Author(s):  
Petar Alaupovic ◽  
Shafeek S. Sanbar ◽  
Robert H. Furman ◽  
Michael L. Sullivan ◽  
Sandra L. Walraven

Biochemistry ◽  
1969 ◽  
Vol 8 (11) ◽  
pp. 4510-4516 ◽  
Author(s):  
Bernard Shore ◽  
Virgie G. Shore

1981 ◽  
Vol 1 (6) ◽  
pp. 475-485
Author(s):  
J Hirsh ◽  
N Davidson

We have isolated chromosomal deoxyribonucleic acid clones containing the Drosophila dopa decarboxylase gene. We describe an isolation procedure which can be applied to other nonabundantly expressed Drosophila genes. The dopa decarboxylase gene lies within or very near polytene chromosome band 37C1-2. The gene is interrupted by at least one intron, and the primary mode of regulation is pretranslational. At least two additional sequences hybridized by in vivo ribonucleic acid-derived probes are found within a 35-kilobase region surrounding the gene. The developmental profile of ribonucleic acid transcribed from one of these regions differs from that of the dopa decarboxylase transcript.


Biochemistry ◽  
1973 ◽  
Vol 12 (20) ◽  
pp. 3866-3871 ◽  
Author(s):  
H. Nordean Baker ◽  
Richard L. Jackson ◽  
Antonio M. Gotto

Biochimie ◽  
1984 ◽  
Vol 66 (2) ◽  
pp. 159-170 ◽  
Author(s):  
Suzanne Salmon ◽  
Lydie Théron ◽  
Isabel Beucler ◽  
Marise Ayrault-Jarrier ◽  
Jacques Polonovski

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