scholarly journals The effect of oestradiol on the acid-soluble nucleotides of rat uterus

1970 ◽  
Vol 119 (2) ◽  
pp. 187-191 ◽  
Author(s):  
Janet M. Oliver ◽  
A. E. Kellie
Keyword(s):  
Fine Powder ◽  
Hormone Treatment ◽  
Two Dimensional ◽  
Purine Nucleotides ◽  
Rat Uterus ◽  
Pyrimidine Nucleotides ◽  
Immature Rat ◽  
Short Columns ◽  
Layer Chromatography

1. Techniques have been developed to measure the concentrations of the ribonucleotides of the immature rat uterus in vivo. Tissue was frozen rapidly in liquid nitrogen, ground to a fine powder, dispersed in frozen perchloric acid and thawed slowly. Nucleotides were separated from other acid-soluble constituents on short columns of polyethyleneimine-cellulose and the mixture was resolved into individual nucleotides by two-dimensional thin-layer chromatography on polyethyl-eneimine-cellulose plates. 2. The nucleotides of immature rat uterus consisted of approximately 75% of ATP–ADP, 10–12% each of GTP–GDP and UTP–UDP and less than 2% of CTP. 3. Injection of oestradiol (5μg) promoted a linear decrease in the amounts of purine nucleotides to approximately 60% of control values in 4–5h, followed by a return to greater than control values in 8–10h. Concentrations of the pyrimidine nucleotides remained constant for 4–6h and then increased to 200% of control at 12h after hormone treatment.

scholarly journals Changes in the patterns of synthesis of ribonucleic acid species in immature rat uterus in response to oestradiol-17β

1969 ◽  
Vol 112 (5) ◽  
pp. 563-569 ◽  
Author(s):  
R J Billing ◽  
B Barbiroli ◽  
R. M. S. Smellie
Keyword(s):  
Ribosomal Rna ◽  
Hormone Treatment ◽  
Transfer Rna ◽  
Time Intervals ◽  
Rat Uterus ◽  
Immature Rat ◽  
Oestradiol Treatment ◽  
Wet Weight ◽  
Major Increase ◽  
Rna And Dna

1. After treatment of immature rats with diethylstilboestrol, the wet weight and RNA content of uterine tissue increased rapidly, reaching a peak at 40hr. After an initial lag of a few hours, the acid-soluble ribose and protein contents also rose to maxima at 40hr. No increase in DNA content occurred until at least 24hr. after treatment. 2. The RNA from immature rat uterus isolated at various times up to 6hr. after administration of oestradiol-17β was labelled by injecting [3H]uridine and [3H]guanosine intraperitoneally 30min. before the animals were killed. It was fractionated on columns of kieselguhr coated with methylated serum albumin and the radioactivity in fractions corresponding to transfer RNA, 7s RNA, ribosomal RNA, Q1-RNA, Q2-RNA and DNA-like RNA was determined. 3. The radioactivity of the whole RNA increased steadily for 6hr. after hormone treatment. The earliest changes occurred in the Q1-RNA (ribosomal RNA precursor), whereas at longer time-intervals the radioactivity of the ribosomal RNA, 7s RNA and transfer RNA increased by four- to five-fold. The radioactivity of the DNA-like RNA increased by about 50%, but only at the longer time-intervals. 4. It is concluded that one of the earliest changes in response to oestradiol treatment is a major increase in synthesis of ribosomal RNA followed later by a similar increase in synthesis of transfer RNA and by a much smaller increase in synthesis of DNA-like RNA. The change in synthesis of ribosomal RNA in immature rat uterus may represent one of the most important responses to oestradiol treatment.

Effects of Tokishakuyakusan, Keishibukuryogan and Unkeito on DNA Polymerase α Activity in PMS-treated Immature Rat Uterus Incubated in Vitro

1992 ◽  
Vol 20 (03n04) ◽  
pp. 265-268 ◽  
Author(s):  
Satoshi Usuki
Keyword(s):  
Enzyme Activity ◽  
Dna Polymerase ◽  
Control Groups ◽  
Rat Uterus ◽  
Immature Rat ◽  
Dna Polymerase Α ◽  
Significant Difference ◽  
Α Activity

We have recently found that Tokishakuyakusan (TS), Keishibukuryogan (KB) or Unkeito (UT) inhibits in vivo DNA polymerase α activity in the rat uterus stimulated by PMS. In this study, uteri resected 24 h after injection of PMS on day 27 of age were incubated in vitro with 20 μg/ml of extract of TS, KB or UT for 4 h. The DNA polymerase α activity in uteri tended to decrease after the addition of TS, KB or UT with significant difference ( P < 0.05) compared with TS-, KB- and UT-untreated control groups. These results suggest that TS, KB or UT, especially KB, tends to inhibit directly the enzyme activity in rat uterus.

scholarly journals In vivo Effects of Estetrol on the Immature Rat Uterus

1979 ◽  
Vol 20 (2) ◽  
pp. 242-246 ◽  
Author(s):  
C. F. Holinka ◽  
E. Gurpide
Keyword(s):  
Rat Uterus ◽  
Immature Rat ◽  
In Vivo Effects

Comparative pharmacology of oestrogens and catechol oestrogens: actions on the immature rat uterus in vivo and in vitro

1982 ◽  
Vol 94 (1) ◽  
pp. 91-98 ◽  
Author(s):  
S. Franks ◽  
N. J. MacLusky ◽  
F. Naftolin
Keyword(s):  
Histological Examination ◽  
Target Organ ◽  
Peripheral Target ◽  
Uterine Weight ◽  
Rat Uterus ◽  
Immature Rat ◽  
In Vivo Effects

The effects of primary and catechol oestrogens on the uterus of the immature rat were compared. Because differences between the in-vivo and in-vitro oestrogenic actions of catechol oestrogens on the secretion of LH had been observed, their effects on a peripheral target organ, the uterus, were examined under similar conditions. In-vivo effects were assessed by measurement of uterine weight, induction of uterine cytoplasmic progestogen receptors, and by histological examination. In-vitro actions were determined by measurement of oestrogen-specific induced protein. It was found that the uterotrophic effects in vivo of 4-hydroxyoestradiol were indistinguishable from those of oestradiol whereas 2-hydroxyoestradiol was only weakly oestrogenic and 2-hydroxyoestrone had no effect. However, in vitro, 2-hydroxyoestradiol was as effective as 4-hydroxyoestradiol or oestradiol in stimulating synthesis of uterine induced protein, and 2-hydroxyoestrone, although less potent than oestradiol, had a significant effect. These results were consistent with the observed effects on the secretion of LH. The differences between in-vivo and in-vitro uterotrophic properties of catechol oestrogens can be explained on the basis of known pharmacokinetic factors.

Theophylline-estrogen interaction in the rat uterus: role of the ovary

1982 ◽  
Vol 242 (2) ◽  
pp. E121-E126 ◽  
Author(s):  
J. Steinsapir ◽  
A. M. Rojas ◽  
A. Tchernitchin
Keyword(s):  
Protein Content ◽  
Rat Uterus ◽  
Immature Rat ◽  
Estrogen Binding

Uterine edema induced by 0.1 microgram/100 g body wt of estradiol further increases in the presence of theophylline. Theophylline alone or in the presence of 0.001, 0.01, 0.1, and 1 microgram/100 g body wt of estradiol increases uterine RNA and protein content 6 h after its administration, as compared with the same doses of estradiol alone. Both phenomena disappear in the ovariectomized immature rat, suggesting that theophylline potentiates the action of gonadotropins, increasing the synthesis of endogenous ovarian estrogens by the immature ovary. Theophylline decreases the number of eosinophils in the blood and concurrently decreases estrogen-induced uterine eosinophilia at doses of 0.01, 0.1, 1, 10, and 30 micrograms/100 g body wt of estradiol. Estrogen binding by uterine eosinophils in vitro increases in the presence of theophylline. This effect of theophylline could also explain the increase in vivo in estrogen-induced uterine edema.

Specific Labeling of Protein Domains with Antibody Fragments

1981 ◽  
Vol 39 ◽  
pp. 416-417
Author(s):  
U. Aebi ◽  
L.E. Buhle ◽  
W.E. Fowler
Keyword(s):  
Image Processing ◽  
Specific Protein ◽  
Antibody Fragments ◽  
Supramolecular Organization ◽  
Two Dimensional ◽  
Ordered Structures ◽  
Virus Capsids ◽  
Processing Techniques

Many important supramolecular structures such as filaments, microtubules, virus capsids and certain membrane proteins and bacterial cell walls exist as ordered polymers or two-dimensional crystalline arrays in vivo. In several instances it has been possible to induce soluble proteins to form ordered polymers or two-dimensional crystalline arrays in vitro. In both cases a combination of electron microscopy of negatively stained specimens with analog or digital image processing techniques has proven extremely useful for elucidating the molecular and supramolecular organization of the constituent proteins. However from the reconstructed stain exclusion patterns it is often difficult to identify distinct stain excluding regions with specific protein subunits. To this end it has been demonstrated that in some cases this ambiguity can be resolved by a combination of stoichiometric labeling of the ordered structures with subunit-specific antibody fragments (e.g. Fab) and image processing of the electron micrographs recorded from labeled and unlabeled structures.

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