scholarly journals Biosynthesis of N-(purin-6-ylcarbamoyl)-l-threonine riboside. Incorporation of l-threonine in vivo into modified nucleoside of transfer ribonucleic acid

1972 ◽  
Vol 127 (3) ◽  
pp. 515-519 ◽  
Author(s):  
Girish B. Chheda ◽  
Chung Il Hong ◽  
Conrad F. Piskorz ◽  
G. A. Harmon
Keyword(s):  
Escherichia Coli ◽  
Rat Liver ◽  
Ribonucleic Acid ◽  
Modified Nucleoside

l-[U-14C]Threonine is incorporated into N-(purin-6-ylcarbamoyl)-l-threonine riboside of rat liver and Escherichia coli tRNA. A pathway is suggested for the biosynthesis of this nucleoside.

scholarly journals Determination of the amount of small messenger ribonucleic acid-containing particles free in liver cytoplasm

1975 ◽  
Vol 152 (1) ◽  
pp. 51-56 ◽  
Author(s):  
B M Mullock ◽  
R H Hinton
Keyword(s):  
Rat Liver ◽  
Ribonucleic Acid ◽  
Messenger Ribonucleic Acid ◽  
Ribonucleoprotein Particles

To assess the contribution made by mRNA-containing particles to the heterogeneity previously observed among rat liver 40S ribonucleoprotein particles, the amount of poly(A)-containing RNA in subribosomal particles was determined. RNA was labelled with orotate in vivo for 24h and then for 50min. Poly(A)-containing RNA was trapped on filters impregnated with poly(U). Very little poly(A)-containing RNA was found in conventionally prepared ribonucleoprotein particles after fractionation in sucrose. However, after preparation of ribonucleoprotein particles by sedimentation through 1 M-sucrose in the presence of 0.15M-KCl or by precipitation with Mg2+ as described by Leitin & Lerman [(1969) Biokhimiya 34, 839-849], amounts of poly(A)-containing RNA were similar to amounts of mRNA found by other workers in total ribonucleoprotein particles. Even in such preparations, less than 5% of the total rapidly labelled RNA in native subribosomal-particle fractions was mRNA. It seems that mRNA-containing particles make up only a very small part of the population of subribosomal particles in liver.

3-(3-Amino-3-carboxypropyl)uridine. Novel modified nucleoside isolated from Escherichia coli phenylalanine transfer ribonucleic acid

Biochemistry ◽  
1974 ◽  
Vol 13 (12) ◽  
pp. 2620-2625 ◽  
Author(s):  
Ziro Ohashi ◽  
Mitsuaki Maeda ◽  
James A. McCloskey ◽  
S. Nishimura
Keyword(s):  
Escherichia Coli ◽  
Ribonucleic Acid ◽  
Modified Nucleoside

scholarly journals Transient growth inhibition of Escherichia coli K-12 by ion chelators: "in vivo" inhibition of ribonucleic acid synthesis.

1979 ◽  
Vol 138 (3) ◽  
pp. 923-932 ◽  
Author(s):  
J J Collins ◽  
C R Alder ◽  
J A Fernandez-Pol ◽  
D Court ◽  
G S Johnson
Keyword(s):  
Escherichia Coli ◽  
Growth Inhibition ◽  
Ribonucleic Acid ◽  
Acid Synthesis ◽  
Transient Growth ◽  
K 12
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