scholarly journals Androgen dynamics in vitro in the normal and hyperplastic human prostate gland

1971 ◽  
Vol 123 (1) ◽  
pp. 41-55 ◽  
Author(s):  
Eleonora P. Giorgi ◽  
Joan C. Stewart ◽  
J. K. Grant ◽  
R. Scott
Keyword(s):  
Steady State ◽  
Experimental Design ◽  
Prostate Gland ◽  
Human Prostate ◽  
Tissue Uptake ◽  
Uptake And Metabolism

The dynamics of uptake and metabolism in vitro of androgens by normal and hyperplastic human prostate glands was studied by means of a new experimental design proposed by Gurpide & Welch (1969). Prostate slices were perfused with a medium containing [3H]testosterone and [14C]androstenedione, or 5α-dihydro-[3H]testosterone and [14C]testosterone. The entry into the slices, the irreversible metabolism, the conversion between the compounds and the tissue retention or ‘uptake’ of the steroids were measured at the steady state. A similar portion of the three androgens entered the tissue and was irreversibly metabolized. Conversion of testosterone into 5α-dihydrotestosterone was much greater than the interconversion of testosterone and androstenedione. The prostate slices retained 5α-dihydrotestosterone at a concentration three times that in the medium, whereas testosterone and androstenedione were retained to a smaller extent. At a steroid concentration of 0.11μmol/l in the medium, the various parameters did not differ significantly in experiments performed with slices from normal and hyperplastic glands. When the steroid concentration in the medium was increased tenfold, however, a difference between normal and hyperplastic glands was evident. The normal glands increased the uptake and metabolism proportionally to the elevation of the steroid concentration in the medium. In the hyperplastic glands the entry and metabolism lagged behind the increase in steroid supply, whereas the tissue uptake became disproportionately high. The possible causes of this finding are discussed.

ANDROGEN DYNAMICS IN VITRO IN THE CANINE PROSTATE GLAND

1972 ◽  
Vol 55 (2) ◽  
pp. 421-439 ◽  
Author(s):  
ELEONORA P. GIORGI ◽  
J. K. GRANT ◽  
JOAN C. STEWART ◽  
JACQUELINE REID
Keyword(s):  
Steady State ◽  
Experimental Design ◽  
Prostate Gland ◽  
Prostatic Tissue ◽  
Similar Proportion ◽  
Uptake And Metabolism

SUMMARY The uptake and metabolism of androgens by canine prostate in vitro were studied by means of an experimental design which consisted of superfusion of prostate slices with a medium containing [4-14C]androstenedione and [6,7-3H]testosterone, or [4-14C]testosterone and 5α-dihydro[1,2-3H]testosterone. The entry or transfer of steroids into the slices, the irreversible metabolism, the conversion between the steroids and their uptake or tissue retention were measured at the steady state. A similar proportion of the three androgens entered the tissue and was irreversibly metabolized. Conversion of testosterone into 5α-dihydrotestosterone was much greater than the interconversion of testosterone and androstenedione. The prostate slices retained 5α-dihydrotestosterone in a concentration up to four times that in the medium, whereas testosterone and androstenedione were retained to a smaller extent. By increasing the steroid concentration in the perfusing medium from 0·03 to 1·30 μmol/l, it was shown that the systems regulating entry, uptake and metabolism of the androgens were not saturated within this range of concentration. No substantial differences were observed in the regulation of tissue—androgen interaction in vitro between five normal and four hyperplastic canine prostates. This was in contrast with previously reported findings that, in man, normal and hyperplastic prostatic tissue respond in a different way to an increase in steroid concentration in the medium.

scholarly journals Androgen dynamics in vitro in the human prostate gland. Effect of oestradiol-17β

1972 ◽  
Vol 126 (1) ◽  
pp. 107-121 ◽  
Author(s):  
E. P. Giorgi ◽  
J. C. Stewart ◽  
J. K. Grant ◽  
I. M. Shirley
Keyword(s):  
Steady State ◽  
Prostate Gland ◽  
Prostatic Tissue ◽  
Human Prostate ◽  
Oestrogen Treatment

Normal, hyperplastic and adenocarcinomatous human prostatic tissue was perfused in vitro with radioactively labelled androstenedione, testosterone and 5α-dihydrotestosterone with and without added oestradiol-17β. Various parameters of tissue–steroid relationship were measured at the steady state. When oestradiol (0.11 or 0.22μmol/l) was added to the perfusing medium, the entry of the steroids into the tissue and their metabolism was increased in the majority of the glands studied. The ‘uptake’ of all the steroids varied, in response to the addition of oestradiol, in both normal and adenocarcinomatous glands in a way differing from the response of hyperplastic glands. As a consequence, the tissue clearance of the steroids, particularly of androstenedione and testosterone, increased in normal and adenocarcinomatous glands in the presence of oestradiol, and decreased in the hyperplastic tissues. At a concentration 0.33μmol/l, oestradiol decreased the entry of the steroids in all the tissues studied, while the clearance of steroids tended to decrease. The significance of these findings in terms of the regulation of androgen dynamics in vivo in the normal and diseased human prostate, with particular regard to the response to oestrogen treatment, is discussed.

CHARACTERIZATION OF THE SPECIFIC ANDROGEN RECEPTORS IN THE HUMAN PROSTATE GLAND

1973 ◽  
Vol 57 (3) ◽  
pp. 371-384 ◽  
Author(s):  
W. I. P. MAINWARING ◽  
E. J. G. MILROY
Keyword(s):  
Binding Proteins ◽  
Androgen Receptors ◽  
Gradient Centrifugation ◽  
Prostate Gland ◽  
Sucrose Density Gradient ◽  
Human Prostate ◽  
Sucrose Density Gradient Centrifugation ◽  
Androgen Binding

SUMMARY A search has been conducted for specific androgen-binding proteins in soluble extracts of normal human prostate tissue and in surgical samples removed at retropubic prostatectomy for benign prostatic hyperplasia. Proteins with a particularly high affinity for a metabolite of testosterone, 5α-dihydrotestosterone, have been identified in studies on the binding of 3H-labelled steroids in vitro. The 5α-dihydrotestosterone-protein complexes have been partially characterized using sucrose density gradient centrifugation and gel-exclusion chromatography. The androgen receptors in the human prostate gland are remarkably similar to those previously described in the accessory sexual glands of experimental animals. These findings have been confirmed by the analysis of extracts of hyperplastic prostate glands labelled by the administration of [3H]testosterone in vivo. No attempt was made to correlate the degree of binding with the histological appearance of the specimens of hyperplastic prostate or to determine whether the receptors were principally present in the hyperplastic nodules. Such androgen-binding proteins were not present in serum, skeletal muscle or adipose tissue. The possible relevance of these findings to the onset of clinical disorders in the human prostate gland is briefly discussed.

scholarly journals Androgen dynamics in vitro in the human prostate gland. Effect of cyproterone and cyproterone acetate

1973 ◽  
Vol 132 (3) ◽  
pp. 465-474 ◽  
Author(s):  
Eleonora P. Giorgi ◽  
I. M. Shirley ◽  
J. K. Grant ◽  
Joan C. Stewart
Keyword(s):  
Cell Membrane ◽  
Cyproterone Acetate ◽  
Prostate Gland ◽  
Prostatic Tissue ◽  
Human Prostate ◽  
Specific Mechanism

Hyperplastic and adenocarcinomatous human prostatic tissue was superfused in vitro with radioactively labelled androst-4-ene-3,17-dione, testosterone and 5α-dihydrotestosterone (17β-hydroxy-5α-androstan-3-one), with and without addition of the anti-androgens cyproterone and cyproterone acetate. Cyproterone competitively inhibited the entry of the androgens into the majority of the tissues, whereas cyproterone acetate increased this entry. These findings indicated that transport of androstenedione, testosterone and 5α-dihydrotestosterone into prostatic tissue is performed by a specific mechanism, possibly involving a carrier situated in the cell membrane. The extent of metabolism of the three androgens was also modified: formation of 5α-dihydrotestosterone from testosterone, and of the latter from androstenedione, was decreased by cyproterone and increased by the acetate. Acetate was more effective than cyproterone in decreasing the `uptake' of the perfused androgens by the tissue; at the same time, it increased the androgen clearance from the tissue. As cyproterone acetate is the more potent of the two anti-androgens, the possibility that these findings in vitro are related to the different anti-androgenic potency exhibited by the two compounds in vivo is discussed. `Uptake' of the two anti-androgens and the response to their action on androgen dynamics were similar in adenocarcinomatous and hyperplastic glands.

Interaction between prolactin and zinc in the human prostate gland

1984 ◽  
Vol 102 (1) ◽  
pp. 73-76 ◽  
Author(s):  
A. Leake ◽  
G. D. Chisholm ◽  
F. K. Habib
Keyword(s):  
Specific Binding ◽  
Prostate Gland ◽  
Benign Prostatic Hypertrophy ◽  
Peptide Hormone ◽  
Prostatic Hypertrophy ◽  
Human Prostate ◽  
Binding Properties ◽  
Competitive Mechanism ◽  
Human Prolactin

ABSTRACT The interaction between prolactin and zinc was examined in vitro in the human prostate gland. The results indicated that prolactin did not modulate the acute uptake of zinc into benign prostatic hypertrophy tissue whereas zinc, in contrast, increased the uptake of prolactin into the prostate gland. Our study further showed that the augmented uptake of prolactin by zinc was partly due to an increase in the non-specific binding properties of the peptide hormone. We were also able to demonstrate that the specific binding of 125I-labelled human prolactin to the receptor was reduced in the presence of zinc by a competitive mechanism. J. Endocr. (1984) 102, 73–76

The Demonstration of Human Prostatic Acid Phosphatase (Pap) With Phosphorylcholine

1976 ◽  
Vol 34 ◽  
pp. 88-89
Author(s):  
José A. Serrano ◽  
Hannah L. Wasserkrug ◽  
Anna A. Serrano ◽  
Arnold M. Seligman
Keyword(s):  
Acid Phosphatase ◽  
Prostate Gland ◽  
Prostatic Acid Phosphatase ◽  
Human Prostate ◽  
Rat Tissues ◽  
Specific Substrate ◽  
Acid Phosphatases ◽  
Lysosomal Acid ◽  
Cacodylate Buffer

As previously reported (1, 2) phosphorylcholine (PC) is a specific substrate for prostatatic acid phosphatase (PAP) as opposed to other acid phosphatases, e.g., lysosomal acid phosphatase. The specificity of PC for PAP is due to the pentavalent nitrogen in PC, a feature that renders PC resistant to hydrolysis by all other acid phosphatases. Detailed comparative cytochemical results in rat tissues are in press. This report deals with ultracytochemical results applying the method to normal and pathological human prostate gland.Fresh human prostate was obtained from 7 patients having transurethral resections or radical prostatectomies. The tissue was fixed in 3% glutaraldehyde- 0.1 M cacodylate buffer (pH 7.4) for 15 min, sectioned at 50 μm on a Sorvall TC-2 tissue sectioner, refixed for a total of 2 hr, and rinsed overnight in 0.1 M cacodylate buffer (pH 7.4)-7.5% sucrose.

1442: CXCL12 Over-Expression and Secretion by Aging Fibroblasts Stimulates Human Prostate Epithelial Proliferation in Vitro

2006 ◽  
Vol 175 (4S) ◽  
pp. 466-466
Author(s):  
Jill A. Macoska ◽  
Lesa Begley ◽  
Christine Monteleon ◽  
James W. MacDonald ◽  
Rajal B. Shah
Keyword(s):  
Human Prostate ◽  
Epithelial Proliferation ◽  
Over Expression ◽  
Proliferation In Vitro
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