scholarly journals Quantitative and metabolic changes of hepatic collagens in rats after carbon tetrachloride poisoning

1970 ◽  
Vol 118 (2) ◽  
pp. 229-232 ◽  
Author(s):  
Chisato Hirayama ◽  
Ikuo Morotomi ◽  
Kaichiro Hiroshige
Keyword(s):  
Carbon Tetrachloride ◽  
Rat Liver ◽  
Collagenolytic Activity ◽  
Normal Range ◽  
Carbon Tetrachloride Poisoning ◽  
Soluble Collagen ◽  
Insoluble Collagen ◽  
Acid Soluble Collagen ◽  
Soluble Acid

1. The collagen hydroxyproline in rat liver was composed of 3.5% neutral-soluble collagen, 4.9% acid-soluble collagen and 91.6% insoluble collagen. In labelling studies with [14C]proline in vitro, the specific radioactivities of neutral-soluble, acid-soluble and insoluble collagens in rat liver were found to be 233000, 69000 and 830d.p.m./μmol of hydroxyproline respectively after 1h. 2. During subacute carbon tetrachloride poisoning the hepatic content of insoluble collagen markedly increased, whereas those of soluble collagens did not change. During recovery from subacute poisoning hepatic contents of soluble collagens were markedly decreased. 3. After 8 weeks of carbon tetrachloride poisoning the specific radioactivities of hepatic soluble collagens increased, while that of insoluble collagen decreased. During recovery from subacute poisoning, the specific radioactivities of soluble collagens decreased to the normal range and that of insoluble collagen further decreased. 4. Hepatic collagenolytic activity solubilizing insoluble collagen, which differs from mammalian collagenase, decreased under the conditions of the subacute poisoning and also during recovery from subacute poisoning.

scholarly journals Hepatic collagenolytic activity in rats after carbon tetrachloride poisoning

1969 ◽  
Vol 115 (4) ◽  
pp. 843-847 ◽  
Author(s):  
Chisato Hirayama ◽  
Kaichiro Hiroshige ◽  
Tomiichi Masuya
Keyword(s):  
Single Dose ◽  
Carbon Tetrachloride ◽  
Free Amino ◽  
Supernatant Fraction ◽  
Acid Proteinase ◽  
Collagenolytic Activity ◽  
Hydroxyproline Content ◽  
Experimental Conditions ◽  
Carbon Tetrachloride Poisoning ◽  
Acid Soluble Collagen

1. Collagenolytic activity towards acid-soluble collagen labelled with [14C]-proline was assayed in rat liver with and without carbon tetrachloride poisoning. The products of enzymic digestion were found to be free amino acids and peptides. 2. The hepatic collagenolytic activity increased under conditions of single-dose and subacute carbon tetrachloride poisoning, and correlated with hydroxyproline content. The highest activity was found during recovery from subacute poisoning. 3. Under the same experimental conditions, hepatic acid-proteinase activity changed independently of the collagenolytic activity and also of hepatic hydroxyproline content. 4. The increased collagenolytic activity during carbon tetrachloride poisoning was found mainly in the supernatant fraction. 5. The ratio of the collagenolytic activity to hepatic hydroxyproline content increased during recovery from single-dose and subacute poisoning, and decreased during subacute poisoning.

Comparison of metabolic effects of carbon tetrachloride and 1,2-dichloroethane added in vitro to slices of rat liver

1989 ◽  
Vol 3 (1) ◽  
pp. 59-68 ◽  
Author(s):  
L. Thomas ◽  
B. DeFeo ◽  
M.F. Mariani ◽  
G.D.V. van Rossum
Keyword(s):  
Carbon Tetrachloride ◽  
Rat Liver ◽  
Metabolic Effects

scholarly journals Chemical changes associated with aging of collagen in vivo and in vitro

1969 ◽  
Vol 112 (4) ◽  
pp. 397-405 ◽  
Author(s):  
Kalindi Deshmukh ◽  
Marcel E. Nimni
Keyword(s):  
Absorption Spectrum ◽  
Neutral Salt ◽  
Unsaturated Aldehyde ◽  
Salt Solutions ◽  
Rat Skin ◽  
Soluble Collagen ◽  
Insoluble Collagen ◽  
Cross Links

Collagen extracted from rat skin by neutral-salt solutions contains less aldehydes than the more insoluble collagen fractions. The concentration of aldehydes in collagen is directly related to its capacity to form stable cross-linked gels, which do not redissolve on cooling and become more insoluble in a variety of reagents. Whereas the absorption spectrum of neutral-salt-soluble collagen treated with N-methylbenzothiazolone hydrazone resembles that of acetaldehyde, the more insoluble collagen fractions show increasing amounts of a component that behaves like an αβ-unsaturated aldehyde. The ratio between α- and β-sub-units present in a particular fraction of soluble collagen seems to be constant and independent of the age of the animal. Neutral-salt-soluble collagen, which has a low concentration of β-components, will generate intramolecular bonds if gelled at 37°. These intramolecular bonds seem to precede the formation of stable intermolecular cross-links, since these gels can redissolve when cooled to yield a soluble collagen with a higher content of β-components of intramolecular origin.

Effect of a low protein diet on restoration of the rat liver parenchyma after carbon tetrachloride poisoning

1988 ◽  
Vol 105 (3) ◽  
pp. 397-400
Author(s):  
G. N. Shorina ◽  
N. P. Bgatova ◽  
I. Simek ◽  
Z. Červinkova ◽  
M. Holeček ◽  
...  
Keyword(s):  
Carbon Tetrachloride ◽  
Rat Liver ◽  
Liver Parenchyma ◽  
Protein Diet ◽  
Low Protein Diet ◽  
Carbon Tetrachloride Poisoning ◽  
Low Protein
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