scholarly journals The lactate dehydrogenase system of the rudd (Leuciscus erythrophthalmus)

1971 ◽  
Vol 125 (4) ◽  
pp. 113P-114P ◽  
Author(s):  
B M Buckley ◽  
T G Brady
Keyword(s):  
Lactate Dehydrogenase ◽  
Dehydrogenase System

scholarly journals Pressure relaxation of the equilibrium of the pig heart lactate dehydrogenase system

1978 ◽  
Vol 171 (1) ◽  
pp. 215-223 ◽  
Author(s):  
M J Hardman ◽  
J H Coates ◽  
H Gutfreund
Keyword(s):  
Relaxation Time ◽  
Lactate Dehydrogenase ◽  
Relaxation Times ◽  
Enzyme Concentration ◽  
Relaxation Behaviour ◽  
Pressure Perturbation ◽  
Protein Fluorescence ◽  
Pressure Relaxation ◽  
Dehydrogenase System ◽  
Total Enzyme

The relaxation behaviour of a system of reactants equilibrated in the presence of pig heart lactate dehydrogenase was studied after pressure perturbation. Two relaxations were observed when protein fluorescence was recorded, but only the slower relaxation was apparent in observations of A340. The faster relaxation therefore involves transfer between free and enzyme-bound NADH, whereas the slower relaxation represents the reduction of NAD+. Both relaxations were observed in Tris buffer, where there is little effect of pressure on pH, and in phosphate buffer, where pH changes are significant; however, the amplitudes depended on the buffer used. The slower reciprocal relaxation time increases with increasing total enzyme concentration and decreases slightly with increasing NAD+ concentration. Computer simulations, based on a proposed mechanism, were compared with the experimentally determined amplitudes and relaxation times as a test of the mechanism.

scholarly journals SPECIES- AND AGE-SPECIFIC CHARACTERISTICS OF THE LACTATE DEHYDROGENASE SYSTEM IN TISSUES OF RODENTS (MAMMALIA: RODENTIA)

2018 ◽  
pp. 3
Author(s):  
Екатерина Петровна Антонова ◽  
Светлана Николаевна Сергина ◽  
Виктор Александрович Илюха ◽  
Алина Евгеньевна Якимова ◽  
Ekaterina Antonova ◽  
...  
Keyword(s):  
Lactate Dehydrogenase ◽  
Dehydrogenase System

Source of Catalysis in the Lactate Dehydrogenase System. Ground-State Interactions in the Enzyme.cntdot.Substrate Complex

Biochemistry ◽  
1994 ◽  
Vol 33 (8) ◽  
pp. 2297-2305 ◽  
Author(s):  
Hua Deng ◽  
Jie Zheng ◽  
Anthony Clarke ◽  
John J. Holbrook ◽  
Robert Callender ◽  
...  
Keyword(s):  
Lactate Dehydrogenase ◽  
Ground State ◽  
System Ground State ◽  
Dehydrogenase System
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