scholarly journals Synthesis of glutamate in Aerobacter aerogenes by a hitherto unknown route

1970 ◽  
Vol 117 (2) ◽  
pp. 405-407 ◽  
Author(s):  
D W Tempest ◽  
J L Meers ◽  
C M Brown
Keyword(s):  
Aerobacter Aerogenes ◽  
Unknown Route

scholarly journals PATHWAYS OF GLYCEROL DISSIMILATION IN TWO STRAINS OF AEROBACTER AEROGENES: ENZYMATIC AND TRACER STUDIES

1957 ◽  
Vol 226 (2) ◽  
pp. 891-899 ◽  
Author(s):  
David Rush ◽  
Doris Karibian ◽  
Manfred L. Karnovsky ◽  
Boris Magasanik
Keyword(s):  
Tracer Studies ◽  
Aerobacter Aerogenes

scholarly journals Cellobiose Metabolism in Aerobacter aerogenes

1972 ◽  
Vol 247 (11) ◽  
pp. 3420-3423
Author(s):  
Richard E. Palmer ◽  
Richard L. Anderson
Keyword(s):  
Aerobacter Aerogenes ◽  
Cellobiose Metabolism

Effect of metabolites on ε-N-hydroxylysine formation in cell-free extracts of Aerobacter aerogenes 62-1

1977 ◽  
Vol 55 (6) ◽  
pp. 625-629 ◽  
Author(s):  
G. J. Murray ◽  
G. E. D. Clark ◽  
M. A. Parniak ◽  
T. Viswanatha
Keyword(s):  
Enzymatic Activity ◽  
Hydroxy Derivative ◽  
Differential Centrifugation ◽  
Hydroxylase Activity ◽  
Aerobacter Aerogenes ◽  
Lactate And Pyruvate ◽  
First Time

The conversion of L-lysine to its corresponding ε-N-hydroxy derivative has been achieved for the first time by cell-free extracts of Aerobacter aerogenes 62-1. Partial fractionation by differential centrifugation (at 12 000 × g) revealed that both supernatant and pellet are essential for maximum enzymatic activity. The ω-N-hydroxylase (EC 1.14.99) was found to function optimally at pH 7–7.5 and exhibited an apparent Km of about 75 μM for L-lysine. L(+)-Lactate or DL-lactate and pyruvate greatly stimulate the ω-N-hydroxylase activity. The system is strongly inhibited by arsenite and sulfite.

Nitrate reduction in Aerobacter aerogenes

1967 ◽  
Vol 58 (3) ◽  
pp. 228-247 ◽  
Author(s):  
A. H. Stouthamer ◽  
Corry Bettenhausen ◽  
J. Hartingsveldt ◽  
J. van't Riet ◽  
R. J. Planta
Keyword(s):  
Nitrate Reduction ◽  
Aerobacter Aerogenes

Nitrogen fixation by cell-free extracts of Klebsiella pneumoniae

1968 ◽  
Vol 14 (1) ◽  
pp. 33-38 ◽  
Author(s):  
M. C. Mahl ◽  
P. W. Wilson
Keyword(s):  
Nitrogen Fixation ◽  
Klebsiella Pneumoniae ◽  
Azotobacter Vinelandii ◽  
Ammonium Ion ◽  
Michaelis Constant ◽  
Nitrogen Gas ◽  
Aerobacter Aerogenes ◽  
Free System ◽  
Evolving System ◽  
A Cell

A cell-free system which permits nitrogen fixation by extracts of Klebsiella pneumoniae M5al (formerly Aerobacter aerogenes) has been developed. It is, essentially, that system described by Bulen and associates for Azotobacter vinelandii, utilizing ATP as a source of energy and dithionite as a source of electrons. The Michaelis constant for fixation has been estimated to be 0.12 atm. The extracts possessed an ATP-dependent hydrogen evolving system. Hydrogen evolution from these extracts was less under nitrogen than under helium in the presence of ATP. Nitrogen gas appears to be the inducer of nitrogen fixation. In the absence of N2, no induction of nitrogenase occurs. Nitrogenase is absent in cells grown on NH4+-N. There is a lag of about 13 h after the introduction of N2 gas into a culture which has depleted its supply of NH4+-N before nitrogenase can be detected. For reasons discussed in the text, this conclusion must be regarded as tentative at this time. Ammonium ion appears to prevent the synthesis of new molecules of nitrogenase without affecting the activity of those already formed.

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