scholarly journals Ribonucleic acid–deoxyribonucleic acid hybridization in aqueous solutions and in solutions containing formamide

1971 ◽  
Vol 125 (2) ◽  
pp. 481-487 ◽  
Author(s):  
S. Gillespie ◽  
D. Gillespie
Keyword(s):  
Sodium Chloride ◽  
Aqueous Solutions ◽  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Sodium Citrate ◽  
Hybridization Reaction

Hybridization in 6×SSC (SSC, 0.15m-sodium chloride–0.015m-sodium citrate) at 66°C was compared with hybridization in formamide−6×SSC (1:1, v/v) at 35°C. As expected, the RNA hybridization potential was labile in the former system and stable in the latter. DNA retention by filters was poor in the formamide system, but could be improved. Several other properties of the hybridization reaction were explored and it was concluded that the formamide system is generally superior.

scholarly journals Interaction of polyanions with basic fuchsin and formaldehyde.

1977 ◽  
Vol 25 (1) ◽  
pp. 42-45 ◽  
Author(s):  
G L Carlson
Keyword(s):  
Aqueous Solutions ◽  
Ambient Temperature ◽  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Gel Filtration ◽  
Basic Fuchsin ◽  
Color Formation ◽  
Color Changes ◽  
Essential Aspect ◽  
Spectral Shifts

Basic fuchsin and formaldehyde react readily with a number of polyanions in aqueous solutions. Deoxyribonucleic acid, ribonucleic acid, several synthetic polynucleotides and polyvinylsulfate all convert buffered solutions of basic fuchsin and formaldehyde from a magenta to a purple color at ambient temperature. The formation of product is maximal in about 20 min and the resulting complexes are stable enough to be isolated by gel filtration. Microgram amounts of polyanion suffice to produce the color changes and the spectral shifts vary with the type of polyanion. These results are interpreted to indicate that polymerization of basic fuchsin and formaldehyde is an essential aspect of color formation.

scholarly journals The Enzymatic Phosphorylation of Ribonucleic Acid and Deoxyribonucleic Acid

1966 ◽  
Vol 241 (12) ◽  
pp. 2933-2943 ◽  
Author(s):  
Abraham Novogrodsky ◽  
Moshe Tal ◽  
Abraham Traub ◽  
Jerard Hurwitz
Keyword(s):  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Enzymatic Phosphorylation
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