scholarly journals Studies on the primary structure of chicken erythrocyte histone fraction V

1971 ◽  
Vol 124 (2) ◽  
pp. 319-325 ◽  
Author(s):  
P. J. Greenaway
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Primary Structure ◽  
Sequence Data ◽  
Acid Extraction ◽  
Chicken Erythrocyte ◽  
Histone Fraction

Histone fraction V was prepared by selective acid extraction from chicken erythrocyte nuclei. Amino acid sequence studies on the tryptic and thermolytic peptides are reported. Only a limited amount of overlapping sequence data was obtained.

scholarly journals Primary structure of the Streptomyces R61 extracellular DD-peptidase. 2. Amino acid sequence data

1987 ◽  
Vol 162 (3) ◽  
pp. 519-524 ◽  
Author(s):  
Bernard JORIS ◽  
Philippe JACQUES ◽  
Jean-Marie FRERE ◽  
Jean-Marie GHUYSEN ◽  
Jozef BEEUMEN
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Primary Structure ◽  
Sequence Data ◽  
Amino Acid Sequence Data

scholarly journals The stepwise removal of histones from chicken erythrocyte nucleoprotein

1968 ◽  
Vol 107 (2) ◽  
pp. 207-215 ◽  
Author(s):  
K. Murray ◽  
G. Vidali ◽  
J. M. Neelin
Keyword(s):  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Chicken Erythrocyte ◽  
Histone Fraction ◽  
Buffer Solutions ◽  
Avian Erythrocytes ◽  
Zone Electrophoresis ◽  
Exclusion Chromatography ◽  
Starch Gels

1. A fractionation of chicken erythrocyte histones was achieved simultaneously with their extraction from saline-washed nuclei by stepwise titrations to progressively lower pH values. 2. Different acids and dilute buffer solutions of comparable pH behaved similarly in stepwise extractions of histones. 3. The histone preparations so obtained were characterized by their amino acid composition and behaviour on zone electrophoresis in starch gels. 4. The fractionation by titration was quite sharp at appropriate pH ranges, and the histone fraction that is apparently unique to avian erythrocytes was obtained without contamination by other histone fractions. 5. Histones prepared by stepwise titration were fractionated further by cation-exchange and exclusion chromatography. The chromatographic behaviour and amino acid composition of the components permitted comparison with histones prepared by other methods. 6. Histone fraction IIb was resolved into its subfractions IIb1 and IIb2 by exclusion chromatography on Bio-Gel P-60. 7. Histone fractions III and IV, previously reported to be absent from chicken erythrocyte nuclei, were found in extracts made at pH1.

Sign in / Sign up

Export Citation Format

Share Document