scholarly journals The effect of anoxia on cerebral acid hydrolases in the five-day-old rat

1972 ◽  
Vol 129 (5) ◽  
pp. 1079-1084 ◽  
Author(s):  
B. P. F. Adlard ◽  
S. W. De Souza
Keyword(s):  
Acid Phosphatase ◽  
High Speed ◽  
Recovery Period ◽  
Brain Homogenate ◽  
Acid Hydrolases ◽  
Whole Brain ◽  
Immature Rat ◽  
Glucuronidase Activity ◽  
Anaesthetized Rats ◽  
Triton X

1. Five-day-old anaesthetized rats subjected to slow, prolonged asphyxia (50–55 min) were either allowed to die or resuscitated when at the point of death. Activities of various cerebral acid hydrolases known to be associated with lysosomes were determined in these animals and in littermate controls. 2. Asphyxia to death resulted in a significant increase in the activities of acid phosphatase, cathepsin (pH5.0) and β-glucuronidase in whole-brain homogenates. 3. The effect of asphyxia on β-glucuronidase activity was not apparent when the assay was performed in the presence of Triton X-100 (0.1%, v/v). 4. In resuscitated animals whole-brain-homogenate β-glucuronidase activity showed the greatest increase (31%) 15 min after recovery. After a 60 min recovery period differences between control and asphyxiated animals were no longer apparent. 5. In animals anoxiated to death activities of acid phosphatase and β-N-acetylglucosaminidase in brain high-speed supernatants were significantly higher than in controls. Acid phosphatase activity was similarly increased in asphyxiated animals resuscitated for 5 or 60 min. 6. It is suggested that the response of the immature rat brain to asphyxia involves a disruption or increased fragility of lysosomal particles.

scholarly journals Studies on the structure-bound sedimentability of some rat liver lysosome hydrolases

1971 ◽  
Vol 122 (3) ◽  
pp. 363-371 ◽  
Author(s):  
F. M. Baccino ◽  
G. A. Rita ◽  
Maria Franca Zuretti
Keyword(s):  
Acid Phosphatase ◽  
Rat Liver ◽  
High Speed ◽  
Particulate Material ◽  
The Other ◽  
Acid Proteinase ◽  
Freezing And Thawing ◽  
Clear Cut ◽  
Almost All ◽  
Triton X

1. Lysosome-rich fractions from rat liver were subjected to several disruptive procedures: osmotic lysis or freezing and thawing in different media, shearing forces in a high-speed blender, treatment with Triton X-100. 2. The soluble and particulate phases were then separated by high-speed centrifugation and assayed for their content of acid phosphatase, β-galactosidase, β-N-acetylglucosaminidase, acid proteinase, acid ribonuclease, acid deoxyribonuclease and protein. 3. The degree of elution of these hydrolases appeared to depend on both the enzyme species and the treatment. The resulting patterns of solubilization were rather complex, so that a clear-cut discrimination between soluble and structure-bound enzymes could not always be traced. 4. Although only β-galactosidase was readily solubilizable after all treatments, acid proteinase could also be extensively eluted from the sedimentable material in the presence of EDTA and acid phosphatase was fully extracted by Triton X-100. On the other hand, considerable proportions of the other activities could not be solubilized by any of the procedures used. 5. In other experiments, the adsorbability of hydrolases on subcellular structures was investigated by measuring the partition between sedimentable particles and soluble fraction of solubilized enzymes added to ‘intact’ liver homogenates. 6. Large proportions of acid proteinase, ribonuclease and deoxyribonuclease, and almost all of β-N-acetylglucosaminidase, were found to be adsorbed on the particulate material.

Binding of Human Platelet Glycoprotein lb and Actin to Fragments of Actin-Binding Protein

1992 ◽  
Vol 67 (02) ◽  
pp. 252-257 ◽  
Author(s):  
Anne M Aakhus ◽  
J Michael Wilkinson ◽  
Nils Olav Solum
Keyword(s):  
Actin Filaments ◽  
High Speed ◽  
Binding Protein ◽  
Protein A ◽  
Actin Binding ◽  
Platelet Glycoprotein ◽  
Actin Binding Protein ◽  
Crossed Immunoelectrophoresis ◽  
Triton X ◽  
Calpain Inhibitors

SummaryActin-binding protein (ABP) is degraded into fragments of 190 and 90 kDa by calpain. A monoclonal antibody (MAb TI10) against the 90 kDa fragment of ABP coprecipitated with the glycoprotein lb (GP lb) peak observed on crossed immunoelectrophoresis of Triton X-100 extracts of platelets prepared without calpain inhibitors. MAb PM6/317 against the 190 kDa fragment was not coprecipitated with the GP lb peak under such conditions. The 90 kDa fragment was adsorbed on protein A agarose from extracts that had been preincubated with antibodies to GP lb. This supports the idea that the GP Ib-ABP interaction resides in the 90 kDa region of ABP. GP lb was sedimented with the Triton-insoluble actin filaments in trace amounts only, and only after high speed centrifugation (100,000 × g, 3 h). Both the 190 kDa and the 90 kDa fragments of ABP were sedimented with the Triton-insoluble actin filaments.

scholarly journals Production of Porous Ceramic Materials from Spent Fluorescent Lamps

2021 ◽  
Vol 11 (13) ◽  
pp. 6056
Author(s):  
Egle Rosson ◽  
Acacio Rincón Rincón Romero ◽  
Denis Badocco ◽  
Federico Zorzi ◽  
Paolo Sgarbossa ◽  
...  
Keyword(s):  
Rare Earth ◽  
Rare Earth Elements ◽  
High Speed ◽  
Porous Ceramic ◽  
Ceramic Materials ◽  
Porous Ceramics ◽  
Fluorescent Lamps ◽  
Naoh Solution ◽  
Commercial Glass ◽  
Triton X

Spent fluorescent lamps (SFL) are classified as hazardous materials in the European Waste Catalogue, which includes residues from various hi-tech devices. The most common end-of-life treatment of SFL consists in the recovery of rare earth elements from the phosphor powders, with associated problems in the management of the glass residues, which are usually landfilled. This study involves the manufacturing of porous ceramics from both the coarse glass-rich fraction and the phosphor-enriched fraction of spent fluorescent lamps. These porous materials, realizing the immobilization of Rare Earth Elements (REEs) within a glass matrix, are suggested for application in buildings as thermal and acoustic insulators. The proposed process is characterized by: (i) alkaline activation (2.5 M or 1 M NaOH aqueous solution); (ii) pre-curing at 75 °C; (iii) the addition of a surfactant (Triton X-100) for foaming at high-speed stirring; (iv) curing at 45 °C; (v) viscous flow sintering at 700 °C. All the final porous ceramics present a limited metal leaching and, in particular, the coarse glass fraction activated with 2.5 M NaOH solution leads to materials comparable to commercial glass foams in terms of mechanical properties.

scholarly journals Lysosomal enzymes and vitamin E deficiency

1967 ◽  
Vol 21 (1) ◽  
pp. 127-136 ◽  
Author(s):  
J. Bunyan ◽  
J. Green ◽  
A. T. Diplock ◽  
D. Robinson
Keyword(s):  
Acid Phosphatase ◽  
Subcellular Distribution ◽  
Subcutaneous Tissue ◽  
Hydrolytic Activity ◽  
Breast Muscle ◽  
Lysosomal Hydrolases ◽  
Exudative Diathesis ◽  
Glucuronidase Activity ◽  
Deficiency Diseases ◽  
Increased Activity

1. The activities of several lysosomal hydrolases were measured in the tissues of chicks suffering from nutritional muscular dystrophy, encephalomalacia or exudative diathesis.2. In dystrophic breast muscle, β-glucuronidase was raised five- to six-fold, cathepsin fourfold and acid phosphatase 1.5-fold. No change was found in the subcellular distribution of β-glucuronidase.3. Chicks with encephalomalacia showed no changes in the β-glucuronidase, β-galactosidase, acid phosphatase or β-acetylglucosaminase activities of cerebellum or brain. Subcellular distribution of β-glucuronidase and β-galactosidase in these tissues was also unchanged.4. In exudative diathesis, hydrolases were found in the exudate, and there was increased activity in the subcutaneous tissue first showing haemorrhages. Increased hydrolytic activity was found in liver, spleen and kidney. Breast muscle was not always affected by the exudative condition, but, when it too degenerated, its hydrolase activity increased.5. β-Glucuronidase activity was measured in the serums of chicks suffering from each of the three deficiency diseases. None of the diseases caused a rise in activity.

scholarly journals Content and thrombin-induced release of acid hydrolases in gel-filtered platelets from patients with storage pool disease

Blood ◽  
1975 ◽  
Vol 46 (1) ◽  
pp. 131-142 ◽  
Author(s):  
H Holmsen ◽  
CA Setkowsky ◽  
B Lages ◽  
HJ Day ◽  
HJ Weiss ◽  
...  
Keyword(s):  
Acid Phosphatase ◽  
Normal Subjects ◽  
Acid Hydrolases ◽  
Release Reaction ◽  
Dense Granules ◽  
Storage Pool ◽  
Low Concentrations ◽  
Storage Pool Disease ◽  
Beta Galactosidase

Abstract The levels of four acid hydrolases, beta-N-acetyl glucosaminidase, beta- glucuronidase, beta-galactosidase, and acid phosphatase, and the extent of their release (release II) by thrombin was determined in platelets from nine normal subjects, nine patients with storage pool disease, and in normal platelets which had been exposed to aspirin. The levels of all four hydrolases were normal in patients with SPD. However, release of three of these hydrolases (acid phosphatase was an exception) by low concentrations of thrombin (0.015 and 0.04 U/ml) was decreased in the patients as a group, although considerable variation in the extent of release of each enzyme was noted. In contrast, aspirin failed to inhibit release II in normal platelets (except for a slight impairment in the release of beta-N-acetyl glucosaminidase), although release I (serotonin, ATP and ADP) was inhibited. All release defects could be overcome by using higher concentrations of thrombin (0.2 U/ml). The normal levels of acid hydrolases in the platelets of patients with SPD (who are deficient in the platelet dense granules) suggest that these enzymes are not normally stored in the dense granules, but rather in alpha-granules. The findings also support the conclusions of previous studies that the release reaction is impaired in SPD. This release defect appears to be different from that seen in normal platelets after exposure to aspirin.

scholarly journals High Speed Roll-to-Roll Printable Transistor Enabled by a Pulsed Light Curable CNT Ink

2019 ◽  
Vol 3 (2) ◽  
pp. 33
Author(s):  
Peter Mack Grubb ◽  
Farzad Mokhtari Koushyar ◽  
Travis Lenz ◽  
Aref Asghari ◽  
Gongwen Gan ◽  
...  
Keyword(s):  
High Speed ◽  
Flash Lamp ◽  
Pulsed Light ◽  
Production Methods ◽  
On Demand ◽  
Roll To Roll ◽  
Drop On Demand ◽  
Visible Light Range ◽  
Strong Energy ◽  
Triton X

This paper reports the first high speed roll-to-roll printable transistor using a carbon nanotube (CNT) semiconducting layer. The transistor is made possible through the development of a pulsed light curable CNT ink compatible with typical drop on demand inkjet cartridges. This CNT ink uses a xylene based solvent with methanol, glycerin, and Triton X-100 modifiers to create an evaporable solution with appropriate absorption spectra for a mercury or xenon flash lamp with strong energy transmission in the UVB to mid visible light range, allowing the solution to absorb the energy from the flash lamp and evaporate. Transistor dimensions were defined by the capabilities of a typical roll-to-roll drop on demand cartridge. The final device demonstrated an on/off ratio of 104, representing performance similar to gravure printed devices. This represents the first CNT ink which can be used in high speed production methods without long thermal curing steps in the workflow.

Experimental Investigation of Low Weber Number Post-Impact Drop Spreading on Solid Substrates

2010 ◽  
Author(s):  
Milind A. Jog ◽  
Raj M. Manglik
Keyword(s):  
Surface Tension ◽  
High Speed ◽  
Pure Water ◽  
Surfactant Solution ◽  
Video Camera ◽  
Dynamic Surface Tension ◽  
Temporal Variations ◽  
Dynamic Surface ◽  
Post Impact ◽  
Triton X

The post-impact spreading and recoil behaviors of droplets of pure liquids (water and ethanol) and aqueous solution of Triton X-100 (a surfactant) on a dry horizontal hydrophilic (glass) substrate are investigated for low Weber numbers. The evolution of drop shape during spreading and recoil are captured using a high-speed (4,000 frames per second) digital video camera. Digital image-processing was used to determine the spread and height of the liquid film on the surface from each frame. Unlike pure liquids, the liquid-gas interfacial tension for surfactant solution is a function of surface age, where surface tension is that of the solvent at zero time and then reaches an equilibrium value with increasing surface age. Furthermore, the equilibrium surface tension is a function of the surfactant concentration, which decreases from that of the solvent at zero concentration to that at the critical micelle concentration (CMC), and remains essentially constant thereafter. The surface tension of aqueous Triton X-100 solution varies from that of pure water to nearly that of ethanol. As such the comparison of transient droplet-impact-spreading-recoil behavior of the three liquids, or their temporal variations of the spread and the flattening factor, provides a basis for understanding the role of dynamic surface tension and surface wettability.

scholarly journals BETA GLUCURONIDASE-RICH CYTOPLASMIC PARTICLES IN ANDROGEN-STIMULATED MOUSE KIDNEY

1963 ◽  
Vol 16 (2) ◽  
pp. 253-258 ◽  
Author(s):  
Andrew G. Plaut ◽  
William H. Fishman
Keyword(s):  
Alkaline Phosphatase ◽  
Acid Phosphatase ◽  
Specific Activity ◽  
Mouse Kidney ◽  
The Other ◽  
Mouse Testis ◽  
Glucuronidase Activity ◽  
Other Hand ◽  
Fraction I ◽  
Gonadotropic Hormones

Androgens produced by stimulating mouse testis with gonadotropic hormones cause a rise in renal ß-glucuronidase but not an increase in acid or alkaline phosphatase. All subcellular components increase in ß-glucuronidase activity, with a relatively greater increment in particulate enzyme as compared with that free in the cytoplasm (non-sedimentable). A small percentage of recovered ß-glucuronidase, acid phosphatase, and alkaline phosphatase is found in material which rises to the surface during centrifugation in sucrose media (fraction I). The specific activity of ß-glucuronidase and acid phosphatase in this fraction is normally quite high with respect to the homogenate, while that of alkaline phosphatase is not. On the other hand, the fraction I material from androgen-stimulated mice exhibits a further increase in specific activity with respect to ß-glucuronidase and not acid phosphatase. It thus appears that there is an independence in the behavior of individual enzymes in response to physiologic stimuli in spite of obvious morphologic proximity.

Cytoplasmic Membrane Lipoprotein LppC ofStreptococcus equisimilis Functions as an Acid Phosphatase

1998 ◽  
Vol 64 (7) ◽  
pp. 2439-2442 ◽  
Author(s):  
Horst Malke
Keyword(s):  
Acid Phosphatase ◽  
Cell Membrane ◽  
Cytoplasmic Membrane ◽  
Structural Homology ◽  
Whole Cells ◽  
Specific Enzymatic Activity ◽  
Acidic Conditions ◽  
Membrane Lipoprotein ◽  
Triton X ◽  
Membrane Lipoproteins

ABSTRACT The function of the streptococcal cytoplasmic membrane lipoprotein, LppC, was identified with isogenic Streptococcus equisimilis H46A and Escherichia coli JM109 strain pairs differing in whether they contained [H46A and JM109(pLPP2)] or lacked (H46A lppC::pLPP10 and JM109) the functional lppC gene for comparative phosphatase determinations under acidic conditions. lppC-directed acid phosphatase activity was demonstrated zymographically and by specific enzymatic activity assays, with whole cells or cell membrane preparations as enzyme sources. LppC acid phosphatase showed optimum activity at pH 5, and the enzyme activity was unaffected by Triton X-100, l-(+)-tartaric acid, or EDTA. Database searches revealed significant structural homology of LppC to the Streptococcus pyogenes LppA,Flavobacterium meningosepticum OplA, Helicobacter pylori HP1285, and Haemophilus influenzae Hel [e (P4)] proteins. These results suggest a possible function for these proteins and establish a novel function of streptococcal cell membrane lipoproteins.

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