scholarly journals α1-Acute-phase globulins of rats. Microheterogeneity after isoelectric focusing

1972 ◽  
Vol 130 (1) ◽  
pp. 95-101 ◽  
Author(s):  
A. H. Gordon ◽  
P. J. Dykes
Keyword(s):  
Sialic Acid ◽  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Isoelectric Focusing ◽  
Isoelectric Points ◽  
Individual Bands ◽  
The Stability ◽  
The Individual ◽  
Gel Isoelectric Focusing

1. Improved resolution of mixtures of α1-globulins was obtained by the use of isoelectric focusing. 2. Because material recovered after isoelectric focusing in polyacrylamide gels behaved in a manner which suggested interaction with components derived from the gel, isoelectric focusing when used for preparative purposes was done in a matrix of Sephadex G-75. 3. By this means material from the individual bands formed by isoelectric focusing in 6m-urea could be isolated. The stability of these substances was examined by further isoelectric focusing. 4. Analysis of material that had been shown to be homogenous by isoelectric focusing in the absence of urea and of that from several individual bands derived from the same sample by isoelectric focusing in 6m-urea showed different proportions of sialic acid but no change in amino acid composition. 5. In the presence of 6m-urea the isoelectric points found were increased by 0.14–0.25 pH unit. After removal of most of the sialic acid with neuraminidase the increase was 0.36–0.72 pH unit. After treatment with 0.025m-H2SO4 at 80°C for 1h, which removed all the sialic acid, the increase was 0.40–0.87 pH unit. 6. Because removal of all the sialic acid did not decrease the number of bands formed by isoelectric focusing the observed heterogeneity could not be caused entirely by the presence of various proportions of sialic acid.

Subunit structure of the 12S protein from seeds of Brassica juncea

1975 ◽  
Vol 53 (24) ◽  
pp. 2901-2907 ◽  
Author(s):  
S. L. MacKenzie
Keyword(s):  
Amino Acid ◽  
Amino Acid Composition ◽  
Brassica Juncea ◽  
Acid Composition ◽  
Isoelectric Focusing ◽  
Electrophoretic Analysis ◽  
Subunit Structure ◽  
Isoelectric Points

The 12S globulin from Brassica juncea seeds has been dissociated by 6 M urea: 0.1 M mercaptoethanol and the resultant products have been partially separated by preparative isoelectric focusing. Species that have the isoelectric points 4.75, 5.15, 5.40, 6.25, 6.70, 7.00, 7.20, 7.70, 8.30, 8.90, and 9.15 were detected. The results of poly aery lamide-gel electrophoretic analysis and the amino acid composition of the fractions suggested that the isoelectric species were distinct subunits.

scholarly journals Studies on Ovalbumin V. The Amino Acid Composition and Some Properties of Chicken, Duck, and Turkey Ovalbumins

1970 ◽  
Vol 23 (5) ◽  
pp. 1221 ◽  
Author(s):  
MB SmIth ◽  
Joan F Back
Keyword(s):  
Amino Acid ◽  
Amino Acid Composition ◽  
Ammonium Sulphate ◽  
Acid Composition ◽  
Isoelectric Focusing ◽  
Ph Gradient ◽  
Ammonium Sulphate Fractionation

The ovalbumins from chicken, duck, and turkey eggs were prepared by ammonium sulphate fractionation and purified by isoelectric focusing in a pH gradient from 3 to 6. Amino acid analyses show a closer relationship between turkey and chicken ovalbumins than between duck and chicken ovalbumins.

ORGANIC NITROGEN DISTRIBUTION IN SELECTED PEATS AND PEAT FRACTIONS

1978 ◽  
Vol 58 (2) ◽  
pp. 237-249 ◽  
Author(s):  
F. J. SOWDEN ◽  
H. MORITA ◽  
M. LEVESQUE
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Acid Amide ◽  
Amino Sugar ◽  
The Other ◽  
Total N ◽  
Mesh Material ◽  
The Individual

The nitrogenous products formed by 6 N HCl hydrolysis of a variety of peats including a cultivated mesic profile, a virgin humic profile, a sedge, a woody and a sphagnum fibric peat were studied. Peat fractions separated according to particle size, woody and herbaceous materials isolated from a peat sample, and Typha and Carex plants growing on peat soils were also analyzed. Of the two profiles examined, the cultivated mesic peat showed significant quantitative changes in the content of individual amino acids throughout the profile. Also the amino sugar and amino acid N increased to a maximum, then decreased. On the other hand, the virgin humic peat profile exhibited random variations in the content of the individual amino acids and in the amount of amino acid N. Of the other peats examined, the fibric sphagnum had the highest percentage of amino acid N. Among the separates, the 100- to 200-mesh material had the highest proportion of amino acid N. The amino acid composition of the plant remains from the peat was similar to that of the peat from which it was isolated. The amino acid composition of the Typha and Carex plants was different from that of peat in that 75% of their total N content could be accounted for on the basis of amino acid, amide and amino sugar N. The amounts of hydroxyproline and the amino sugars in peats vary more than that of the amino compounds. In contrast to inorganic soils, which tend to have a similar amino acid composition, these limited data suggest that the amino acid and amino sugar contents of peats may be characteristic of individual bogs.

scholarly journals Biosynthesis of hemoglobin F Malta-I in culture by adult circulating erythropoietic precursors

Blood ◽  
1980 ◽  
Vol 55 (2) ◽  
pp. 334-337
Author(s):  
K Kidoguchi ◽  
M Ogawa ◽  
JD Karam ◽  
RG Schneider ◽  
U Carpentieri
Keyword(s):  
Amino Acid ◽  
Normal Distribution ◽  
Frequency Distribution ◽  
Isoelectric Focusing ◽  
Peripheral Blood ◽  
Significant Variation ◽  
Hemoglobin F ◽  
The Individual ◽  
Gel Isoelectric Focusing ◽  
Clonal Assay

By using a methylcellulose clonal assay, we cultured peripheral blood erythropoietic precursors (BFU-E) from an adult couple whose child had HbF Malta-I(gamma 117 His leads to Arg), a G gamma variant, and measured the synthetic rates of HbA, HbF, and HbF Malta-I. Hemoglobin was labeled with 14C-amino acid in culture, separated by slab gel isoelectric focusing technique, and quantitated by autoradiographic or fluorographic method. Culture of BFU-E from both parents revealed significant HbF biosynthesis. HbF Malta-I was present in culture of the father's cells and comprised about 24% of total HbF. When we analyzed Hb biosynthesis in individual bursts, all bursts contained HbA and HbF in varying ratios. The frequency distribution of the individual bursts differing in percentages of HbF biosynthesis approached normal distribution. While the relative ratio of HbF Malta-I to total HbF biosynthesis in individual bursts also revealed significant variation, its frequency distribution did not show a normal distribution. There was a positive correlation between the ratios of HbF/Hb and HbF Malta- I/HbF in individual bursts.

Isoelectric focusing electrophoresis of protein-ligand conjugates: effect of the degree of substitution

1991 ◽  
Vol 37 (1) ◽  
pp. 87-90 ◽  
Author(s):  
Mlnas S Barbarakis ◽  
Leonldas G Bachas
Keyword(s):  
Amino Acid ◽  
Theoretical Model ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Isoelectric Focusing ◽  
Isoelectric Point ◽  
Calibration Curve ◽  
Degree Of Substitution

Abstract The degree of substitution of protein-ligand conjugates can be determined from the change of the isoelectric point (pI) of the protein when ligand molecules are attached to its surface. Specifically, the pI values of conjugates with known degrees of substitution are obtained by isoelectric focusing electrophoresis and are used to generate a calibration curve that relates these two variables. The shape of the curve is sigmoidal and can be predicted by a theoretical model that takes into account the degree of substitution and the amino acid composition of the protein. By using such a calibration curve, one may estimate the degree of substitution of a given protein-ligand conjugate from its pI value. The applicability of the method is demonstrated with conjugates of pyridoxal 5-phosphate and avidin.

AMINO ACID COMPOSITION OF THE PROTEINS OF THE MUSCLES AND ORGANS OF THE NORMAL, CASTRATED AND TESTOSTERONE TREATED GUINEA PIG

1964 ◽  
Vol 45 (4) ◽  
pp. 613-622 ◽  
Author(s):  
Charles D. Kochakian ◽  
John Hill ◽  
Giovanni Costa
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Seminal Fluid ◽  
Normal Growth ◽  
Exchange Chromatography ◽  
Seminal Vesicles ◽  
Androgen Treatment ◽  
The Individual

ABSTRACT The protein of several muscles, liver, kidney, prostate, seminal vesicles and seminal vesicle fluid of normal, castrated and testosterone propionate or testosterone treated guinea pigs was prepared by either trichloracetic or perchloric acid treatment. The extracted and defatted proteins were hydrolysed with acid and the amino acid composition determined by ion exchange chromatography. Each tissue contained all of the commonly occurring amino acids. The seminal fluid also contained several other unidentified ninhydrin reacting compounds. The quantity of each acid changed in direct proportion with the changes in weight of the tissues produced by castration or androgen treatment. The nine different muscles analysed ranged in no dependency to very great dependency on androgen for normal growth but the amino acid composition of their proteins was practically identical. The quantity of the individual amino acids of the fluid of the seminal vesicles was very different from that of the organ.

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