scholarly journals The influence of the intracellular concentration on the uptake of l-[14C]valine by chopped tissue from cerebral cortex

1971 ◽  
Vol 123 (3) ◽  
pp. 341-345 ◽  
Author(s):  
C. T. Jones ◽  
P. Banks
Keyword(s):  
Cerebral Cortex ◽  
Electrical Stimulation ◽  
Guinea Pig ◽  
Creatine Phosphate ◽  
Intracellular Concentration ◽  
Sodium Potassium ◽  
Intracellular Concentrations

1. The intracellular concentrations of sodium, potassium, ATP and creatine phosphate in chopped tissue from guinea-pig cerebral cortex were altered by a 10min period of electrical stimulation. 2. The ability of the tissue to take up l-[14C]valine and incorporate it into protein was tested at various times after the cessation of electrical stimulation. 3. The restoration of the ability of the tissue to accumulate l-[14C]valine correlated closely with the restoration of the resting intracellular concentrations of sodium and ATP.

scholarly journals The effect of electrical stimulation and ouabain on the uptake and efflux of l-[U-14C]valine in chopped tissue from guinea-pig cerebral cortex

1970 ◽  
Vol 118 (5) ◽  
pp. 801-810 ◽  
Author(s):  
C. T. Jones ◽  
P. Banks
Keyword(s):  
Cerebral Cortex ◽  
Electrical Stimulation ◽  
Guinea Pig ◽  
Kinetic Analysis ◽  
Creatine Phosphate ◽  
Complete Loss ◽  
Extracellular Concentration ◽  
Prolonged Stimulation ◽  
Energy Dependent

1. Chopped tissue from guinea-pig cerebral cortex carried out an energy-dependent accumulation of l-[14C]valine. 2. The uptake was dependent on the extracellular concentration of Na+ and was markedly inhibited by ouabain (20μm). The extent of the inhibition of uptake by ouabain was also Na+-dependent. 3. The accumulation of labelled valine was not directly dependent on the ATP and creatine phosphate contents of the slices. 4. Electrical stimulation increased the rate of [14C]valine uptake at first but ultimately led to a net loss of the label so that the amount of label present in the tissue was lower than in the controls. 5. The rate of loss of label during prolonged stimulation was dependent on the extracellular concentration of Na+. 6. The efflux of labelled valine from slices preloaded at 164mm-Na+ was studied at 164, 80 and 40mm-Na+ with and without electrical stimulation or ouabain. 7. Lowering the Na+ concentration or adding ouabain increased the rate of efflux. 8. Electrical stimulation had little effect on the rate of efflux at first but ultimately led to a more complete loss of label from the tissue than occurred in the control. A kinetic analysis of the efflux curves was attempted.

scholarly journals The effect of electrical stimulation on the incorporation of l-[U-14C]valine into the protein of chopped tissue from guinea-pig cerebral cortex

1970 ◽  
Vol 118 (5) ◽  
pp. 791-800 ◽  
Author(s):  
C. T. Jones ◽  
P. Banks
Keyword(s):  
Cerebral Cortex ◽  
Electrical Stimulation ◽  
Guinea Pig ◽  
High Energy ◽  
Initial Increase ◽  
Nuclear Fraction ◽  
High Energy Phosphate ◽  
Experimental Conditions ◽  
Relative Contribution ◽  
Energy Dependent

1. Chopped tissue from guinea-pig cerebral cortex carried out an energy-dependent incorporation of [14C]valine into protein. 2. At all times studied the nuclear fraction of the homogenized tissue accounted for about 25% of the total labelled protein. 3. Electrical stimulation at first increased, but subsequently decreased, the rate of incorporation of [14C]valine into protein of the chopped tissue. 4. The initial increase in the incorporation of [14C]valine into protein occurred in the nuclear fraction. At later times electrical stimulation decreased the incorporation into all the subcellular fractions, but the relative contribution of the nuclear fraction to the total labelled protein increased. 5. These changes are discussed in relation to the changes in the rates of respiration, glycolysis, high-energy phosphate content and intracellular Na+ and K+ concentrations, which were measured under the same experimental conditions as those used to study protein synthesis.

XXV.—The Topography of the Cerebral Cortex of the Guinea-pig

1912 ◽  
Vol 31 ◽  
pp. 397-415 ◽  
Author(s):  
Williamina Abel
Keyword(s):  
Cerebral Cortex ◽  
Electrical Stimulation ◽  
Guinea Pig

This investigation was begun as a result of certain observations made by Dr T. Graham Brown, on reflexes in the guinea-pig (45). In various experiments he removed parts of the cerebral cortex and found that little if any disturbance, either motor or sensory, ensued. It was thereupon suggested that an examination should be made of the cortex histologically and by electrical stimulation in order to locate the position of the various areas.

Effects of Electrical Stimulation of the Superior Cervical Ganglion on Cochlear Blood Flow in Guinea Pig

1993 ◽  
Vol 113 (2) ◽  
pp. 146-151 ◽  
Author(s):  
Tian-Ying Ren ◽  
E. Laurikainen ◽  
W. S. Quirk ◽  
J. M. Miller ◽  
A. L. Nuttall
Keyword(s):  
Blood Flow ◽  
Electrical Stimulation ◽  
Guinea Pig ◽  
Superior Cervical Ganglion ◽  
Cochlear Blood Flow ◽  
Cervical Ganglion ◽  
Stimulation Of

scholarly journals Turnover of protein-bound serine phosphate in respiring slices of guinea-pig cerebral cortex. Effects of putative transmitters, tetrodotoxin and other agents

1973 ◽  
Vol 132 (3) ◽  
pp. 475-482 ◽  
Author(s):  
Martin Reddington ◽  
Richard Rodnight ◽  
Michael Williams
Keyword(s):  
Cerebral Cortex ◽  
Cyclic Amp ◽  
Guinea Pig ◽  
Electrical Pulses ◽  
Phosphorus Turnover ◽  
Serine Phosphate ◽  
Stimulation Of

1. The effect of various agents on the turnover of protein-bound phosphorus in respiring slices of cerebral cortex was studied. 2. Confirming previous work turnover was increased by the application of electrical pulses for 10s to the tissue. 3. Turnover was also increased by exposure of the slices for 10min to noradrenaline (0.5mm), 5-hydroxytryptamine (1μm) and histamine (0.1mm). 4. When slices were stimulated by electrical pulses in the presence of histamine the increase in turnover was the sum of the responses given by each agent above, suggesting that different phosphorylating systems were involved. 5. Tetrodotoxin (0.5μm) blocked the increased turnover due to electrical pulses, but not that due to histamine. Tetrodotoxin also prevented the increase in tissue cyclic AMP content caused by the application of electrical pulses. 6. Phosphoprotein turnover was not affected by adenosine, despite the increase in tissue cyclic AMP content given by this agent. 7. Adenosine blocked the phosphoprotein response to histamine, but did not affect the response to electrical pulses. 8. The results are discussed in relation to the hypothesis that the stimulation of protein phosphorus turnover by electrical pulses is secondary to the release of cyclic AMP in the tissue.

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