scholarly journals Nucleotide sequences of two fragments from the coat-protein cistron of bacteriophage R17 ribonucleic acid

1972 ◽  
Vol 128 (5) ◽  
pp. 993-1006 ◽  
Author(s):  
P. G. N. Jeppesen ◽  
B. G. Barrell ◽  
F. Sanger ◽  
A. R. Coulson
Keyword(s):  
Amino Acids ◽  
Coat Protein ◽  
Ribonucleic Acid ◽  
Nucleotide Sequences ◽  
Polyacrylamide Gel ◽  
Base Pairing ◽  
Ribonuclease T1 ◽  
Partial Digestion

Bacteriophage R17 RNA was labelled with 32P and was subjected to partial digestion with ribonuclease T1. The products were fractionated by ionophoresis on polyacrylamide gel. Two fragments were purified and their nucleotide sequences determined by methods involving complete and further partial digestion with ribonucleases A and T1. Fragment 20 had a sequence that coded for the amino acids in positions 32–53 of the coat protein of the bacteriophage. Fragment 20X, on further purification in 7m-urea, gave rise to two smaller nucleotides whose sequences coded for the amino acids in positions 56–66 and 67–76 of the coat protein. The sequence of the two fragments was such that they could be written in the form of loops stabilized by base-pairing.

scholarly journals The nucleotide sequences of some large ribonuclease T1 products from bacteriophage R17 ribonucleic acid

1971 ◽  
Vol 124 (2) ◽  
pp. 357-366 ◽  
Author(s):  
Peter G. N. Jeppesen
Keyword(s):  
Escherichia Coli ◽  
Thin Layer ◽  
Ribonucleic Acid ◽  
Chromatographic Separation ◽  
Nucleotide Sequences ◽  
Two Dimensional ◽  
Ribonuclease T1 ◽  
Unique Pattern ◽  
Fingerprinting Technique

A method of ‘fingerprinting’ high-molecular-weight32P-labelled RNA species, using a two-dimensional thin-layer-chromatographic separation of ribonuclease T1 digestion products, has been applied to RNA from the Escherichia coli bacteriophage R17. The ‘fingerprinting’ technique, besides giving a unique pattern that can be used as a characterization of the RNA, has made it possible to isolate a number of the larger oligonucleotides and to determine their nucleotide sequences.

scholarly journals Potato Tuber Necrotic Ringspot Disease Occurring in Japan: Its Association with Potato virus Y Necrotic Strain

Plant Disease ◽  
2000 ◽  
Vol 84 (10) ◽  
pp. 1109-1115 ◽  
Author(s):  
Kazusato Ohshima ◽  
Kazuya Sako ◽  
Chikako Hiraishi ◽  
Akio Nakagawa ◽  
Kazutoshi Matsuo ◽  
...  
Keyword(s):  
Amino Acids ◽  
Coat Protein ◽  
Potato Tuber ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Nucleotide Sequences ◽  
Potato Tubers ◽  
Necrotic Spots ◽  
Nagasaki Prefecture ◽  
Necrotic Symptoms

Unfamiliar necrotic symptoms on or within potato tubers of cultivars Nishiyutaka and Dejima were observed in Nagasaki prefecture, Japan, in 1992. Symptoms were typically on the surface of the tuber, which either protruded at first and then became sunken, or showed necrotic spots, with necrosis within the tubers. Symptoms sometimes appeared at harvesting but more often appeared after storage for several months. Investigations revealed that the causal agents of the disease were isolates of Potato virus Y necrotic strain (PVYNTN) and the disease was potato tuber necrotic ringspot disease (PTNRD), previously reported in Europe and Lebanon. Five potato cultivars were inoculated with an isolate from necrotic tubers. The highest percentage of progeny tubers showing PTNRD was found in cv. Nishiyutaka (23.3%). In contrast, cvs. Shima-bara, Mayqueen, and Danshaku showed a low percentage of PTNRD. Additional potato tubers with PTNRD were also observed after storage of the tubers. To investigate the relatedness between isolates of PVYNTN and of necrotic strain PVYN, previously isolated in Japan, Nishiyu-taka was inoculated with an isolate of PVYN, which also induced PTNRD. Nucleotide sequences of coat protein (CP) genes of six PVYNTN isolates were determined. The CPs were 267 amino acids in length, with a substitution of one or no amino acid among each of the six isolates. The phylogenetic relationship based on nucleotide sequences of CP genes showed that these six PVYNTN isolates clustered together with PVYN isolates. This is the first report of PTNRD caused by PVYNTN isolates in Asia.

scholarly journals Characterization of the Major Compounds Found in Ribonuclease T1 Digests of Ribonucleic Acid

1965 ◽  
Vol 240 (10) ◽  
pp. 3984-3987
Author(s):  
George W. Rushizky ◽  
Isabelle H. Skavenski ◽  
Herbert A. Sober
Keyword(s):  
Ribonucleic Acid ◽  
Ribonuclease T1

SSMBS: a web server to locate sequentially separated motifs in biological sequences

2009 ◽  
Vol 43 (1) ◽  
pp. 203-205 ◽  
Author(s):  
Chetan Kumar ◽  
K. Sekar
Keyword(s):  
Amino Acids ◽  
Web Server ◽  
Nucleotide Sequences ◽  
Regular Expressions ◽  
Biological Sequences ◽  
Sequence Motifs ◽  
Specific Order ◽  
The Web

The identification of sequence (amino acids or nucleotides) motifs in a particular order in biological sequences has proved to be of interest. This paper describes a computing server,SSMBS, which can locate and display the occurrences of user-defined biologically important sequence motifs (a maximum of five) present in a specific order in protein and nucleotide sequences. While the server can efficiently locate motifs specified using regular expressions, it can also find occurrences of long and complex motifs. The computation is carried out by an algorithm developed using the concepts of quantifiers in regular expressions. The web server is available to users around the clock at http://dicsoft1.physics.iisc.ernet.in/ssmbs/.

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