scholarly journals Isolation and nature of intracellular peptides from a cephalosporin C-producing Cephalosporium sp

1971 ◽  
Vol 123 (3) ◽  
pp. 471-476 ◽  
Author(s):  
P. Bronwen Loder ◽  
E. P. Abraham
Keyword(s):  
Mass Spectrum ◽  
Amino Acid ◽  
Methyl Ester ◽  
Amino Acid Analysis ◽  
Paper Electrophoresis ◽  
Sulphonic Acid ◽  
Cephalosporin C ◽  
Acid Form ◽  
The Third ◽  
Thiol Form

1. Three peptides containing α-aminoadipic acid and cysteine have been obtained in small amounts from the mycelium of a Cephalosporium sp. 2. The peptides were precipitated as cuprous mercaptides together with glutathione and resolved from the latter and from each other by preparative paper electrophoresis and chromatography either in the sulphonic acid form or as S-sulphonyl derivatives. From the S-sulphonyl derivatives they were obtained in the thiol form. 3. One peptide (P3) was shown by amino acid analysis and the mass spectrum of the NS-ethoxycarbonyl derivative of its methyl ester to be δ-(l-α-aminoadipyl)-l-cysteinyl-d-valine. A second peptide (P2) contained α-aminoadipic acid, cysteine, valine and glycine, and the third peptide (P1) contained α-aminoadipic acid, cysteine, β-hydroxyvaline and glycine.

scholarly journals The inhibition of glutamate dehydrogenase by l-serine O-sulphate and related compounds and by photo-oxidation in the presence of Rose Bengal

1971 ◽  
Vol 123 (3) ◽  
pp. 421-426 ◽  
Author(s):  
N. Tudball ◽  
P. Thomas
Keyword(s):  
Amino Acid ◽  
Methyl Ester ◽  
Glutamate Dehydrogenase ◽  
Rose Bengal ◽  
Amino Acid Analysis ◽  
Inhibitory Effects ◽  
Irreversible Inhibitor ◽  
Photo Oxidation ◽  
Rapid Inactivation ◽  
Related Compounds

1. Glutamate dehydrogenase was inhibited by l-serine O-sulphate, β-chloro-l-alanine, O-phospho-l-serine and β-chloro-l-alanine methyl ester. With the exception of β-chloro-l-alanine methyl ester which was an irreversible inhibitor, it was possible to reverse the inhibitory effects by dialysis. 2. Both NAD+ and glutamate afford some protection against the inhibition due to the methyl ester. No change in the normal stimulatory effect exhibited by ADP was observed in the presence of β-chloro-l-alanine methyl ester but the effect due to GTP was modified. 3. Irradiation of glutamate dehydrogenase in the presence of Rose Bengal produced rapid inactivation. Amino acid analysis of the inactivated enzyme showed that eight histidine residues had been destroyed in the process.

An ESR study of the peroxidase reaction catalyzed by human methaemoglobin and methaemoglobin-haptoglobin complex

1991 ◽  
Vol 56 (4) ◽  
pp. 923-932
Author(s):  
Jana Stejskalová ◽  
Pavel Stopka ◽  
Zdeněk Pavlíček
Keyword(s):  
Hydrogen Peroxide ◽  
Ascorbic Acid ◽  
Amino Acid ◽  
Peroxidase Activity ◽  
Amino Acid Analysis ◽  
Superoxide Radical ◽  
Esr Spectra ◽  
The Other ◽  
Delocalized Electron

The ESR spectra of peroxidase systems of methaemoglobin-ascorbic acid-hydrogen peroxide and methaemoglobin-haptoglobin complex-ascorbic acid-hydrogen peroxide have been measured in the acetate buffer of pH 4.5. For the system with methaemoglobin an asymmetrical signal with g ~ 2 has been observed which is interpreted as the perpendicular region of anisotropic spectrum of superoxide radical. On the other hand, for the system with methaemoglobin-haptoglobin complex the observed signal with g ~ 2 is symmetrical and is interpreted as a signal of delocalized electron. After realization of three repeatedly induced peroxidase processes the ESR signal of the perpendicular part of anisotropic spectrum of superoxide radical is distinctly diminished, whereas the signal of delocalized electron remains practically unchanged. An amino acid analysis of methaemoglobin along with results of the ESR measurements make it possible to derive a hypothesis about the role of haptoglobin in increasing of the peroxidase activity of methaemoglobin.

scholarly journals Whole-Genome Sequences of Influenza A(H1N1)pdm09 Virus Isolates from Kerala, India

2017 ◽  
Vol 5 (28) ◽  
Author(s):  
Sara Jones ◽  
Raji Prasad ◽  
Anjana S. Nair ◽  
Sanjai Dharmaseelan ◽  
Remya Usha ◽  
...  
Keyword(s):  
Amino Acid ◽  
Amino Acid Analysis ◽  
Influenza A ◽  
Whole Genome Sequence ◽  
Whole Genome ◽  
H1n1 Pandemic ◽  
Genome Sequences ◽  
Influenza A H1n1 ◽  
Virus Isolates ◽  
New Mutations

ABSTRACT We report here the whole-genome sequence of six clinical isolates of influenza A(H1N1)pdm09, isolated from Kerala, India. Amino acid analysis of all gene segments from the A(H1N1)pdm09 isolates obtained in 2014 and 2015 identified several new mutations compared to the 2009 A(H1N1) pandemic strain.

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